| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,374,040 | 0 | C | T | 25.0% | 34.4 / 12.3 | 24 | F273F (TTC→TTT) ‡ | ycjO | putative ABC transporter membrane subunit YcjO |
| Reads supporting (aligned to +/- strand): ref base C (7/11); new base T (0/6); total (7/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.30e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.79e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACCGCCTTTGCCTTTAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATGGCAACAAATAAA > NC_000913/1373984‑1374104 | aCCGCCTTTGCCTTTAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGTGCTCTTCATCattat > 1:697337/1‑65 (MQ=255) cgtttAGTGATCTAGCAAAATCCGGTGCGTCCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTc < 1:3067899/63‑1 (MQ=255) cgtttAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTc < 1:2414410/63‑1 (MQ=255) cctttAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTc < 1:3139075/65‑1 (MQ=255) cctttAATGATGTAGGCAAGTCGGCGGCGATCTTGGTGGTGCTCTTCATCATTATTTTCGCTGTc < 1:3067298/65‑1 (MQ=255) ctttAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGTGCTCTTCATTATTTTTTTCGCTGTCa > 1:1857254/1‑65 (MQ=255) gtttAGGGGAAGCCGCGTCGATCTCGGTGGT‑CGCCGTATCATTATTTTCGCTGTCATTCTGCTGa < 1:1465332/64‑1 (MQ=255) ggttAGGAAGAGCCGCGTAGATCTCGGTGGT‑CGCCGTATCATTATTTTCGCTGTCATTCTGCTGa < 1:2231062/63‑1 (MQ=255) aTTTAGGAAAAGCGGCGGCGGTCTCCGCGGTGCTCTTTATCATTATTTTCTCTGTCATTcttctg > 1:1683221/1‑65 (MQ=255) ggAAAAGGGGCGTAGATCTCGGTGGT‑CGCCGTATCATTATTTTCGCTGTCATTCTGCTGACCAgg < 1:2789594/65‑1 (MQ=255) gAAAAGCGGCGGCGATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAgg < 1:1359307/65‑1 (MQ=255) gAAAAGCGGCGGCGATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAgg < 1:1667022/65‑1 (MQ=255) gAAAAGCGGCGGCGATCTCGGTGGTACTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAgg < 1:1474628/65‑1 (MQ=255) aaGGGGCGGCGATCTCGGTGGT‑CGCCGTATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAAc < 1:1789855/65‑1 (MQ=255) tcgATCTCGGTGGT‑CGCCGTATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAAc < 1:1838305/64‑1 (MQ=255) gcgATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGaa < 1:2359243/65‑1 (MQ=255) cgATCTCGGTGGT‑CGCCGTATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAAcc < 1:2132290/65‑1 (MQ=255) cgATCTCGGTGGT‑CGCCGTATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAAcc < 1:1916273/65‑1 (MQ=255) agATCTCGGTGGT‑CGCCGTATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAAcc < 1:1887546/64‑1 (MQ=255) gATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAAcc < 1:2302806/65‑1 (MQ=255) ctcGGTGGTGCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCa < 1:3137535/65‑1 (MQ=255) tcGGTGGTGCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCaa > 1:339400/1‑65 (MQ=255) gtggtgCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATgg < 1:559870/65‑1 (MQ=255) ggtgCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATGGca > 1:1983477/1‑65 (MQ=255) ggtgCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATGGca < 1:1822405/65‑1 (MQ=255) tgCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATGGcaac > 1:309583/1‑65 (MQ=255) gCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATGGcaaca > 1:673180/1‑65 (MQ=255) tctTCATCATTATTTTCGCTGTCATTCTGCTGACTAGGAAACGGGTGAACCTCAATGGCAACaaa > 1:2142399/1‑65 (MQ=255) tctTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATGGCAACaaa > 1:687469/1‑65 (MQ=255) tctTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGAGAACCTCAATGGCAACaaa > 1:1573045/1‑65 (MQ=255) catcatTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATGGCAACAAATaaa < 1:2318679/65‑1 (MQ=255) catcatTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATGGCAACAAATaaa < 1:2549287/65‑1 (MQ=255) | ACCGCCTTTGCCTTTAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATGGCAACAAATAAA > NC_000913/1373984‑1374104 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |