Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	481,421	C→A	A995S (GCT→TCT)	acrB ←	multidrug efflux pump RND permease AcrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	481,421	0	C	A	100.0%	138.4 / NA	37	A995S (GCT→TCT)	acrB	multidrug efflux pump RND permease AcrB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (24/13);  total (24/13)

ACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGTCATCAGGATCGGA  >  NC_000913/481357‑481485                                                                 |                                                                 aCCGTTGCGGTCACGATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGAAcc                                                               >  1:55295/1‑68 (MQ=255) aCCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGAAcc                                                               >  1:3153959/1‑68 (MQ=255) aCCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGAAcc                                                               <  1:247420/68‑1 (MQ=255) aCCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGAAcc                                                               <  1:1437028/68‑1 (MQ=255)   cGTTGCGGTCACCATCCCGCCCCTTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGAAACAg                                                             >  1:2345752/1‑68 (MQ=38)       gCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGAACCAGTACt                                                         <  1:804806/68‑1 (MQ=255)       gCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGAACCAGTACt                                                         <  1:446213/68‑1 (MQ=255)       gCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGAACCAGTACt                                                         <  1:1484172/68‑1 (MQ=255)         ggTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGAACCAGTACTGa                                                       >  1:2543807/1‑68 (MQ=255)         ggTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGAACCAGTACTGa                                                       >  1:849506/1‑68 (MQ=255)         ggTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGAACCAGCACTGa                                                       >  1:3526410/1‑68 (MQ=255)           tCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGAACCAGTACTGATa                                                     <  1:1554232/68‑1 (MQ=255)                    gacccATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGAACCAGTACTGATAACCAGCGGc                                            <  1:3273628/66‑1 (MQ=255)                    cGCCCATTACACCGGTACCTCCTGCGTTCTGCGCGCCGGAACCAGAACCAGTACTGATAACCagcagc                                            <  1:430183/68‑1 (MQ=21)                    cGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGAACCAGTACTGATAACCAGCGGc                                            <  1:3288490/68‑1 (MQ=255)                    cGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGAACCAGTACTGATAACCAGCGGc                                            <  1:2486872/68‑1 (MQ=255)                       ccATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGAACCAGTACTGATAACCAGCGGCATa                                         >  1:943810/1‑68 (MQ=255)                          ttACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGAACCAGTACTGATAACCAGCGGCATAACg                                      <  1:877240/68‑1 (MQ=255)                              acCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGAACCAGTACTGATAACCAGCGGCATAACGCCGa                                  >  1:1078287/1‑68 (MQ=255)                               ccGGTACCTACTGCGTTCTGCGCGACGGAACCAGAACCAGTCATGATAACCAGCGGAATAACGCCgcg                                 >  1:2871100/1‑66 (MQ=21)                                cGGTACCTACTGCGTTCTGCGCGCCGNAACCAGAACCAGTACTGATAACCAGCGGCATAACGCCGAgg                                <  1:2868762/68‑1 (MQ=255)                                cGGTACCTACTGCGTTCTGCGCGCCGGAACCAGAACCAGTACTGATAACCAGCGGCATAACGCCGAgg                                <  1:828432/68‑1 (MQ=255)                                 ggTACCTACTGCGTTCTGCGCGCCGGAACCAGAACCAGTACTGATAACCAGCGGCATAACGCCGAGGa                               <  1:538204/68‑1 (MQ=255)                                 ggTACCTACTGCGTTCTGCGCGCCGGAACCAGAACCAGTACTGATAACCAGCGGCATAACGCCGAGGa                               >  1:1251048/1‑68 (MQ=255)                                    aCCTACTGCGTTCTGCGCGCCGGAACCAGAACCAGTACTGATAACCAGCGGCATAACGCCGAGGATaa                            >  1:3232963/1‑68 (MQ=255)                                    aCCTACTGCGTTCTGCGCGCCGGAACCAGAACCAGTACTGATAACCAGCGGCATAACGCCGAGGATaa                            >  1:2025298/1‑68 (MQ=255)                                    aCCTACTGCGTTCTGCGCGCCGGAACCAGAACCAGTACTGATAACCAGCGGCATAAAGCCGAGGATaa                            >  1:2659173/1‑68 (MQ=25)                                      cTACTGCGTTCTGCGCGCCGGAACCAGAACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAAc                          >  1:1457068/1‑68 (MQ=255)                                      cTACTGCGTTCTGCGCGCCGGAACCAGAACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAAc                          >  1:3332115/1‑68 (MQ=255)                                      cTACTGCGTTCTGCGCGCCGGAACCAGAACCAGTACTGACAACCAGCGGCATAACGCCGAGGATAAAc                          >  1:1639724/1‑68 (MQ=255)                                        aCTGCGTTCTGCGCGCCGGAACCAGAACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGc                        >  1:2077875/1‑68 (MQ=255)                                        aCTGCGTTCTGCGCGCCGGAACCAGAACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGc                        >  1:1300639/1‑68 (MQ=255)                                         cTGCGTTCTGCGCGCCGGAACCAGAACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGcc                       >  1:2779495/1‑68 (MQ=255)                                           gCGTTCTGCGCGCCGGAACCAGAACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAg                     >  1:2179355/1‑68 (MQ=255)                                            cGTTCTGCGCGCCGGAACCAGAACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGc                    >  1:2744685/1‑68 (MQ=255)                                             gTTCTGCGCGCCGGAACCAGAACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCg                   >  1:3550231/1‑68 (MQ=255)                                             gTTCTGCGCGCCGGAACCAGAACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCg                   >  1:1730563/1‑68 (MQ=255)                                             gTTCTGCGCGCCGGAACCAGAACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCg                   >  1:1615359/1‑68 (MQ=255)                                               tCTGCGCGCCGGAACCAGAACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAg                 >  1:703690/1‑68 (MQ=255)                                               tCTGCGCGCCGGAACCAGAACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAg                 >  1:139254/1‑68 (MQ=255)                                                         ggAACCAGAACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGTCATCAGGa       <  1:1309669/68‑1 (MQ=255)                                                              cAGAACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGTCATCAGGATCGGa  >  1:3560240/1‑68 (MQ=255)                                                              cAGAACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGTCATCAGGATCGGa  >  1:2254636/1‑68 (MQ=255)                                                                 |                                                                 ACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGTCATCAGGATCGGA  >  NC_000913/481357‑481485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑