Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,619,399	C→T	R94C (CGC→TGC)	marR →	DNA‑binding transcriptional repressor MarR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,619,399	0	C	T	100.0%	96.0 / NA	29	R94C (CGC→TGC)	marR	DNA‑binding transcriptional repressor MarR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/15);  total (14/15)

TATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAAT  >  NC_000913/1619338‑1619459                                                              |                                                             tATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGtgcg                                                           <  1:2141341/65‑1 (MQ=255)  aTGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTgcgg                                                          >  1:377029/1‑65 (MQ=255)   tGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTgcggc                                                         <  1:540448/65‑1 (MQ=255)   tGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTgcggc                                                         <  1:1876099/65‑1 (MQ=255)   tGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTgcggc                                                         <  1:2345660/65‑1 (MQ=255)    gCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTgcgggg                                                        >  1:1411607/1‑63 (MQ=255)    gCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTgcggcg                                                        >  1:3195814/1‑65 (MQ=255)    gCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTgcggcg                                                        >  1:521345/1‑65 (MQ=255)    gCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTgcggcg                                                        >  1:1899334/1‑65 (MQ=255)    gCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTgcggcg                                                        >  1:1966990/1‑65 (MQ=255)     cTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGt                                                       <  1:1354328/65‑1 (MQ=255)         aTCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTg                                                   >  1:1090025/1‑65 (MQ=255)         aTCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTg                                                   <  1:3142656/65‑1 (MQ=255)         aTCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTg                                                   >  1:2579209/1‑65 (MQ=255)         aTCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTg                                                   <  1:2562048/65‑1 (MQ=255)         aTCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTg                                                   <  1:3808531/65‑1 (MQ=255)         aTCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTg                                                   <  1:1557914/65‑1 (MQ=255)         aTCGCCTGGTCTGTAAAGGCTGGGGGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTg                                                   >  1:993547/1‑65 (MQ=255)         aTCGCCTGGTCTGTAAAGGCTGGGGGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTg                                                   >  1:3066257/1‑65 (MQ=255)         aTCGCCTGGTCTGTAAAGGCTGGGAGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCCTACTg                                                   >  1:957154/1‑65 (MQ=255)                ggTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAAc                                            >  1:1141024/1‑65 (MQ=255)                ggTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAAc                                            <  1:1144661/65‑1 (MQ=255)                  tCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACtt                                          >  1:2837109/1‑65 (MQ=255)                       aaaGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGGGCGGCGTACTGGTAAAACTTaccac                                     >  1:1944673/1‑65 (MQ=255)                          ggCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACcgg                                  >  1:3624712/1‑65 (MQ=255)                          ggCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACcgg                                  >  1:4015844/1‑65 (MQ=255)                          ggCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACcgg                                  >  1:1875974/1‑65 (MQ=255)                          ggCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGCCGTACTGGTAAAACTTACCACcgg                                  >  1:202422/1‑65 (MQ=255)                              gggTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCggcggc                              <  1:2548331/65‑1 (MQ=255)                                  ggAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGgcggcgcgg                          <  1:2720281/65‑1 (MQ=255)                                  ggAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGgcggcgcgg                          <  1:1469737/65‑1 (MQ=255)                                  ggAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGgcggcgcgg                          <  1:765498/65‑1 (MQ=255)                                       ggTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAata                     >  1:172729/1‑65 (MQ=255)                                       ggTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAata                     >  1:720755/1‑65 (MQ=255)                                       ggTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAata                     >  1:3173926/1‑65 (MQ=255)                                            ccGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGa                <  1:671674/65‑1 (MQ=255)                                            ccGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGa                <  1:3427036/65‑1 (MQ=255)                                                 cccGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAAt           >  1:659646/1‑65 (MQ=255)                                                        gACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCa    <  1:690977/65‑1 (MQ=255)                                                          cAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCCCCAAt  >  1:452993/1‑65 (MQ=255)                                                          cAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAAt  <  1:43105/65‑1 (MQ=255)                                                              |                                                             TATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAAT  >  NC_000913/1619338‑1619459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑