Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	481,421	C→A	A995S (GCT→TCT)	acrB ←	multidrug efflux pump RND permease AcrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	481,421	0	C	A	100.0%	84.1 / NA	24	A995S (GCT→TCT)	acrB	multidrug efflux pump RND permease AcrB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (11/13);  total (11/13)

GTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGT  >  NC_000913/481360‑481472                                                              |                                                    gTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGAAcc                                                  <  1:2707634/65‑1 (MQ=255)       gTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGAACCAGTACt                                            <  1:70801/65‑1 (MQ=255)       gTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGAACCAGTACt                                            <  1:1305589/65‑1 (MQ=255)       gTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGAACCAGTACt                                            <  1:1836442/65‑1 (MQ=255)        tCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGAACCAGTACTg                                           >  1:998907/1‑65 (MQ=255)                ccGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGAACCAGTACTGATAACCAg                                   >  1:2071997/1‑65 (MQ=255)                  gCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGAACCAGTACTGATAACCAGCg                                 >  1:242672/1‑65 (MQ=255)                    ccATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGAACCAGTACTGATAACCAGCGGc                               <  1:2028710/65‑1 (MQ=255)                    ccATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGAACCAGTACTGATAACCAGCGGc                               <  1:1053507/65‑1 (MQ=255)                    ccATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGAACCAGTACTGATAACCAGCGGc                               <  1:65174/65‑1 (MQ=255)                    ccATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGAACCAGTACTGACAACCAGCGGc                               >  1:3141865/1‑65 (MQ=255)                         acacCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGAACCAGTACTGATAACCAGCGGCATAAc                          >  1:353437/1‑65 (MQ=255)                                tACCTACTGCGTTCTGCGCGCCGGAACCAGAACCAGTACTGATAACCAGCGGCATAACGCCGAgg                   <  1:1734236/65‑1 (MQ=255)                                tACCTACTGCGTTCTGCGCGCCGGAACCAGAACCAGTACTGATAACCAGCGGCATAACGCCGAgg                   <  1:669158/65‑1 (MQ=255)                                tACCTACTGCGTTCTGCGCGCCGGAACCAGAACCAGTACTGATAACCAGCGGCATAACGCCGAgg                   <  1:592359/65‑1 (MQ=255)                                 aCCTACTGCGTTCTGCGCGCCGGAACCAGAACCAGTACTGATAACCAGCGGCATAACGCCGAGGa                  >  1:203929/1‑65 (MQ=255)                                 aCCTACTGCGTTCTGCGCGCCGGAACCAGAACCAGTACTGATAACCAGCGGCATAACGCCGAGGa                  >  1:1624911/1‑65 (MQ=255)                                   cTACTGCGTTCTGCGCGCCGGAACCAGACCCAGTACTGATAACCAGCGGCATAACGCAGAGGATa                >  1:339960/1‑65 (MQ=25)                                     aCTGCGTTCTGCGCGCCGGAACCAGAACCAGTACTGATAACCAGCGGCATAACGCCGAGGATaaa              >  1:867436/1‑65 (MQ=255)                                     aCTGCGTTCTGCGCGCCGGAACCAGAACCAGTACTGATAACCAGCGGCATAACGCCGAGGATaaa              >  1:408028/1‑65 (MQ=255)                                      cTGCGTTCTGCGCGCCGGAACCAGAACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAAc             <  1:1686901/65‑1 (MQ=255)                                          gTTCTGCGCGCCGGAACCAGAACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCa         >  1:1120946/1‑65 (MQ=255)                                          gTTCTGCGCGCCGGAACCAGAACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCa         >  1:1714449/1‑65 (MQ=255)                                            tCTGCGCGCCGGAACCAGAACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGc       >  1:3329926/1‑65 (MQ=255)                                            tCTGCGCGCCGGAACCAGAACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGc       >  1:652195/1‑65 (MQ=255)                                            tCTGCGCGCCGGAACCAGAACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGc       >  1:1388463/1‑65 (MQ=255)                                              tGCGCGCCGGAACCAGAACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGa     <  1:1173916/65‑1 (MQ=255)                                                cgcgcCGGAACCAGAACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAgg   <  1:2341607/65‑1 (MQ=255)                                                 gcgcCGGAACCAGAACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGt  <  1:368371/65‑1 (MQ=255)                                                 gcgcCGGAACCAGAACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGt  <  1:2057204/65‑1 (MQ=255)                                                              |                                                    GTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGT  >  NC_000913/481360‑481472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑