Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,619,370	Δ1 bp	coding (251/435 nt)	marR →	DNA‑binding transcriptional repressor MarR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,619,370	0	T	.	100.0%	110.0 / NA	23	coding (251/435 nt)	marR	DNA‑binding transcriptional repressor MarR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (15/8);  total (15/8)

ATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGC  >  NC_000913/1619308‑1619431                                                               |                                                              aTTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGG‑GGAAAg                                                         <  1:3416477/68‑1 (MQ=255) aTTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGG‑GGAAAg                                                         <  1:2915773/68‑1 (MQ=255)         tcgACCTGGGAGCACTGACCCGTATGCAGGATCGCCTGGTCTGTAAAGGCTGGG‑GGAAAGGTTGCCGa                                                 <  1:3401292/68‑1 (MQ=255)                gggAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGG‑GGAAAGGTTGCCGAACCCGaa                                          <  1:2746045/68‑1 (MQ=255)                  gAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGG‑GGAAAGGTTGCCGAACCCGAATg                                        >  1:2739963/1‑68 (MQ=255)                   aGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGG‑GGAAAGGTTGCCGAACCCGAATGa                                       >  1:3739536/1‑68 (MQ=255)                   aGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGG‑GGAAAGGTTGCCGAACCCGAATGa                                       <  1:429261/68‑1 (MQ=255)                   aGCACTGACCCGTAAGCTGGATTGCCTGGTCTGTAAAGGCTGGG‑GGAAAGGTTGCCGAACCAGAATGa                                       >  1:3147455/1‑68 (MQ=255)                    gCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGG‑GGAAAGGTTGCCGAACCCGAATGAc                                      >  1:1742776/1‑68 (MQ=255)                    gCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGG‑GGAAAGGTTGCCGAACCCGAATGAc                                      >  1:3081447/1‑68 (MQ=255)                      aCTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGG‑GGAAAGGTTGCCGAACCCGAATGACaa                                    >  1:825634/1‑68 (MQ=255)                      aCTGACCCGTATGCTGGATCGCCTGGTCCGTAAAGGCTGGG‑GGCAAGGCTGCCGAACCCGAAACACaa                                    >  1:2307236/1‑68 (MQ=255)                        tGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGG‑GGAAAGGTTGCCGATCCCGAATGACAAgc                                  <  1:3166629/68‑1 (MQ=255)                           cccGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGG‑GGAAAGGTTGCCGAACCCGAATGACAAGcgcg                               >  1:2291932/1‑68 (MQ=255)                                aTGCTGGATCGCCTGGTCTGTAAAGGCTGGG‑GGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTa                          >  1:1229532/1‑68 (MQ=255)                                  gCTGGATCGCCTGGTCTGTAAAGGCTGGG‑GGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACt                        >  1:2731627/1‑68 (MQ=255)                                  gCTGGATCGCCTGGTCTGTAAAGGCTGGG‑GGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACt                        >  1:3846644/1‑68 (MQ=255)                                   cTGGATCGCCTGGTCTGTAAAGGCTGGG‑GGAAAGGTTGCCGAACCCGAATGGCAAGCGCGGCGTACTg                       <  1:1784124/68‑1 (MQ=255)                                   cTGGATCGCCTGGTCTGTAAAGGCTGGG‑GGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTg                       <  1:1306158/68‑1 (MQ=255)                                   cTGGATCGCCTGGTCTCTAAAGGCTGGG‑GGAAAGTTTGCCGAACCCGAATGACAAGCGCGGCGTACTg                       <  1:3476619/68‑1 (MQ=255)                                       aTCGCCTGGTCTGTAAAGGCTGGG‑GGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTaa                   >  1:3637775/1‑68 (MQ=255)                                       aTCGCCTGGTCTGTAAAGGCTGGG‑GGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTaa                   >  1:1718781/1‑68 (MQ=255)                                              ggTCTGTAAAGGCTGGG‑GGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTac            >  1:978049/1‑68 (MQ=255)                                                tCTGTAAAGGCTGGG‑GGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTacca          >  1:626572/1‑68 (MQ=255)                                                     aaaGGCTGGG‑GGAAAGGTTGCCGAACTCCCCTAACCAGCCCGGCCTCCTGGTACAACCTaccacctgt     >  1:437688/1‑65 (MQ=255)                                                        ggCTGGG‑GGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCggcggc  >  1:1976363/1‑68 (MQ=255)                                                        ggCTGGG‑GGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCggcggc  >  1:997087/1‑68 (MQ=255)                                                               |                                                              ATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGC  >  NC_000913/1619308‑1619431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑