| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,414,906 | 0 | T | G | 35.3% | 17.4 / 13.4 | 17 | V211G (GTG→GGG) | aidB | putative acyl‑CoA dehydrogenase AidB |
| Reads supporting (aligned to +/- strand): ref base T (0/11); new base G (6/0); total (6/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.08e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGTTCCGATGTTATGAGCAACACCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCTGGTGGGGCAT‑AAATGGTTTTTCTCGGTTCCGCAAAGCGATGCGCATCTGGTGCTGGCGCAGACCGCG > NC_000913/4414842‑4414970 | ggTTCCGATGTTATGAGCAACACCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCtggt < 1:1767183/65‑1 (MQ=255) ccGATGTTATGAGCAACACCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCTGGTgggg < 1:2386799/65‑1 (MQ=255) gCAACACCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCTGGGGGGGGGT‑AAATGGttt > 1:643268/1‑65 (MQ=255) gCAACACCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCTGGGGGGGGGT‑AAAAGGgtt > 1:875337/1‑65 (MQ=255) gCAACACCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCTGGGGGGGGCT‑AAATGGgtt > 1:744465/1‑65 (MQ=255) gCAACACCACCCGTGCAGAGCGTCTGGAAGATGGCTCATATCGGCTGGGGGGGGAT‑AAAAgggcg > 1:342971/1‑62 (MQ=255) accaccCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCTGGGGGGGGGTGAAAGGGttttttt > 1:1804272/1‑63 (MQ=255) ccGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCTGGGGGGGGGT‑AAAAGGTTTTTCTCTGttt > 1:2466853/1‑64 (MQ=255) cGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCTGGTGGGGCAT‑AAATGGTTTTTCTCGGTTcc < 1:2627531/65‑1 (MQ=255) agCGTCTGGAAGATGGCTCTTATCGGCTGGTGGGGCAT‑AAATGGTTTTTCTCGGTTCCGCAAAGc < 1:884648/65‑1 (MQ=255) gTCTGGAAGATGGCTCTTATCGGCTGGTGGGGCAT‑AAATGGTTTTTCTCGGTTCCGCAAAGCGAt < 1:1490245/65‑1 (MQ=255) gAAGATGGCTCTTATCGGCTGGTGGGGCAT‑AAATGGTTTTTCTCGGTTCCGCAAAGCGATGCGCa < 1:312364/65‑1 (MQ=255) gAAGATGGCTCTTATCGGCTGGTGGGGCAT‑AAATGGTTTTTCTCGGTTCCGCAAAGCGATGCGCa < 1:688624/65‑1 (MQ=255) gAAGATGGCTCTTATCGGCTGGTGGGGCAT‑AAATGGTGTTTCTCGGTTCCGCAAAGCGATGCGCa < 1:85973/65‑1 (MQ=255) gAAGATGGCTCTTATCGAGTGGTGGGGCAT‑AAATGGTTTTTCTCGGTTCCGCAAAGCGATGCGCa < 1:1771440/65‑1 (MQ=255) gAAGATGGCTCGTATCGGCTGGTGGGGCAT‑AAATGGTTTTTCTCGGTTCCGCAAAGCGATGCGCa < 1:321982/65‑1 (MQ=255) aaGATGGCTCTTATCGGCTGGTGGGGCAT‑AAATGGTTTTTCTCGGTTCCGCAAAGCGATGCGCAt < 1:2256031/65‑1 (MQ=255) aTGGCTCTTATCGGCTGGTGGGGCAT‑AAATGGTTTTTCTCGGTTCCGCAAAGCGATGCGCATCTg < 1:1908064/65‑1 (MQ=255) ggCTCTTATCGGCTGGTGGGGTAT‑AAATGGTTTTTCTCGGTTCCGCAAAGCGATGCGCATCTGGt < 1:3074475/65‑1 (MQ=255) tctTATCGGCTGGGGGGGGAT‑AAATGGGTTTTTTTGGTTTCGCAAAACGATGCGCCTCTGGGGCt > 1:2473937/1‑65 (MQ=255) gCTGGTGGGGCAT‑AAATGGTTTTTCTCGGTTCCGCAAAGCGATGCGCATCTGGTGCTGGCGCAGa < 1:209146/65‑1 (MQ=255) tggGGCAT‑AAATGGTTTTTCTCGGTTCCGCAAAGCGATGCGCATCTGGTGCTGGCGCAGACcgcg < 1:1151560/65‑1 (MQ=255) | GGTTCCGATGTTATGAGCAACACCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCTGGTGGGGCAT‑AAATGGTTTTTCTCGGTTCCGCAAAGCGATGCGCATCTGGTGCTGGCGCAGACCGCG > NC_000913/4414842‑4414970 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |