Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	883,663	A→G	intergenic (‑275/‑10)	ybjG ← / → mdfA	undecaprenyl pyrophosphate phosphatase/multidrug efflux pump MdfA/Na(+):H(+) antiporter/K(+):H(+) antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	883,663	0	A	G	100.0%	159.8 / NA	47	intergenic (‑275/‑10)	ybjG/mdfA	undecaprenyl pyrophosphate phosphatase/multidrug efflux pump MdfA/Na(+):H(+) antiporter/K(+):H(+) antiporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/20);  total (27/20)

AGCTACAAAATTCGCTTTCTCGTTAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCGAAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTT  >  NC_000913/883599‑883725                                                                 |                                                               aGCTACAAAATTCGCTTGCTCGTTAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCggaga                                                             <  1:2791902/68‑1 (MQ=255)       aaaaTTCGCTTGCTCGTTAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCGGAGAAATtgc                                                       >  1:353532/1‑68 (MQ=255)            tCGCTTGCTCGTTAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCGGAGAAATtgcatgca                                                  >  1:656138/1‑68 (MQ=255)              gCTTGCTCGTTAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCGGAGAAATTGCATGCaaa                                                <  1:2304327/68‑1 (MQ=255)              gCTTGCTCGTTAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCGGAGAAATTGCATGCaaa                                                <  1:442348/68‑1 (MQ=255)              gCTTGCTCGTTAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCGGAGAAATTGCATGCaaa                                                <  1:1749406/68‑1 (MQ=255)              gCTTGCTCGTTAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCGGAGAAATTGCATGCaaa                                                <  1:628027/68‑1 (MQ=255)                  gctcGTTAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCGGAGAAATTGCATGCAaaataa                                            <  1:2693567/67‑1 (MQ=255)                  gctcGTTAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCGGAGAAATTGCATGCAaaataa                                            <  1:755902/67‑1 (MQ=255)                       ttAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCGGAGAAATTGCATGCAAAATAAATTAg                                       <  1:3373294/68‑1 (MQ=255)                        tAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCGGAGAAATTGCATGCAAAATAAAttctg                                      >  1:927704/1‑65 (MQ=255)                          gCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCGGAGAAATTGCATGCCAAATAAATTAGCtt                                    >  1:1622497/1‑68 (MQ=255)                          gCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCGGAGAAATTGCATGCAAAATAAATTAGCtt                                    >  1:563349/1‑68 (MQ=255)                          gCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCGGAGAAATTGCATGCAAAATAAATTAGCtt                                    >  1:2580847/1‑68 (MQ=255)                          gCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCGGAGAAATTGCATGCAAAATAAATTAGCtt                                    >  1:826535/1‑68 (MQ=255)                          gCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCGGAGAAATTGCATGCAAAATAAATTAGCtt                                    >  1:3130629/1‑68 (MQ=255)                          gCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCGGAGAAATTGCATGCAAAATAAATTAGCtt                                    >  1:3198016/1‑68 (MQ=255)                          gCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCGGAGAAATTGCATGCAAAATAAATTAGCtt                                    >  1:2276357/1‑68 (MQ=255)                          gCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCGGAGAAATTGCATGCAAAATAAATTAGCtt                                    >  1:2201691/1‑68 (MQ=255)                          gCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCGGAGAAATTGCATGCAAAATAAATTAGCtt                                    >  1:3349831/1‑68 (MQ=255)                          gCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCGGAGAAATTGCATGCAAAATAAATTAGCtt                                    >  1:71844/1‑68 (MQ=255)                          gCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCGGAGAAATTGCATGCAAAATAAATTAGCtt                                    >  1:717669/1‑68 (MQ=255)                          gCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCGGAGAAATTGCATGCAAAATAAATTAGCtt                                    >  1:379638/1‑68 (MQ=255)                          gCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCGGAGAAATTGCATGCAAAATAAATTAGCtt                                    >  1:661862/1‑68 (MQ=255)                          gCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCGGAGAAATTGCATGCAAAATAAATTAGCtt                                    >  1:1971166/1‑68 (MQ=255)                          gCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCGGAGAAATTGCATGCAAAATAAATTAGCtt                                    >  1:1832488/1‑68 (MQ=255)                          gCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCGGAGAAATTGCATGCAAAATAAATTAGCtt                                    >  1:1503264/1‑68 (MQ=255)                            tGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCGGAGAAATTGCATGCAAAATAAATTAGCTTcc                                  >  1:2036336/1‑68 (MQ=255)                              cgcTTTTATTAAACTCTGCGCGATTATTATTGGCGGAGAACTTGCATGCAAAATAAATTAGCTTCCgg                                >  1:1432353/1‑68 (MQ=255)                                 ttttATTAAACTCTGCGCGATTATTATTGGCGGAGAAATTGCATGCAAAATAAATTAGCTTTCGGTGc                             >  1:1226793/1‑68 (MQ=255)                                    tattaAACTCTGCGCGATTATTATTGGCGGAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAg                          <  1:1445015/68‑1 (MQ=255)                                      ttaAACTCTTCGCGATTATTATTGGCGGAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGc                        <  1:2050476/68‑1 (MQ=255)                                      ttaAACTCTGCGCGATTGTTATTGGCGGAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGc                        <  1:2489648/68‑1 (MQ=255)                                      ttaAACTCTGCGCGATTATTATTGGCGGAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGc                        <  1:3203470/68‑1 (MQ=255)                                      ttaAACTCTGCGCGATTATTATTGGCGGAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGc                        <  1:1449320/68‑1 (MQ=255)                                      ttaAACTCTGCGCGATTATTATTGGCGGAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGc                        <  1:580877/68‑1 (MQ=255)                                      ttaAACTCTGCGCGATTATTATTGGCGGAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGc                        <  1:1715185/68‑1 (MQ=255)                                          aCTCTGCGCGATTATTATTGGCGGAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTgg                    >  1:1120643/1‑68 (MQ=255)                                          aCTCTGCGCGATTATTATTGGCGGAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTgg                    >  1:3062288/1‑68 (MQ=255)                                          aCTCTGCGCGATTATTATTGGCGGAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTgg                    >  1:2339094/1‑68 (MQ=255)                                           ctctGCGCGATTATTATTGGCGGAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGa                   <  1:3354586/68‑1 (MQ=255)                                            tctGCGCGATTATTATTGGCGGAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGAc                  >  1:3019568/1‑68 (MQ=255)                                            tctGCGCGATTATTATTGGCGGAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGAc                  >  1:2015051/1‑68 (MQ=255)                                            tctGCGCGATTATTATTGGCGGAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGAc                  >  1:3222732/1‑68 (MQ=255)                                            tctGCGCGATTATTATTGGCGGAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGAc                  >  1:3174237/1‑68 (MQ=255)                                             ctGCGCGATTATTATTGGCGGAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACg                 <  1:1623199/68‑1 (MQ=255)                                             ctGCGCGATTATTATTGGCGGAAAAATTGCATGCAAAATAAATTAGCTGCCGGTGCCAGGCTTGGACg                 <  1:2166721/68‑1 (MQ=255)                                                  cgATTATTATTGGCGGAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAgg            <  1:2056180/68‑1 (MQ=255)                                                  cgATTATTATTGGCGGAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAgg            <  1:2188608/68‑1 (MQ=255)                                                  cgATTATTATTGGCGGAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAgg            <  1:879070/68‑1 (MQ=255)                                                        ttattGGCGGCGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTAc      <  1:2513665/68‑1 (MQ=255)                                                        ttattGGCGGAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTAc      <  1:1455127/68‑1 (MQ=255)                                                        ttattGGCGGAGAAAGTGCATGCAAAATCAATCAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTAc      <  1:97200/68‑1 (MQ=255)                                                         tattGGCGGAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACt     >  1:716199/1‑68 (MQ=255)                                                         tattGGCGGAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACt     >  1:144940/1‑68 (MQ=255)                                                          ataggCGGAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCGGGCTTGGACGTCAGGCGTTACtt    <  1:907561/65‑1 (MQ=255)                                                           ttGGCGGAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACttt   >  1:1611080/1‑68 (MQ=255)                                                            tGGCGGAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACtttt  <  1:132567/68‑1 (MQ=255)                                                                 |                                                               AGCTACAAAATTCGCTTTCTCGTTAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCGAAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTT  >  NC_000913/883599‑883725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑