Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	482,533	G→A	T624I (ACC→ATC)	acrB ←	multidrug efflux pump RND permease AcrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	482,533	0	G	A	87.5%	16.7 / ‑3.4	8	T624I (ACC→ATC)	acrB	multidrug efflux pump RND permease AcrB
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (1/6);  total (2/6)
Fisher's exact test for biased strand distribution p-value = 2.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.25e-01

CAACTTTGTTTTCTTCGCCCGGACGATCGGCCCAGTCCTTCAAGGAAACGAACGCAATACCGGTATTCTGACCACGTCCCGCAAAGCCGAAGCCGTTAACGGCGAACACCGACTCAACGTTGT  >  NC_000913/482471‑482593                                                               |                                                             cAACTTTGTTTTCTTCGCCCGGACGATCGGCCCAGTCCTTCAAGGAAACGAACGCAATACCGATATTc                                                         <  1:1991722/68‑1 (MQ=255)   aCTTTGTTTTCTTCGCCCGGACGATCGGCCCAGTCCTTCAAGGAAACGAACGCAATACCGATATTCTg                                                       >  1:992085/1‑68 (MQ=255)          tttCTTCGCCCGGACGATCGGCCCAGTCCTTCAAGGAAACGAACGCAATACCGATATTCTGACCACGt                                                <  1:2569333/68‑1 (MQ=255)                   ccGGACGATCGGCCCAGTCCTTCAAGGAAACGAACGCAATACCGATATTCTGACCACGTCCCGCAaag                                       <  1:1326447/68‑1 (MQ=255)                    cGGACGATCGGCCCAGTCCTTCAAGGAAACGAACGCAATACCGATATTCTGACCACGTCCCGCAaagc                                      <  1:2574416/68‑1 (MQ=255)                    cGGACGATCGGCCCAGTCCTTCAAGGAAACGAACGCAATACCGATATTCTGACCACGTCCCGCAaagc                                      <  1:2639424/68‑1 (MQ=255)                    cGGACGATCGGCCCAGGCCTTCAAGGAAACGAACGCAATACCGATATTCTGACCACGTCCCGCAaagc                                      <  1:2144742/68‑1 (MQ=255)                                    tCCTTCAAGAAAACGAACGCAATACCGATATTCTGACCACGTCCCGCAAAGCCGAAGCCGTTAACGGc                      <  1:1780286/68‑1 (MQ=255)                                     tcTTCAAGGAAACGAACGCAATACCGGTATTCTGACCACGTCCCGCAAAGCCGAAGCCGTTAACGGCg                     >  1:1375080/2‑68 (MQ=255)                                                        aaTGCCGATATGCCGCCCCCGCCCCGCAAAGCCGAAGCCGTTAACGGCGAACAGCGACTCAACgttgt  <  1:2922102/68‑1 (MQ=21)                                                               |                                                             CAACTTTGTTTTCTTCGCCCGGACGATCGGCCCAGTCCTTCAAGGAAACGAACGCAATACCGGTATTCTGACCACGTCCCGCAAAGCCGAAGCCGTTAACGGCGAACACCGACTCAACGTTGT  >  NC_000913/482471‑482593

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑