Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,223,4100TG50.0% 1.8 / 15.8 16V409G (GTG→GGG) dldD‑lactate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (1/7);  new base G (8/0);  total (9/7)
Fisher's exact test for biased strand distribution p-value = 1.40e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAA  >  NC_000913/2223346‑2223470
                                                                |                                                            
tACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCtggt                                                              <  1:2100751/65‑1 (MQ=255)
  cGAGCATCATCTGCTGTTAAAAATGGCGGGCGGGGGCGTGGGCGGAGCCAAAACGTGGCtggggg                                                            >  1:1689500/1‑65 (MQ=255)
  cGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCtggggg                                                            >  1:1955525/1‑65 (MQ=255)
   gAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGa                                                           <  1:1110566/65‑1 (MQ=255)
       atcatcTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGGGGgttgt                                                       >  1:802957/1‑63 (MQ=255)
       atcatcTGCTGTTAAAAAGGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGattat                                                       <  1:2742925/65‑1 (MQ=255)
           tcTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGGGGGTTTTTTct                                                   >  1:821941/1‑64 (MQ=255)
           tcTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGGGGGTTAttttc                                                   >  1:889058/1‑63 (MQ=255)
           tcTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGGGGGGTTTTTCa                                                   >  1:1044431/1‑65 (MQ=255)
           tcTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGGGGGGTAtttta                                                   >  1:188795/1‑63 (MQ=255)
            ctgctgTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCaa                                                  <  1:353604/65‑1 (MQ=255)
              gctgTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGGGGGTTTTTTTaaaa                                                >  1:424269/1‑64 (MQ=255)
                tgTTAAAAATGGCGGGCGATGGCGTGGGCGCAGCCAAATCGTGGTTGGGGGGTTATTTCAAGCAg                                              >  1:337878/1‑65 (MQ=255)
                tgTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGGGGGTTTTTTTCAACaa                                              >  1:2676957/1‑64 (MQ=255)
                        aTGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGGGGGTTATTTTAAAAAGGGCGAAgg                                      >  1:140300/1‑65 (MQ=255)
                            cgggcgATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGAt                                  <  1:2433261/65‑1 (MQ=255)
                                    ggCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGt                          <  1:1878028/65‑1 (MQ=255)
                                    ggCGTGGGCGAAGCCAAATCGTGGCTGGGGGAATATTTCAAACAAGCCGAAGGCGATTTCTTTGt                          >  1:1456180/1‑65 (MQ=255)
                                      cGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCt                        <  1:2373665/65‑1 (MQ=255)
                                      cGTGGGCGAAGCCAAATCGTGGCTGGGGGGTTTTTTTAAAAAGGCCGGAGGGGGTTTTTTTTTCt                        >  1:1003159/1‑65 (MQ=255)
                                      cGTGGGCGAAGCCAAATCGTGGCTGGGGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCt                        >  1:3219662/1‑65 (MQ=255)
                                           gCGAAGCCAAATCGTGGCTGGTGGGTTATTTTAAACAGGCCGAAGGCGATTTCTTTGTCTGTACg                   >  1:1873845/1‑65 (MQ=255)
                                           gCGAAGCCAAATCGTGGCTGGTGGGTTATTTCAAAAAGGCCCAAGGCGATTTCTTTGTCTGTACg                   >  1:2277603/1‑65 (MQ=255)
                                           gCGAAGCCAAATCGTGGCTGGGGGATTATTTTAAACAGGCCGAAGGCGTTTTCTTTGTCTGTACg                   >  1:2946720/1‑65 (MQ=255)
                                           gCGAAGCCAAATCGTGGCTGGGGGATTATTTCAAACAAGCCGAAGGCGATTTCTTTGTCTGTACg                   >  1:2477655/1‑65 (MQ=255)
                                              aaGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCg                <  1:1143509/65‑1 (MQ=255)
                                                 ccAAATCGTGGCTGGGGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCggag             >  1:1188853/1‑65 (MQ=255)
                                                     aTCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGaat         <  1:2184028/65‑2 (MQ=255)
                                                     aTCGTGGCTGGGGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGGGGAAg         >  1:768907/1‑65 (MQ=255)
                                                       cGTGGCTGGGGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGgc       >  1:815530/1‑65 (MQ=255)
                                                            cTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCaa  <  1:409161/65‑1 (MQ=255)
                                                                |                                                            
TACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAA  >  NC_000913/2223346‑2223470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑