| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,563,481 | 0 | T | G | 35.0% | 56.3 / 34.1 | 40 | V490G (GTG→GGG) | glpD | aerobic glycerol 3‑phosphate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (0/26); new base G (14/0); total (14/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.31e-11 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATA‑CGCAGCAGAGGTTATCGCTGGC‑GTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTC > NC_000913/3563416‑3563547 | gCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCtggtgg < 1:22120/68‑1 (MQ=255)gCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCtggtgg < 1:541062/68‑1 (MQ=255) gTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCCGTGGCTGGTGGAg < 1:3936387/68‑1 (MQ=255) gTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCtggggggg > 1:2562737/1‑66 (MQ=255) gTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAg < 1:204592/68‑1 (MQ=255) tCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGt < 1:116882/68‑1 (MQ=255) gCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGtat < 1:1292964/68‑1 (MQ=255) acaAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATA‑c < 1:1501265/68‑1 (MQ=255) acaAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGGATA‑c > 1:2474518/1‑68 (MQ=255) aCAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATA‑Cgcagc < 1:617132/68‑1 (MQ=255) aCAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATA‑Cgcagc < 1:2128144/68‑1 (MQ=255) aCAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGGATA‑Cgcagg > 1:2809444/1‑67 (MQ=255) aCAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGGATA‑Cgcagc > 1:1408782/1‑68 (MQ=255) gCATGTGGCTAAATGCGGATCAACAATCTCGTTTGAGTCAGTGGCTGGTGGAGTATA‑CGCAGCAGAgg < 1:2765255/68‑1 (MQ=255) gCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATA‑CGCAGCAGAgg < 1:2992016/68‑1 (MQ=255) gCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATA‑CGCAGCAGAgg < 1:1897088/68‑1 (MQ=255) gCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATA‑CGCAGCAGAgg < 1:683339/68‑1 (MQ=255) gCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATA‑CGCAGCAGAgg < 1:440423/68‑1 (MQ=255) gCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGGGTA‑CGCCGCAgggg > 1:568635/1‑68 (MQ=255) gCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGGATA‑CGCAGCGGAgg > 1:1586384/1‑68 (MQ=255) gCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGGATA‑AGCCGCAgggg > 1:1365159/1‑68 (MQ=255) gCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGGATA‑AGCCGCAGAgg > 1:1935073/1‑68 (MQ=255) cATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATA‑CGCAGCAGAGGt < 1:2291920/68‑1 (MQ=255) cATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATA‑CGCAGCAGAGGt < 1:3169958/68‑1 (MQ=255) cATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGGATAGCGC‑GCAGAggg > 1:2905062/1‑67 (MQ=255) aTGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGGTTA‑CGGAGCAGAgggt > 1:47009/1‑66 (MQ=255) gCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGGATA‑AGCAGCAGAGGNGAttcc > 1:2607066/1‑65 (MQ=255) tGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGTATA‑CGGCGCAGAGGGTATTGCTGGc‑gg > 1:3895619/1‑67 (MQ=255) tGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGGATA‑CGCAGCCGAGGGTATtggtggggg > 1:3173782/1‑65 (MQ=255) gCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATA‑CGCAGCAGAGGTTATCGCTGGc‑gtc < 1:2968429/68‑1 (MQ=255) caaTCTCGTGTGAGTCAGTGGCTGGTGGAGTATA‑CGCAGCAGAGGTTATCGCTGGC‑GTCGTAAATTaa < 1:964486/68‑1 (MQ=255) tctcGTGTGAGTCAGTGGCTGGGGGGGGATA‑CGCCGCAGGGGGTATCGCCGGC‑GGCGGAAAATAACgg > 1:133653/1‑67 (MQ=255) ctcGTGTGAGTCAGTGGCTGGGGGGGGATA‑CGCAGCAGAGGGTTTCCCTGGCGGCCGAAAATTaagca > 1:1489451/1‑65 (MQ=255) tgtgAGTCAGTGGCTGGTGGAGTATA‑CGCAGCAGAGGTTATCGCTGGC‑GTCGTAAATTAACGTAAGGt < 1:1121613/68‑1 (MQ=255) tgtgAGTCAGTGGCTGGTGGAGTATA‑CGCAGCAGAGGTTATCGCTGGC‑GTCGTAAATTAACGTAAGGt < 1:740439/68‑1 (MQ=255) tgtgAGTCAGTGGCTGGTGGAGTATA‑CGCAGCAGAGGTTATCGCTGGC‑GTCGTAAATTAACGTAAGGt < 1:605551/68‑1 (MQ=255) tgtgAGTCAGTGGCTGGGGGGGGATA‑AGCAAGAGAGGGTATTCCTTGC‑GGCGTTAATTAACGGaaagc > 1:796672/1‑65 (MQ=255) gtgAGTCAGTGGCTGGGGGGGGATA‑CGCCGCAGAGGGTATCGCTGGG‑GGCGTAAATTAACGGAAgggg > 1:3852773/1‑66 (MQ=255) tgAGTCAGTGGCTGGTGGAGTATA‑CGCAGCAGAGGTTATCGCTGGC‑GTCGTAAATTAACGTAAGGTGa < 1:160695/68‑1 (MQ=255) cAGTGGCTGGTGGAGTATA‑CGCAGCAGAGGTTATCGCTGGC‑GTCGTAAATTAACGTAAGGTGATCAgg < 1:524532/68‑1 (MQ=255) cAGTGGCTGGTGGAGTATA‑CGCAGCAGAGGTTATCGCTGGC‑GTCGTAAATTAACGTAAGGTGATCAgg < 1:3238393/68‑1 (MQ=255) cAGTGGCTGGTGGAGTATA‑CGCAGCAGAGGTTATCGCTGGC‑GTCGTAAATTAACGTAAGGTGATCAgg < 1:232602/68‑1 (MQ=255) cAGTGGCTGGTGGAGTATA‑CGCAGCAGAGGTTATCGCTGGC‑GTCGTAAATTAACGTAAGGTGATCAgg < 1:342295/68‑1 (MQ=255) ggCTGGGGGCGTATC‑CGCAGCAGAGGGTATCCCTGGC‑GTCGTAAATTCACGTAAGGGGATCAAGGCAg > 1:3167525/1‑68 (MQ=255) gCTGGTGGAGTATA‑CGCAGCAGAGGTTATCGCTGGC‑GTCGTAAATTAACGTAAGGTGATCAGGTCAGa < 1:2358493/68‑1 (MQ=255) cTGGTGGAGTATA‑CGCAGCAGAGGTTATCGCTGGC‑GTCGTAAATTAACGTAAGGTGATCAGGTCAGAt < 1:3999128/68‑1 (MQ=255) tggtggAGTATA‑CGCAGCAGAGGTTATCGCTGGC‑GTCGTAAATTAACGTAAGGTGATCAGGTCAGAtt < 1:286253/68‑1 (MQ=255) gtggAGTATA‑CGCAGCAGAGGTTATCGCTGGC‑GTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTc > 1:1202863/1‑68 (MQ=255) | GCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATA‑CGCAGCAGAGGTTATCGCTGGC‑GTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTC > NC_000913/3563416‑3563547 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |