Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	482,502	C→A	W634C (TGG→TGT)	acrB ←	multidrug efflux pump RND permease AcrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	482,502	0	C	A	100.0%	144.0 / NA	39	W634C (TGG→TGT)	acrB	multidrug efflux pump RND permease AcrB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (10/29);  total (10/29)

GAAAGCGCGTGTTGCACGCATGGTAATCGCTTCAACTTTGTTTTCTTCGCCCGGACGATCGGCCCAGTCCTTCAAGGAAACGAACGCAATACCGGTATTCTGACCACGTCCCGCAAAGC  >  NC_000913/482439‑482557                                                                |                                                        gaAAGCGCGTGTTGCACGCATGGTAATCGCTTCAACTTTGTTTTCTTCGCCCGGACGATCGGCACAGt                                                     <  1:1060366/68‑1 (MQ=255) gaAAGCGCGTGTTGCACGCATGGTAATCGCTTCAACTTTGTTTTCTTCGCCCGGACGATCGGCACAGt                                                     <  1:1798816/68‑1 (MQ=255) gaAAGCGCGTGTTGCACGCATGGTAATCGCTTCAACTTTGTTTTCTTCGCCCGGACGATCGGCACAGt                                                     <  1:3253355/68‑1 (MQ=255)  aaaGCGCGTGTTGCACGCATGGTAATCGCTTCAACTTTGTTTTCTTCGCCCGGACGATCGGCACAGTc                                                    <  1:2966517/68‑1 (MQ=255)   aaGCGCGTGTTGCACGCATGGTAATCGCTTCAACTTTGTTTTCTTCGCCCGGACGATCGGCACAGTcc                                                   <  1:1123862/68‑1 (MQ=255)   aaGCGCGTGTTGCACGCATGGTAATCGCTTCAACTTTGTTTTCTTCGCCCGGACGATCGGCACAGTcc                                                   <  1:2382180/68‑1 (MQ=255)        cgTGTTGCACGCATGGTAATCGCTTCAACTTTGTTTTCTTCGCCCGGACGATCGGCACAGTCCTTCaa                                              >  1:89556/1‑68 (MQ=255)             tGCACGCATGGTAATCGCTTCAACTTTGTTTTCTTCGCCCGGACGATCGGCACAGTCCTTCAAGGaaa                                         >  1:2555892/1‑68 (MQ=255)                aCGCATGGTAATCGCTTCAACTTTGTTTTCTTCGCCCGGACGATCGGCACAGTCCTTCAAGGAaacga                                      <  1:70082/68‑1 (MQ=255)                aCGCATGGTAATCGCTTCAACTTTGTTTTCTTCGCCCGGACGATCGGCACAGTCCTTCAAGGAaacga                                      >  1:695087/1‑68 (MQ=255)                  gCATGGTAATCGCTTCAACTTTGTTTTCTTCGCCCGGACGATCGGCACAGTCCTTCAAGGAaacgaac                                    <  1:4069431/68‑1 (MQ=255)                     tGGTAATCGCTTCAACTTTGTTTTCTTCGCCCGGACGATCGGCACAGTCCTTCAAGGAAACGAACGCa                                 <  1:1052424/68‑1 (MQ=255)                     tGGTAATCGCTTCAACTTTGTTTTCTTCGCCCGGACGATCGGCACAGTCCTTCAAGGAAACGAACGCa                                 <  1:809309/68‑1 (MQ=255)                     tGGTAATCGCTTCAACTTTGTTTTCTTCGCCCGGACGATCGGCACAGTCCTTCAAGGAAACGAACGCa                                 <  1:393429/68‑1 (MQ=255)                     tGGTAATCGCTTCAACTTTGTTTTCTTCGCCCGGACGATCGGCACAGTCCTTCAAGGAAACGAACGCa                                 <  1:3682925/68‑1 (MQ=255)                     tGGTAATCGCTTCAACTTTGTTTTCTTCGCCCGGACGATCGGCACAGTCCTTCAAGGAAACGAACGCa                                 <  1:2845703/68‑1 (MQ=255)                     tGGTAATCGCTTCAACTTTGTTTTCTTCGCCCGGACGATCGGCACAGTCCTTCAAGGAAACGAACGCa                                 <  1:1767779/68‑1 (MQ=255)                     tGGTAATCGCTTCAACTTTGTTTTCTTCGCCCGGACGATCGGCACAGTCCTTCAAGGAAACGAACGCa                                 <  1:270872/68‑1 (MQ=255)                       gTAATCGCTTCAACTTTGTTTTCTTCGCCCGGACGATCGGCACAGTCCTTCAAGGAAACGAACGCAAt                               >  1:1258044/1‑68 (MQ=255)                       gTAATCGCTTCAACTTTGTTTTCTTCGCCCGGACGATCGGCACAGTCCTTCAAGGAAACGAACGCAAt                               <  1:3512235/68‑1 (MQ=255)                          aTCTCTTCAACTTTGTTTTCTTCGCCCGGACGATCGGCACAGTCCTTCAAGGAAACGAACGCAATAcc                            <  1:1747430/68‑1 (MQ=255)                          aTCGCTTCAACTTTGTTTTCTTCGCCCGGACGATCGGCACAGTCCTTCAAGGATACGAACGCAATAcc                            <  1:3158310/68‑1 (MQ=255)                          aTCGCTTCAACTTTGTTTTCTTCGCCCGGACGATCGGCACAGTCCTTCAAGGAAACGAACGCAATAcc                            >  1:3463107/1‑68 (MQ=255)                          aTCGCTTCAACTTTGTTTTCTTCGCCCGGACGATCGGCACAGTCCTTCAAGGAAACGAACGCAATAcc                            >  1:90354/1‑68 (MQ=255)                          aTCGCTTCAACTTTGTTTTCTTCGCCCGGACGATCGGCACAGTCCTTCAAGGAAACGAACGCAATAcc                            <  1:3196384/68‑1 (MQ=255)                          aTCGCTTCAACTTTGTTTTCTTCGCCCGGACGATCGGCACAGTCCTTCAAGGAAACGAACGCAATAcc                            >  1:3108628/1‑68 (MQ=255)                          aTCGCTTCAACTTTGTTTTCTTCGCCCGGACGATCGGCACAGTCCTTCAAGGAAACGAACGCAATAcc                            <  1:1982809/68‑1 (MQ=255)                          aTCGCTTCAACTTTGTTTTCTTCGCCCGGACGATCGGCACAGTCCTTCAAGGAAACGAACGCAATAcc                            <  1:2911473/68‑1 (MQ=255)                            cGCTTCAACTTTGTTTTCTTCGCCCGGACGATCGGCACAGTCCTTCAAGGAAACGAACGCAATACCgg                          >  1:1966106/1‑68 (MQ=255)                            cGCTTCAACTTTGTTTTCTTCGCCCGGACGATCGGCACAGTCCTTCAAGGAAACGAACGCAATACCgg                          >  1:3518983/1‑68 (MQ=255)                               ttCAACTTTGTTTTCTTCGCCCGGACGATCGGCACAGTCCTTCAAGGAAACGAACGCAATACCGGTAt                       <  1:1747957/68‑1 (MQ=255)                               ttCAACTTTGTTTTCTTCGCCCGGACGATCGGCACAGTCCTTCAAGGAAACGAACGCAATACCGGTAt                       <  1:1002533/68‑1 (MQ=255)                               ttCAACTTTGTTTTCTTCGCCCGGACGATCGGCACAGTCCTTCAAGGAAACGAACGCAATACCGGTAt                       <  1:2436907/68‑1 (MQ=255)                                  aaCGTTGTTTTCTTCGCCCGGACGATCGGCACAGTCCTTCAAGGAAACGAACGCAATACCGGTATTCt                    <  1:17457/68‑1 (MQ=255)                                   aCTTTGTTTTCTTCGCCCGGACGATCGGCACAGTCCTTCAAGGAAACGAACGCAATACCGGTATTCTg                   >  1:900570/1‑68 (MQ=255)                                    cTTTGTTTTCTTCGCCCGGACGATCGGCACAGTCCTTCAAGGAAACGAACGCAATACCGGTATTCTGa                  <  1:3206594/68‑1 (MQ=255)                                                    cGGACGATCGGCACAGTCCTTCAAGGAAACGAACGCAATACCGGTATTCTGACCACGTCCCGCAaagc  <  1:3281559/68‑1 (MQ=255)                                                    cGGACGATCGGCACAGTCCTTCAAGGAAACGAACGCAATACCGGTATTCTGACCACGTCCCGCAaagc  <  1:3358730/68‑1 (MQ=255)                                                    cGGACGATCGGCACAGTCCTTCAAGGAAACGAACGCAATACCGGTATTCTGACCACGTCCCGCAaagc  <  1:1322667/68‑1 (MQ=255)                                                                |                                                        GAAAGCGCGTGTTGCACGCATGGTAATCGCTTCAACTTTGTTTTCTTCGCCCGGACGATCGGCCCAGTCCTTCAAGGAAACGAACGCAATACCGGTATTCTGACCACGTCCCGCAAAGC  >  NC_000913/482439‑482557

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑