Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,905,761	G→A	G25D (GGT→GAT)	mntP →	Mn(2(+)) exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,905,761	0	G	A	100.0%	140.4 / NA	39	G25D (GGT→GAT)	mntP	Mn(2(+)) exporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/13);  total (26/13)

CACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTC  >  NC_000913/1905696‑1905825                                                                  |                                                                 cACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATg                                                                <  1:1326594/68‑1 (MQ=255)  aCTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGc                                                               >  1:227709/1‑68 (MQ=255)  aCTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGc                                                               >  1:488220/1‑68 (MQ=255)  aCTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGc                                                               >  1:558716/1‑68 (MQ=255)     gctgctNTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCAc                                                            <  1:2611820/68‑1 (MQ=255)     gctgctGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCAc                                                            <  1:1634023/68‑1 (MQ=255)     gCTACTGTTCTTCTTGCCTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCAc                                                            <  1:856125/68‑1 (MQ=255)        aCTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCt                                                         >  1:4004040/1‑68 (MQ=255)          tGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTcc                                                       <  1:3215236/68‑1 (MQ=255)          tGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTcc                                                       <  1:3024576/68‑1 (MQ=255)          tGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTcc                                                       >  1:3335833/1‑68 (MQ=255)          tGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTcc                                                       >  1:4001249/1‑68 (MQ=255)            ttcttcTTGCGTTTGGTATGTCGAAGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCAt                                                     >  1:4069212/1‑68 (MQ=255)             tcttctTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTcctca                                                    >  1:3129590/1‑65 (MQ=255)             tcttctTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATa                                                    >  1:377620/1‑68 (MQ=255)             tcttctTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATa                                                    >  1:3432820/1‑68 (MQ=255)                  ttGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCg                                               >  1:224470/1‑68 (MQ=255)                               gTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAg                                  <  1:2351747/68‑1 (MQ=255)                               gTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAg                                  <  1:2264552/68‑1 (MQ=255)                                tCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGc                                 <  1:34031/68‑1 (MQ=255)                                 cGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGct                                >  1:1683548/1‑67 (MQ=255)                                   atggatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCAtt                              <  1:269033/68‑1 (MQ=255)                                   atggatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCAtt                              <  1:1215571/68‑1 (MQ=255)                                    tggatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTg                             >  1:2216707/1‑68 (MQ=255)                                    tggatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTg                             >  1:1970710/1‑68 (MQ=255)                                      gatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGcc                           >  1:2548397/1‑67 (MQ=255)                                      gatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCg                           >  1:3901466/1‑68 (MQ=255)                                      gatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCg                           <  1:272426/68‑1 (MQ=255)                                      gatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCg                           >  1:1008227/1‑68 (MQ=255)                                        tgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGaa                         >  1:3108610/1‑68 (MQ=255)                                            tttGCTGCATCAATCGGTAAAGATCCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCgg                     >  1:277183/1‑68 (MQ=255)                                             ttGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGc                    >  1:3997734/1‑68 (MQ=255)                                               gctgcATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCt                  >  1:1687001/1‑68 (MQ=255)                                                ctgcATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCtt                 >  1:889667/1‑68 (MQ=255)                                                 tgcATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTa                >  1:4090945/1‑68 (MQ=255)                                                  gcATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTAt               <  1:3679188/68‑1 (MQ=255)                                                     tCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTAtttt            >  1:2559413/1‑68 (MQ=255)                                                     tCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTAtttt            >  1:1186466/1‑68 (MQ=255)                                                         tCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGt        >  1:2562389/1‑68 (MQ=255)                                                         tCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGt        >  1:83197/1‑68 (MQ=255)                                                         tCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGAATTGCGAACCGGCCTTATTTTTGGt        >  1:2412635/1‑68 (MQ=255)                                                              aaaGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGt   <  1:2686834/68‑1 (MQ=255)                                                              aaaGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGt   >  1:4012493/1‑68 (MQ=255)                                                              aaaGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGt   <  1:1056735/68‑1 (MQ=255)                                                               aaGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTc  <  1:2354760/68‑1 (MQ=255)                                                                  |                                                                 CACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTC  >  NC_000913/1905696‑1905825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑