Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,560,455	+G	pseudogene (151/758 nt)	glpR ←	DNA‑binding transcriptional repressor GlpR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,560,455	1	.	G	100.0%	49.2 / NA	16	pseudogene (151/758 nt)	glpR	DNA‑binding transcriptional repressor GlpR
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (8/8);  total (8/8)

CTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCT  >  NC_000913/3560394‑3560505                                                               |                                                   cTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGcgca                                                  >  1:1582956/1‑62 (MQ=255)                 ggCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTc                                  >  1:1809210/1‑65 (MQ=255)                  gCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTcc                                 <  1:1226583/65‑1 (MQ=255)                  gCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTcc                                 >  1:122306/1‑65 (MQ=255)                   cGTGTTAAGCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCg                                <  1:1852924/65‑1 (MQ=39)                       ttAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGTGTCAGGTTTTGCTCCGCCAg                            <  1:470704/65‑1 (MQ=255)                          aCCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTc                         >  1:2595157/1‑65 (MQ=255)                           ccGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCa                        <  1:928335/65‑1 (MQ=255)                           ccGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCa                        <  1:538685/65‑1 (MQ=255)                           ccGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCa                        >  1:486421/1‑65 (MQ=255)                                cTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAg                   >  1:181752/1‑65 (MQ=255)                                 tGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGCTTTGCTCCGCCCGCTCATTGAgg                  >  1:1850295/1‑65 (MQ=255)                                    aaGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTcg               >  1:2916063/1‑65 (MQ=255)                                    aaGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTcg               >  1:859979/1‑65 (MQ=255)                                       gCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCgcgg            <  1:54559/65‑1 (MQ=255)                                            gccgcACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAAt       >  1:1834452/1‑65 (MQ=255)                                               gccccGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGt    <  1:2111180/62‑1 (MQ=39)                                               gcACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGt    <  1:203070/65‑1 (MQ=255)                                                 aCCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCt  >  1:2540726/1‑65 (MQ=255)                                                               |                                                   CTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCT  >  NC_000913/3560394‑3560505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑