Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,390,754	G→T	A252A (GCC→GCA)	rsgA ←	ribosome small subunit‑dependent GTPase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,390,754	0	G	T	100.0%	83.6 / NA	23	A252A (GCC→GCA)	rsgA	ribosome small subunit‑dependent GTPase A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (13/10);  total (13/10)

CTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTC  >  NC_000913/4390694‑4390813                                                             |                                                            cTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGctgcgg                                                         >  1:1095521/1‑65 (MQ=255)  tCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGt                                                        <  1:2003718/65‑1 (MQ=255)          tCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGgtgt                                                <  1:826100/65‑1 (MQ=255)          tCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGgtgt                                                <  1:1220914/65‑1 (MQ=255)          tCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGgtgt                                                <  1:2533560/65‑1 (MQ=255)          tCCGGGGGAGTCAGTCACATCCCCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCgtggtct                                                <  1:1234654/65‑3 (MQ=255)            ttGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGtgtgt                                              >  1:2211977/2‑64 (MQ=255)                ggAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGAc                                          >  1:2377081/1‑65 (MQ=255)                ggAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGAc                                          >  1:2211851/1‑65 (MQ=255)                  aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGCGCTGCGGTCGTGGTGTGCTGACCg                                        >  1:2804219/1‑65 (MQ=255)                  aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGNcag                                        >  1:2147966/1‑63 (MQ=255)                  aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                        >  1:1090313/1‑65 (MQ=255)                  aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                        >  1:712364/1‑65 (MQ=255)                  aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                        >  1:667628/1‑65 (MQ=255)                  aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                        >  1:369046/1‑65 (MQ=255)                  aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                        >  1:2634575/1‑65 (MQ=255)                  aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                        >  1:1617832/1‑65 (MQ=255)                  aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                        >  1:2016286/1‑65 (MQ=255)                  aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGCGGTGCGCTGACCg                                        >  1:2102344/1‑65 (MQ=255)                    gcAACCACACCCCCGCCGGGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAg                                      <  1:2138441/64‑1 (MQ=255)                          acatcacCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGa                                >  1:1817407/1‑65 (MQ=255)                          acatcacCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGa                                >  1:782187/1‑65 (MQ=255)                            atcccCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGt                              <  1:931792/65‑1 (MQ=255)                                  gccgTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGGTGTCag                        >  1:964143/1‑65 (MQ=255)                                   ccgTGCGGGAGGCGATACAGCCGAGCTGCGGTCGTCCTGTGCTGACCGAGTCCCGAGTTGTCaga                       <  1:1976256/65‑1 (MQ=255)                                   ccgTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCaga                       <  1:2664615/65‑1 (MQ=255)                                    cgTGGGGGAAGTGATGCAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCagag                      <  1:1567533/65‑1 (MQ=255)                                            aaGTGATACAGCCGAGCTGCGGTCGTGGTGTGCGGACCGAGCCCCGAGTTGTCAGAGATATCGtt              <  1:455888/65‑1 (MQ=255)                                             aGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTg             >  1:851155/1‑65 (MQ=255)                                                   aCAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAgg       <  1:2017823/65‑1 (MQ=255)                                                        cGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAtctc  >  1:2713279/1‑65 (MQ=255)                                                        cGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAtctc  <  1:244424/65‑1 (MQ=255)                                                        cGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAtctc  <  1:106747/65‑1 (MQ=255)                                                             |                                                            CTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTC  >  NC_000913/4390694‑4390813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑