Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	481,404	C→A	Q1000H (CAG→CAT)	acrB ←	multidrug efflux pump RND permease AcrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	481,404	0	C	A	100.0%	128.2 / NA	34	Q1000H (CAG→CAT)	acrB	multidrug efflux pump RND permease AcrB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (20/14);  total (20/14)

ATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCA  >  NC_000913/481348‑481465                                                         |                                                              aTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTATGCGCGcc                                                       <  1:1986889/65‑1 (MQ=255)        gTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTATGCGCGCCGGAACCa                                                <  1:3193077/65‑1 (MQ=255)             gTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTATGCGCGCCGGAACCAGCAcc                                           <  1:1859235/65‑1 (MQ=255)              ttGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTATGCGCGCCGGAACCAGCACCa                                          <  1:2776948/65‑1 (MQ=255)               tGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTATGCGCGCCGGAACCAGCACCAg                                         >  1:278486/1‑65 (MQ=255)               tGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTATGCGCGCCGGAACCAGCACCAg                                         <  1:2315517/65‑1 (MQ=255)               tGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTATGCGCGCCGGAACCAGCACCAg                                         >  1:1703973/1‑65 (MQ=255)                   gTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTATGCGCGCCGGAACCAGCACCAGTACt                                     <  1:2649474/65‑1 (MQ=255)                       ccATCCCGCCCATTACACCGGTACCTACTGCGTTATGCGCGCCGGAACCAGCACCAGTACTGATa                                 >  1:840454/1‑65 (MQ=255)                                ccATTACACCGGTACCTACTGCGTTATGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGc                        <  1:920017/65‑1 (MQ=255)                                ccATTACACCGGTACCTACTGCGTTATGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGc                        <  1:459364/65‑1 (MQ=255)                                ccATTACACCGGTACCTACTGCGTTATGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGc                        <  1:2091710/65‑1 (MQ=255)                                ccATTACACCGGTACCTACTGCGTTATGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGc                        <  1:141775/65‑1 (MQ=255)                                        ccGGTACCTACTGCGTTATGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGcc                >  1:1472722/1‑65 (MQ=255)                                          ggTACCTACTGCGTTATGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGa              >  1:1849514/1‑65 (MQ=255)                                            tACCTACTGCGTTATGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAgg            <  1:905031/65‑1 (MQ=255)                                            tACCTACTGCGTTATGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAgg            <  1:1308447/65‑1 (MQ=255)                                            tACCTACTGCGTTATGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAgg            <  1:1623829/65‑1 (MQ=255)                                             aCCTACTGCGTTATGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGa           >  1:695977/1‑65 (MQ=255)                                             aCCTACTGCGTTATGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGa           <  1:245289/65‑1 (MQ=255)                                             aCCTACTGCGTTATGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGa           >  1:2472425/1‑65 (MQ=255)                                             aCCTACTGCGTTATGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAAAGCCGAGGa           >  1:2360253/1‑65 (MQ=255)                                               cTACTGCGTTATGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATa         >  1:1002971/1‑65 (MQ=255)                                               cTACTGCGTTATGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATa         >  1:1572926/1‑65 (MQ=255)                                               cTACTGCGTTATGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATa         >  1:2251550/1‑65 (MQ=255)                                               cTACTGCGTTATGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATa         >  1:1629463/1‑65 (MQ=255)                                               cTACTGCGTTATGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATa         >  1:1633895/1‑65 (MQ=255)                                               cTACTGCGTTATGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGACATAACGCCGAGGATa         >  1:2961090/1‑65 (MQ=255)                                                 aCTGCGTTATGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATaaa       >  1:2836666/1‑65 (MQ=255)                                                  cTGCGTTAGGCGCGCGGGAGCCAGCACCAGTGCTGATAACCAGCGGCATAACGCCGAGGATAAAc      <  1:818343/65‑1 (MQ=25)                                                   tGCGTTATGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACg     <  1:2100976/65‑1 (MQ=255)                                                     cGTTATGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGcc   >  1:1132165/1‑65 (MQ=255)                                                      gTTATGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCa  >  1:2365191/1‑65 (MQ=255)                                                      gTTATGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCa  >  1:3167396/1‑65 (MQ=255)                                                      gTTATGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCa  >  1:2235844/1‑65 (MQ=255)                                                      gTTATGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCa  >  1:1226108/1‑65 (MQ=255)                                                      gTTATGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCa  >  1:709818/1‑65 (MQ=255)                                                         |                                                              ATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCA  >  NC_000913/481348‑481465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑