Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,390,754	G→T	A252A (GCC→GCA)	rsgA ←	ribosome small subunit‑dependent GTPase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,390,754	0	G	T	100.0%	104.5 / NA	29	A252A (GCC→GCA)	rsgA	ribosome small subunit‑dependent GTPase A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (18/11);  total (18/11)

TCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTC  >  NC_000913/4390695‑4390813                                                            |                                                            tCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGt                                                        <  1:3805900/65‑1 (MQ=255) tCACGCACTCCGGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGt                                                        <  1:2138457/65‑1 (MQ=255)    cGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCgt                                                     <  1:1270954/65‑1 (MQ=255)    cGCACCCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCgt                                                     >  1:2743845/1‑65 (MQ=255)     gCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCgtg                                                    >  1:1372253/1‑65 (MQ=255)       aCTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCgtggt                                                  >  1:1364271/1‑65 (MQ=255)        cTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCgtggtg                                                 <  1:3401046/65‑1 (MQ=255)        agccTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCgtggtg                                                 <  1:813093/63‑1 (MQ=255)         tCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGgtgt                                                >  1:1277128/1‑65 (MQ=255)         tCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGgtgt                                                <  1:3647574/65‑1 (MQ=255)         gccTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGgtgt                                                <  1:1097483/64‑1 (MQ=255)             ggggAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTg                                            >  1:3550920/1‑65 (MQ=255)              gggAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGa                                           <  1:3515986/65‑1 (MQ=255)                 aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                        >  1:1359003/1‑65 (MQ=255)                 aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                        >  1:2262081/1‑65 (MQ=255)                 aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                        >  1:2397286/1‑65 (MQ=255)                 aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                        >  1:3990147/1‑65 (MQ=255)                 aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                        >  1:3199655/1‑65 (MQ=255)                 aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGGGTGCTGACCg                                        >  1:350135/1‑65 (MQ=255)                   tCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAg                                      <  1:477821/65‑1 (MQ=255)                      atcacatcacCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGccc                                   >  1:3813021/1‑65 (MQ=255)                       tcacatcacCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGcccc                                  >  1:1081754/1‑65 (MQ=255)                       tcacatcacCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGcccc                                  >  1:3644821/1‑65 (MQ=255)                         acatcacCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGa                                >  1:2072433/1‑65 (MQ=255)                                 gccgTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCag                        >  1:1564431/1‑65 (MQ=255)                                 gccgTGCGGGAAGCGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCag                        >  1:3204706/1‑65 (MQ=255)                                  ccgTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCaga                       <  1:2057588/65‑1 (MQ=255)                                   cgTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCagag                      <  1:2679922/65‑1 (MQ=255)                                      gCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGata                   >  1:2363813/1‑65 (MQ=255)                                            aGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTg             >  1:2247967/1‑65 (MQ=255)                                                  aCAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAgg       >  1:2030088/1‑65 (MQ=255)                                                  aCAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAgg       <  1:2599386/65‑1 (MQ=255)                                                     gCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAtc    >  1:4045103/1‑65 (MQ=255)                                                       cGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGCCAGAGATATCGTTGGTCAGGAtctc  <  1:2955209/65‑1 (MQ=255)                                                            |                                                            TCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTC  >  NC_000913/4390695‑4390813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑