Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	883,661	C→T	intergenic (‑273/‑12)	ybjG ← / → mdfA	undecaprenyl pyrophosphate phosphatase/multidrug efflux pump MdfA/Na(+):H(+) antiporter/K(+):H(+) antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	883,661	0	C	T	100.0%	159.2 / NA	45	intergenic (‑273/‑12)	ybjG/mdfA	undecaprenyl pyrophosphate phosphatase/multidrug efflux pump MdfA/Na(+):H(+) antiporter/K(+):H(+) antiporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/20);  total (25/20)

GCTACAAAATTCGCTTTCTCGTTAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCGAAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTAC  >  NC_000913/883600‑883721                                                              |                                                             gCTACAAAATTCGCTTTCTCGTTAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGTgaa                                                           >  1:51014/1‑65 (MQ=255)  cTACAAAATTCGCTTTCTCGTTAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGTgaag                                                          <  1:760003/65‑1 (MQ=255)   tACAAAATTCGCTTTCTCGTTAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGTgaaga                                                         <  1:49614/65‑1 (MQ=255)   tACAAAATTCGCTTTCTCGTTAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGTgaaga                                                         <  1:462592/65‑1 (MQ=255)                ttCTCGTTAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGTGAAGAAATTGGATGCaaa                                            <  1:552435/65‑1 (MQ=255)                ttCTCGTTAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGTGAAGAAATTGCATGCaaa                                            <  1:2098027/65‑1 (MQ=255)                   tcGTTAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGTGAAGAAATTGCATGCAaaata                                         <  1:1234996/65‑1 (MQ=255)                         gCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGTGAAGCAATTGCATGCAAAATAAATTAg                                   >  1:2104940/1‑65 (MQ=255)                         gCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGTGAAGAAATTGCATGCAAAATAAATTAg                                   >  1:2348298/1‑65 (MQ=255)                         gCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGTGAAGAAATTGCATGCAAAATAAATTAg                                   >  1:2049858/1‑65 (MQ=255)                         gCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGTGAAGAAATTGCATGCAAAATAAATTAg                                   >  1:2406174/1‑65 (MQ=255)                         gCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGTGAAGAAATTGCATGCAAAATAAATTAg                                   >  1:1971591/1‑65 (MQ=255)                         gCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGTGAAGAAATTGCATGCAAAATAAATTAg                                   >  1:275796/1‑65 (MQ=255)                         gCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGTGAAGAAATTGCATGCAAAATAAATTAg                                   >  1:938191/1‑65 (MQ=255)                         gCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGTGAAGAAATTGCATGCAAAATAAATTAg                                   >  1:1171418/1‑65 (MQ=255)                           tGCGCTTTTATTAAACTCTGCGCGATTATTATTGGTGAAGAAATTGCATGCAAAATAAATTAGCt                                 >  1:2802612/1‑65 (MQ=255)                           tGCGCTTTTATTAAACTCTGCGCGATTATTATTGGTGAAGAAATTGCATGCAAAATAAATTAGCt                                 >  1:1415442/1‑65 (MQ=255)                              gcTTTTATTAAACTCTGCGCGATTATTATTGGTGAAGAAATTGCATGCAAAATAAATTAGCTTcc                              <  1:1856470/65‑1 (MQ=255)                                 tttATTAAACTCTGCGCGATTATTATTGGTGAAGAAATTGCATGCAAAATAAATTAGCTTCCGGt                           <  1:2166948/65‑1 (MQ=255)                                 gttattaAACTCTGCGCGATTATTATTGGTGAAGAAATTGCATGCAAAATAAATTAGCTTCCGGt                           <  1:1588144/64‑1 (MQ=255)                                  ttattaAACTCTGCGCGATTATTATTGGTGAAGAAATTGCATGCAAAATAAATTAGCTTCCGGTg                          <  1:1192090/65‑1 (MQ=255)                                    attaAACTCTGCGCGATTATTATTGGTGAAGAAATTGCATGCAAAATAAGTTAGCTTCCGGTGcc                        >  1:3281495/1‑65 (MQ=255)                                        aaCTCTGCGCGATTATTATTGGTGAAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGc                    >  1:17635/1‑65 (MQ=255)                                        aaCTCTGCGCGATTATTATTGGTGAAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGc                    <  1:2540101/65‑1 (MQ=255)                                        aaCTCTGCGCGATTATTATTGGTGAAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGc                    <  1:2600810/65‑1 (MQ=255)                                         aCTCTGCGCGATTATTATTGGTGAAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCGGGCt                   >  1:1056912/1‑65 (MQ=255)                                         aCTCTGCGCGATTATTATTGGTGAAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCt                   >  1:2146750/1‑65 (MQ=255)                                           tctGCGCGATTATTATTGGTGAAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTg                 >  1:1035197/1‑65 (MQ=255)                                           tctGCGCGATTATTATTGGTGAAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTg                 >  1:2309079/1‑65 (MQ=255)                                           tctGCGCGATTATTATTGGTGAAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTg                 >  1:1194040/1‑65 (MQ=255)                                           tctGCGCGATTATTATTGGTGAAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTg                 >  1:2644435/1‑65 (MQ=255)                                           tctGCGCGATTATTATTGGTGAAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTg                 >  1:1302801/1‑65 (MQ=255)                                           tctGCGCGATTATTATTGGTGAAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTg                 >  1:656405/1‑65 (MQ=255)                                           tctGCGCGATTATTATTGGTGAAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTg                 >  1:1792051/1‑65 (MQ=255)                                           tctGCGCGATTATTATTGGTGAAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTg                 >  1:1554983/1‑65 (MQ=255)                                           tctGCGCGATTATTATTGGTGAAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTg                 >  1:171159/1‑65 (MQ=255)                                           tctGCGCGATTATTATTAGTGAAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTg                 >  1:1333855/1‑65 (MQ=255)                                             tGCGGGATGATGATTGGTGAAGAAATTGCATGCAAAATGAATGAGCTTCCGGTGCCAGGCTTGGa               <  1:3026261/65‑1 (MQ=255)                                             tGCGCGATTATTATTGGTGAAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGa               <  1:96773/65‑1 (MQ=255)                                             tGCGCGATTATTATTGGTGAAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGa               <  1:1781406/65‑1 (MQ=255)                                             tGCGCGATTATTATTGGTGAAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGa               <  1:2894403/65‑1 (MQ=255)                                             tGCGCGATTATTATTGGTGAAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGa               <  1:2084827/65‑1 (MQ=255)                                               cgcgATTATTATTGGTGAAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGatg             >  1:705307/1‑63 (MQ=255)                                                gcgATTATTATTGGTGAAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGt            >  1:1984487/1‑65 (MQ=255)                                                   attattattGGTGAAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAg         >  1:1867295/1‑65 (MQ=255)                                                     tattattGGTGAAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGc       <  1:288939/65‑1 (MQ=255)                                                     tattattGGTGAAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGc       <  1:1332634/65‑1 (MQ=255)                                                        tattGGTAAAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGtt    <  1:857434/65‑1 (MQ=255)                                                          ttGGTGAAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTAc  <  1:2675225/65‑1 (MQ=255)                                                              |                                                             GCTACAAAATTCGCTTTCTCGTTAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCGAAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTAC  >  NC_000913/883600‑883721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑