| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 296,504 | T→G | N198T (AAC→ACC) | intF ← | CP4‑6 prophage; putative phage integrase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 296,504 | 0 | T | G | 80.0% | 15.7 / 1.3 | 10 | N198T (AAC→ACC) | intF | CP4‑6 prophage; putative phage integrase |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base G (8/0); total (9/1) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.08e-02 | |||||||||||
ATTTTGCCTTTCTGTACTCAGCCACGCTGCTATGTAATCTGGGGTTAGCTCCGATAACGGCAGGTTGAGCAAACTAGCCAATGGTCCAGCCGAAGTCGGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACG > NC_000913/296440‑296571 | aTTTTGCCTTTCTGTACTCAGCCACGCTGCTATGTAATCTGGGGTTAGCTCCGATAACGGCAGGTTGa < 1:1153997/68‑1 (MQ=255) tttGCCTTTCTGTACTCAGCCACGCTGCTATGTAATCTGGGGTTAGCTCCGATAACGGCAGGGTGGGc > 1:3989730/1‑68 (MQ=255) tCAGCCACGCTGCTATGTAATCTGGGGTTAGCTCCGATAACGGCAGGGTGGGGAAACGAGACAATggg > 1:3414574/1‑67 (MQ=255) tCAGCCACGCTGCTATGTAATCTGGGGTTAGCTCCGATAACGGCAGGGTGGGGAAACCAGCCAATggg > 1:2941929/1‑67 (MQ=255) gCCACGCTGCTATGTAATCTGGGGTTAGCTCCGATAACGGCAGGGTGGGGGAACTAGACAAAGGTCCa > 1:1620588/1‑68 (MQ=255) gCCACGCTGCTATGTAATCTGGGGTTAGCTCCGATAACGGCAGGGTGGGCAAACTAGCCAATGGGccc > 1:2702691/1‑67 (MQ=255) cACGCTGCTATGTAATCTGGGGTTAGCTCCGATAACGGCAGGGTGGGCAAAATAGCCAAAGGGGCAGc > 1:2189810/1‑68 (MQ=255) gctgctATGTAATCTGGGGTTAGCTCCGATAACGGCAGGGTGGGGCAAAAAGCCAAAGGGCCCGccca > 1:3127572/1‑66 (MQ=255) gctgctATGTAATCTGGGGTTAGCTCCGATAACGGCAGGGTGGGGAAAATAACCAATGGGCCAGCCGa > 1:2064454/1‑68 (MQ=255) tATGTAATCTGGGGTTAGCTCCGATAACGGCAGGGTGGGGAAACTAGCCCAAGGGGCAGCCGCAgagg > 1:3427622/1‑65 (MQ=255) gaaaTCGGGGTTTACCCCCGATATCGGCAGGTTGACCAACCTAGCCAATGGTCCAGCCGAAGTCGGGc < 1:552793/66‑1 (MQ=255) gTATTCTGGGTTTGGCCCCAAAAACGGCGGGTGGAGAAAATTAGCCAATGGTCCAGCCGAAGTCGGGc < 1:2781311/68‑1 (MQ=255) gTAATCTGGGGTTAGCTCCGATAACGGCAGGGTGGGGAAACTAGCCAATGGGTCCGCCGGAGGCgggg > 1:1061931/1‑67 (MQ=255) gTAATCTGGGGTTAGCTCCGATAACGGCAGGGTGGGGAAAATAACCAAAGGTCCAGCCGAAGGCGGGc > 1:1558729/1‑68 (MQ=255) gTAATCTGGGGTTAGCTCCGATAACGGCAGGGTGGGCAAACTAACCAATGGTCCCGGCGGAGGCgggg > 1:809730/1‑67 (MQ=255) gTAATCTGGGGTTAGCTCCGATAACGGCAGGGTGAGGAAACTAGCCAATGGGCCAGCCGAAGGCGGGc > 1:306735/1‑68 (MQ=255) gTAATCTGGGGTTAGCTCCGATAACGGCAGGGTGAGCAAAATAGCCAATGGGCCAGCCGAAGTCGGGc > 1:2860778/1‑68 (MQ=255) aaTCTGGGGTTAGCTCCGATAACGGCAGGGTGGGGAAAAAAGCCAATGGGCCAGCCGGAGGCGGGGCt > 1:796012/1‑68 (MQ=255) aaTCTGGGGTTAGCTCCGATAACGGCAGGGTGGGCCAAACAGCCCATGGTGCAGCCGCAGTCGGGGCt > 1:546895/1‑68 (MQ=255) aaTCTGGGGTTAGCTCCGATAACGGCAGGGTGGGCAAACTAGCCCACGGTCCCCCCCCACTCGGGCCt > 1:1835633/1‑68 (MQ=255) aaTCTGGGGTTAGCTCCGATAACGGCAGGGTGGGCAAACAAACCAATGGGCCAGGCCCAGGCGGGcgt > 1:3140381/1‑66 (MQ=255) aaTCTGGGGTTAGCTCCGATAACGGCAGGGTGGGCAAAATAACCAATGCGGCAGCCGAAGGCGGGGCt > 1:3595917/1‑68 (MQ=255) aaTCTGGGGTTAGCTCCGATAACGGCAGGGTGGGCAAAACAGCCCAAGGGCCAGCCGCAGGCggggcc > 1:1993622/1‑65 (MQ=255) aaTCTGGGGTTAGCTCCGATAACGGCAGGGTGAGCAAACCAGCCAATGGGCCACCCGAAGGCGGGCCt > 1:2342944/1‑68 (MQ=255) aaTCTGGGGTTAGCTCCGATAACGGCAGGGGGAGGAAAATAGCCCATCGGCCAGCCCCAGGCGGGCCt > 1:550134/1‑68 (MQ=255) cTGGGGTTAGCTCCGATAACGGCAGGGTGGGGAAAATAGCCAATGGGCCAGCCGAAGGCGGGGCtttg > 1:2335003/1‑66 (MQ=255) ggTTAGCTCCGATAACGGCAGGGTGGGCAAAATAGCCAAAGGTGCAGCCGAAGACGGGCCTTTGGctc > 1:1242541/1‑68 (MQ=255) ttAGCTCCGATAACGGCAGGGTGGGCAAAATAGCCAATGGGCCCGCCGAAGACGGGGCCTGGCCCCtt > 1:3875946/1‑68 (MQ=255) ttAGCTCCGATAACGGCAGGGGGAGCGAACTAACCAATGGTCCCGCCCCAGTCGGGCCCTGGCctctc > 1:686099/1‑67 (MQ=255) gCTCCGATAACGGCAGGGTGGGGAAACTAGCCAATGGGCCAGCCGGAGGCGGGGCTTGGGCTCttttt > 1:2279777/1‑68 (MQ=255) gCTCCGATAACGGCAGGGTGGGCAAAATAGCCAAAGGGCCCGCCGAAGACGGGCCCTGGCCCCttttt > 1:3336851/1‑68 (MQ=255) gCTCCGATAACGGCAGGGTGGGCAAAAAAGCCCAAAGTCGAGCCGCAGTAGGGCCCTGGCGGCttttt > 1:564230/1‑68 (MQ=255) cTCCGATAACGGCAGGGTGAGGAAAATAGCCAATGGTCCCGCCCAGGTGCGGCCTTTGGCCCtttttt > 1:1149532/1‑68 (MQ=255) cgcggTTTAACAATCTACCCAATGGCCCAGCCAAAGTCGGGCCTTGGCCTCTTTTTTTACTTTCGCCt < 1:1800535/65‑1 (MQ=255) gTTGAGCAAACTAGCCAATGGTCCAGCCGAAGTCGGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCAc > 1:3454492/1‑68 (MQ=255) ttGAGCAAACTAGCCAATGGGCCAGCCGAAGTCGGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCCCg > 1:880341/1‑68 (MQ=255) | ATTTTGCCTTTCTGTACTCAGCCACGCTGCTATGTAATCTGGGGTTAGCTCCGATAACGGCAGGTTGAGCAAACTAGCCAATGGTCCAGCCGAAGTCGGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACG > NC_000913/296440‑296571 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |