| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 395,294 | 0 | C | G | 31.8% | 31.1 / 20.9 | 22 | G332R (GGC→CGC) | ampH | peptidoglycan DD‑carboxypeptidase/peptidoglycan DD‑endopeptidase |
| Reads supporting (aligned to +/- strand): ref base C (10/5); new base G (7/0); total (17/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.35e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.64e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GACCACCACAAACGCGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCTGTCTTC‑TGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATACCC > NC_000913/395232‑395353 | gACCACCACAAACGCGCCAATATTCTTCTGCGGGATCATCGCCATATAGTTAATGAAACCGccac > 1:473402/1‑65 (MQ=255) ccaccaCAAACGCGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGATACGGcgacca > 1:1374680/1‑65 (MQ=255) ccaccaCAAACGCGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGccacca > 1:2641165/1‑65 (MQ=255) ccaccaCAAACGCGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGccacca > 1:3769657/1‑65 (MQ=255) ccaccaCAAACGCGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGccacca < 1:1728063/65‑1 (MQ=255) accacAAACGCGCCAATATTCTCCGGGGGGATCATCGCCATATAGGTAATAAACCCGCCcccacc < 1:992089/65‑1 (MQ=255) cgcgCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGATACGGCGACCACCGCGTGcct > 1:3576890/1‑65 (MQ=255) cgcgCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCTGtct < 1:1216648/65‑1 (MQ=255) cgcgCCAATATTCTTCTGCGGGATCATCGCCATATAGGCAATGAAACCGCGACCACCGCCCGtct > 1:2329440/1‑65 (MQ=255) cgcgCAAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCctctct > 1:1489192/1‑65 (MQ=255) taaTATTCTTCTGCGGGACCATCGCCATATAGGTAATGAAACCGCCACCACCGCCTGTCTTC‑TGa < 1:3106237/64‑1 (MQ=255) cAATATCCTTCTGCGGGATCATCGCCATATAGGTAATGAACCCGCCACCACCGCCTGTCTTC‑TGa < 1:3574806/65‑1 (MQ=255) aaaTATTCTTCTGCGGGATCATCGCCATATAGGTAATGAACCCGCGACCACCGCCTGTCTTC‑TGa < 1:764270/64‑1 (MQ=255) ttcttcTGGGGGATCCTCGCAATATAGGTAATGAAACCGCCACCACCGCCTGTCTTC‑TGaataat < 1:3066180/65‑1 (MQ=255) ttcttcTGGGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCTGTCTTC‑TGaataat < 1:518463/65‑1 (MQ=255) ttcttcTGCGGGATCATCGCCATATAGGTAATGAACCCGCCACCACCGCCTGTCTTC‑TGaataat < 1:3567808/65‑1 (MQ=255) ttcttcGGCGGGATCATCGCCATATAGGTAATAAACCCGCCACCACCGCCTGTCTTC‑TGaataat < 1:3148062/65‑1 (MQ=255) tcttctGCGGGATCATCGCCATATAGGTAATGATACGGCGACCACCGCCTGTCTTC‑TGAATAATc > 1:3816351/1‑65 (MQ=255) tcttctGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCTGTCTTC‑TGAATAATc > 1:835522/1‑65 (MQ=255) tcttctGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCTGTCTTC‑TGAATAATc < 1:3288828/65‑1 (MQ=255) tcttctGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCTGTCTTC‑TGAACAATc > 1:994554/1‑65 (MQ=255) ggCGGGATCATCGCCATATAGGTAATGAAACCGCCACCGCCGCCTGTCTTC‑TGAATAATCCCCgg < 1:501009/64‑1 (MQ=255) agggggACCATCGCCATATAGGAAATGAAACCGCCACCCCCGCCTGTCTTC‑TGAATAATCCCCgg < 1:838545/62‑1 (MQ=255) gaccatcGCCAAATAGGAAATAAAACCGCCTCCACCGCCTGTCTTC‑TGAATAATCCCCGgacgac < 1:1246099/62‑1 (MQ=255) gATCATCGCCATAGAGGTAATGAAACCGCCCCCACCGCCTGTCTTC‑TGAATAATCCCCGgacgac < 1:3417889/65‑1 (MQ=255) caccatcGCCTTATAGATAATCAGTCCGCCCCCACCGCCTGTCTTC‑TGAATAATCCCCGgacgac < 1:2955509/62‑1 (MQ=255) catcGCCATATAGGTAATGAACCCGCCCCCACCGCCTGTCTTC‑TGAATAATCCCCGGACGACCct < 1:2633786/65‑1 (MQ=255) tataGGTAATGATACGGCGACCACCG‑AGATCTACACGACTAATCCCCGCACGCCCCTCTTTCggg > 1:3356144/1‑65 (MQ=255) ataGGTAATGATACGGCGACCACCGCCTGTCTTC‑TGAATAATCCCCGGACGACCCTCTTTCgggg > 1:3849350/1‑65 (MQ=255) ataGGTAATGATACGGCGACCACCGACTGCTTTC‑CGAATAATCCCCGGACGACCCTCTTTCgggg > 1:2445252/1‑65 (MQ=255) ataGGTAATGAAACCGCCACCACCGCCTGTCTTC‑TGAATAATCCCCGGACGACCCTCTTTCgggg > 1:1549281/1‑65 (MQ=255) taGGTAATAAACCCGCCACCACCGCCTGTCTTC‑TGAATAATCCCCGGACGACCCTCTTTCGGGGc < 1:3882076/65‑1 (MQ=255) ggTAATGATACGGCGACCACCGCGTGACTTC‑TGACTACTCCCCGGACGCGCCTCTTTCggggtcc > 1:3096182/1‑62 (MQ=255) ggTAATGATACGGCGACCACCGCCTGTCTTC‑TGAATAATCCCCGGACGACCCTCTTTCGGGGACa > 1:2216096/1‑65 (MQ=255) ggTAATGAAACCGCCACCACCGCCTGTCTTC‑TGAATAATCCCCGGACGCCCCTCTTTCGGGGCCa > 1:1806913/1‑65 (MQ=255) ggTAATGAAACCGCCACCACCGCCTGTCTTC‑TGAATAATCCCCGGACGACCCTCTTTCGGGGCCa > 1:3505604/1‑65 (MQ=255) ggTAATGAAACCGCCACCACCGCCTGTCTTC‑TGAATAATCCCCGGACGACCCTCTTTCGGGGCCa > 1:2988880/1‑65 (MQ=255) gaaaTAAAACCGCCACCCCCGCCTGTCTTC‑TGAATAATCCCCGGACGACCCTCTTTCGGGGCCat < 1:3256443/63‑1 (MQ=255) gTAATGAACCCGCCACCCCCGCCTGTCTGT‑TGAATAATCCCCGGACGACCCTCTTTCGGGGCCat < 1:2625449/65‑1 (MQ=255) gTAATGAACCCGCCACCACCGCCTGTCTTC‑TGAATAATCCCCGGACGACCCTCTTTCGGGGCCat < 1:3782471/65‑1 (MQ=255) gTAATGAAACCGCCACCACCGCCTGTCTTC‑TGAATAATCCCCGGACGACCCTCTTTCGGGGCCat < 1:3144837/65‑1 (MQ=255) gTAATGAAACCGCCACCACCGCCTGTCTTC‑TGAATAATCCCCGGACGACCCTCTTTCGGGGCCat > 1:592178/1‑65 (MQ=255) atAACGAAACCGCCACCACCGCCTGTCTTC‑TGAATAATCCCCGGACGACCCTCTTTCGGGGCCat < 1:453745/64‑1 (MQ=255) tAATGAACCCTCCCCCCCCGCCTGTCTTC‑TGAATAATCCCCGGACGACCCTCTTTCGGGGCCata < 1:198230/65‑1 (MQ=255) aTGAAACCGCCACCACCGCCTGTCTTC‑TGAATAATCCCCGGACGACCCTCTTTCGGGGCCatata > 1:2602530/1‑65 (MQ=255) ggAACCCGCCGCCACCGCCTGTCTTC‑TGAATAATCCCCGGACGACCCTCTTTCGGGGCCatatat < 1:3644094/64‑1 (MQ=255) aacccGCCCCCGCCGCCTGTCTTC‑TGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATAc < 1:2432473/62‑1 (MQ=255) aaaCCGCCACCACCGCCTGTCTTC‑TGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATAc > 1:1202976/1‑65 (MQ=255) aCCGCCACCACCGCCTGTCTTC‑TGAATAATCCCCGGACGACCCTCTTTCGGCGCCATATATAccc > 1:1758780/1‑65 (MQ=255) | GACCACCACAAACGCGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCTGTCTTC‑TGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATACCC > NC_000913/395232‑395353 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |