Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,905,761	G→A	G25D (GGT→GAT)	mntP →	Mn(2(+)) exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,905,761	0	G	A	100.0%	108.3 / NA	30	G25D (GGT→GAT)	mntP	Mn(2(+)) exporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/13);  total (17/13)

TGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGT  >  NC_000913/1905699‑1905819                                                               |                                                           tGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATg                                                          >  1:1875277/1‑65 (MQ=255)  gCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGc                                                         <  1:996955/65‑1 (MQ=255)     gcTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCAc                                                      <  1:835354/64‑1 (MQ=255)     aCTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCAc                                                      >  1:1230177/1‑65 (MQ=255)     aCTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCAc                                                      <  1:323370/65‑1 (MQ=255)     aCTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCAc                                                      <  1:1994598/65‑1 (MQ=255)     aCTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCAc                                                      >  1:1689497/1‑65 (MQ=255)       tGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCAccc                                                    >  1:905768/1‑65 (MQ=255)       tGTTCTTCTTGCGTTTGGTATGCCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCAccc                                                    >  1:1936147/1‑65 (MQ=255)          tcttctTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTcc                                                 >  1:2512102/1‑65 (MQ=255)          tcttctTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTcc                                                 >  1:2669028/1‑65 (MQ=255)                         tATGTCGATGGATGCATTTGCTGCATGAATCGGTAAAGATGCCACCCTCCATAAACCGAAAtttt                                  >  1:382271/1‑65 (MQ=255)                           tGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGACATTTTCt                                >  1:2010179/1‑65 (MQ=255)                             tCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACACTCCATAAACCGAAATTTTCTGa                              >  1:2894208/1‑65 (MQ=255)                               gatggataCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAg                            >  1:1135162/1‑65 (MQ=255)                                atggatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGc                           <  1:3078953/65‑1 (MQ=255)                                atggatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGc                           <  1:907892/65‑1 (MQ=255)                                atggatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGc                           <  1:284488/65‑1 (MQ=255)                                 tggatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGACGCa                          >  1:3055511/1‑65 (MQ=255)                                   gatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCAtt                        >  1:1674678/1‑65 (MQ=255)                                   gatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCAtt                        >  1:1400497/1‑65 (MQ=255)                                   gatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCAtt                        >  1:1381113/1‑65 (MQ=255)                                   gatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCAtt                        <  1:41746/65‑1 (MQ=255)                                      gCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCg                     >  1:574617/1‑65 (MQ=255)                                       cATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGa                    <  1:3215577/65‑1 (MQ=255)                                             ctgcATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGc              <  1:1966741/65‑1 (MQ=255)                                              tgcATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGcc             >  1:45212/1‑65 (MQ=255)                                              tgcATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGcc             >  1:2425849/1‑65 (MQ=255)                                              tgcATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGcc             >  1:2733976/1‑65 (MQ=255)                                                  gcAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTAt         <  1:881235/64‑1 (MQ=255)                                                   cAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGGATTGCGAACCGGCCTTAtt        >  1:1643894/1‑65 (MQ=255)                                                    aaTCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTAttt       <  1:656630/65‑1 (MQ=255)                                                      tCGGTAAAGATGCCACCCTCCATAAACTGAAATTTTCTGAACCATTGCGAACCGGCCTTAttttt     >  1:544820/1‑65 (MQ=255)                                                         gTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGt  <  1:2730966/65‑1 (MQ=255)                                                               |                                                           TGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGT  >  NC_000913/1905699‑1905819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑