Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	883,616	T→G	intergenic (‑228/‑57)	ybjG ← / → mdfA	undecaprenyl pyrophosphate phosphatase/multidrug efflux pump MdfA/Na(+):H(+) antiporter/K(+):H(+) antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	883,616	0	T	G	100.0%	186.2 / NA	54	intergenic (‑228/‑57)	ybjG/mdfA	undecaprenyl pyrophosphate phosphatase/multidrug efflux pump MdfA/Na(+):H(+) antiporter/K(+):H(+) antiporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (35/19);  total (35/19)

GCGTAGCTATACTCGTAATAATGTAAGAATGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCTTTCTCGTTAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCGAAGAAATTGCATGCAAA  >  NC_000913/883551‑883679                                                                  |                                                                gCGTAGCTATACTCGTAATAATGTAAGAATGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCTTgct                                                               <  1:1152030/68‑1 (MQ=255) gCGTAGCTATACTCGTAATAATGTAAGAATGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCTTgct                                                               <  1:372869/68‑1 (MQ=255) gCGTAGCTATACTCGTAATAATGTAAGAATGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCTTgct                                                               <  1:3269383/68‑1 (MQ=255) gCGTAGCTATACTCGTAATAATGTAAGAATGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCTTgct                                                               <  1:3141382/68‑1 (MQ=255) gCGTAGCTATACTCGTAATAATGTAAGAATGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCTTgct                                                               <  1:2960368/68‑1 (MQ=255) gCGTAGCTATACTCGTAATAATGTAAGAATGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCTTgct                                                               <  1:1383839/68‑1 (MQ=255) gCGTAGCTATACTCGTAATAATGTAAGAATGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCTTgct                                                               <  1:295623/68‑1 (MQ=255) gCGTAGCTATACTCGTAATAATGTAAGAATGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCTTgct                                                               <  1:2074851/68‑1 (MQ=255) gCGTAGCTATACTCGTAATAATGTAAGAATGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCTTgct                                                               <  1:1661220/68‑1 (MQ=255) gCGTAGCTATACTCGTAAGAATGTAAGAATGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCTTgct                                                               <  1:2379260/68‑1 (MQ=255) cagTAGCTATACTCGTAATAATGTAAGAATGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCTTgct                                                               <  1:304767/66‑1 (MQ=255)   gTAGCTATACTCGTAATAATGTAAGAATGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCTTGCTCg                                                             <  1:995301/68‑1 (MQ=255)        tataCTCGTAATAATGTAAGAATGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCTTGCTCGTTAGc                                                        >  1:1301063/1‑68 (MQ=255)        tataCTCGTAATAATGTAAGAATGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCTTGCTCGTTAGc                                                        >  1:146468/1‑68 (MQ=255)        tataCTCGTAATAATGTAAGAATGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCTTGCTCGTTAGc                                                        >  1:2051904/1‑68 (MQ=255)        tataCTCGTAATAATGTAAGAATGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCTTGCTCGTTAGc                                                        >  1:2513421/1‑68 (MQ=255)        tataCTCGTAATAATGCAAGAATGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCTTGCTCGTTAGc                                                        >  1:708548/1‑68 (MQ=255)        tataCTCGTAATAACGTAAGAATGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCTTGCTCGTTAGc                                                        >  1:2603774/1‑68 (MQ=255)           aCTCGTAATAATGTAAGAATGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCTTGCTCGTTAGCTgc                                                     >  1:2722985/1‑68 (MQ=255)           aCTCGTAATAATGTAAGAATGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCTTGCTCGTTAGCTgc                                                     >  1:652165/1‑68 (MQ=255)           aCTCGTAATAATGTAAGAATGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCTTGCTCGTTAGCTgc                                                     >  1:2373931/1‑68 (MQ=255)           aCTCGTAATAATGTAAGAATGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCTTGCTCGTTAGCTgc                                                     >  1:2665497/1‑68 (MQ=255)           aCTCGTAATAATGTAAGAATGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCTTGCTCGTTAGCTgc                                                     >  1:2568495/1‑68 (MQ=255)             tCGTAATCATGTAAGAATGTGCTTAACCGTGGTTTCAGCCACAAAATTCGCTTGCTCGTTAGCTgcgc                                                   >  1:2123186/1‑68 (MQ=255)             tCGTAATAATGTAAGAATGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCTTGCTCGTTAGCTgcgc                                                   >  1:943433/1‑68 (MQ=255)             tCGTAATAATGTAAGAATGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCTTGCTCGTTAGCTgcgc                                                   >  1:472112/1‑68 (MQ=255)             tCGTAATAATGTAAGAATGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCTTGCTCGTTAGCTgcgc                                                   >  1:2950785/1‑68 (MQ=255)                  ataatGTAAGAATGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCTTGGTCGTTAGCTGCGCTTtta                                              <  1:1483843/68‑1 (MQ=255)                  ataatGTAAGAATGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCTTGCTCGTTAGCTGCGCTTtta                                              >  1:2462857/1‑68 (MQ=255)                   taatGTAAGAATGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCTTGCTCGTTAGCTGCGCTTttat                                             <  1:473564/68‑1 (MQ=255)                   taatGTAAGAATGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCTTGCTCGTTAGCTGCGCTTttat                                             <  1:549432/68‑1 (MQ=255)                   taatGTAAGAATGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCTTGCTCGTTAGCTGCGCTTttat                                             <  1:2731387/68‑1 (MQ=255)                   taatGTAAGAATGTGCTTAACCGTGGTTGCAGCTACAAAATTCGCTTGCTCGTTAGCTGCGCTTttat                                             <  1:907439/68‑1 (MQ=255)                     atGTAAGAATGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCTTGCTCGTTAGCTGCGCTTttatta                                           >  1:2943691/1‑68 (MQ=255)                     atGTAAGAATGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCTTGCTCGTTAGCTGCGCTTttatta                                           >  1:944680/1‑68 (MQ=255)                     atGTAAGAATGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCTTGCTCGTTAGCTGCGCTTttatta                                           >  1:296144/1‑68 (MQ=255)                            aaTGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCTTGCTCGTTAGCTGCGCTTTTATTAAACTCTg                                    >  1:2551992/1‑68 (MQ=255)                            aaTGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCTTGCTCGTTAGCTGCGCTTTTATTAAACTCTg                                    >  1:1897800/1‑68 (MQ=255)                            aaTGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCTTGCTCGTTAGCTGCGCTTTTATTAAACTCTg                                    >  1:2991169/1‑68 (MQ=255)                            aaTGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCTTGCTCGTTAGCTGCGCTTTTATTAAACTCTg                                    >  1:3122430/1‑68 (MQ=255)                            aaTGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCTTGCTCGTTAGCTGCGCTTTTATTAAACTCNg                                    >  1:1276297/1‑68 (MQ=255)                             aggtgCTTAACCGTGGTTTCAGCTACAAAATTCGCTTGCTCGTTAGCTGCGCTTTTATTAAACTCTgc                                   <  1:2999019/66‑1 (MQ=255)                              tgtgCTTAACCGTGGTTTCAGCTACAAAATTCGCTTGCTCGTTAGCTGCGCTTTTATTAAACTCTgcg                                  >  1:18096/1‑68 (MQ=255)                                tgCTTAACCGTGGTTTCAGCTACAAAATTCGCTTGCTCGTTAGCTGCGCTTTTATTAAACTCTGcgcg                                <  1:2906212/68‑1 (MQ=255)                                 gCTTAACCGTGGTTTCAGCTACAAAATTCGCTTGCTCGTTAGCTGCGCTTTTATTAAACTCTGCGCGa                               >  1:1210045/1‑68 (MQ=255)                                 gCTTAACCGTGGTTTCAGCTACAAAATTCGCTTGCTCGTTAGCTGCGCTTTTATTAAACTCTGCGCGa                               >  1:2220252/1‑68 (MQ=255)                                  cTTAACCGTGGTTTCAGCTACAAAATTCGCTTGCTCGTTATCTGCGCTTTTATTAAACTCTGCGCGat                              >  1:2803803/1‑68 (MQ=255)                                  cTTAACCGTGGTTTCAGCTACAAAATTCGCTTGCTCGTTAGCTGCGCTTTTATTAAACTCTGCGCGat                              >  1:2687976/1‑68 (MQ=255)                                  cTTAACCGTCGTTTCAGCTACAAAATTCGCTTGCTCGTTAGCTGCGCTTTTATTAAACTCTGCGCGat                              <  1:405771/68‑1 (MQ=255)                                      aCCGTGGTTTCAGCTACAAAATTCGCTTGCTCGTTAGCTGCGCTTTTATTAAACTCTGCGCGattatt                          >  1:1721974/1‑68 (MQ=255)                                      aCCGTGGTTTCAGCTACAAAATTCGCTTGCTCGTTAGCTGCGCTTTTATTAAACTCTGCGCGattatt                          >  1:2946361/1‑68 (MQ=255)                                        cGTGGTTTCAGCTACAAAATTCGCTTGCTCGTTAGCTGCGCTTTTATTAAACTCTGCGCGattattat                        >  1:2462132/1‑68 (MQ=255)                                         gTGGTTTCAGCTACAAAATTCGCTTGCTCGTTAGCTGCGCTTTTATTAAATTCTGCGCGattattttt                       >  1:1501497/1‑68 (MQ=255)                                           ggTTTCAGCTACAAAATTCGCTTGCTCGTTAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTgg                     <  1:1313390/68‑1 (MQ=255)                                           ggTTTCAGCTACAAAATTCGCTTGCTCGTTAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTgg                     >  1:1610883/1‑68 (MQ=255)                                             tttCAGCTACAAAATTCGCTTGCTCGTTAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCg                   >  1:963500/1‑68 (MQ=255)                                               tCAGCTGCAAAATTCGCTTGCTCGTTAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCgga                 <  1:197635/68‑3 (MQ=255)                                               tCAGCTACAAAATTCGCTTGCTCGTTAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCgga                 <  1:1106350/68‑3 (MQ=255)                                               tCAGCTACAAAATTCGCTTGCTCGTTAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCgga                 <  1:858912/68‑3 (MQ=255)                                               tCAGCTACAAAATTCGCTTGCTCGTTAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCgga                 <  1:2540506/68‑3 (MQ=255)                                               tCAGCTACAAAATTCGCTTGCTCGTTAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCgga                 <  1:2925257/68‑3 (MQ=255)                                               tCAGCTACAAAATTCGCTTGCTCGTTAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCgga                 <  1:1179733/68‑3 (MQ=255)                                                 aGCTACAAAATTCGCTTGCTCGTTAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCggaga               <  1:2791902/68‑1 (MQ=255)                                                       aaaaTTCGCTTGCTCGTTAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCGGAGAAATtgc         >  1:353532/1‑68 (MQ=255)                                                            tCGCTTGCTCGTTAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCGGAGAAATtgcatgca    >  1:656138/1‑68 (MQ=255)                                                              gCTTGCTCGTTAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCGGAGAAATTGCATGCaaa  <  1:628027/68‑1 (MQ=255)                                                              gCTTGCTCGTTAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCGGAGAAATTGCATGCaaa  <  1:2304327/68‑1 (MQ=255)                                                              gCTTGCTCGTTAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCGGAGAAATTGCATGCaaa  <  1:442348/68‑1 (MQ=255)                                                              gCTTGCTCGTTAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCGGAGAAATTGCATGCaaa  <  1:1749406/68‑1 (MQ=255)                                                                  |                                                                GCGTAGCTATACTCGTAATAATGTAAGAATGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCTTTCTCGTTAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCGAAGAAATTGCATGCAAA  >  NC_000913/883551‑883679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑