Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,390,754	G→T	A252A (GCC→GCA)	rsgA ←	ribosome small subunit‑dependent GTPase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,390,754	0	G	T	100.0%	66.1 / NA	19	A252A (GCC→GCA)	rsgA	ribosome small subunit‑dependent GTPase A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (13/6);  total (13/6)

AACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGA  >  NC_000913/4390692‑4390809                                                               |                                                        aaCTCACGCACTCCTTGGGAGTCAATCACATCCCCGCCGTGCGGGAAGTGATACAGCCGAgctgc                                                       <  1:3051255/65‑1 (MQ=255)  aCTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAgctgcg                                                      >  1:2505992/1‑65 (MQ=255)   cTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGctgcgg                                                     >  1:863070/1‑65 (MQ=255)    tcactcacTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGt                                                    <  1:1682036/65‑1 (MQ=255)    tCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGt                                                    <  1:1558355/65‑1 (MQ=255)       cGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCgt                                                 <  1:2699685/65‑1 (MQ=255)          aCTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCgtggt                                              >  1:2698306/1‑65 (MQ=255)                ggggAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTg                                        >  1:460571/1‑65 (MQ=255)                    aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGAccc                                    >  1:2103628/1‑64 (MQ=255)                    aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                    >  1:1355045/1‑65 (MQ=255)                    aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                    >  1:1796891/1‑65 (MQ=255)                    aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                    >  1:2068884/1‑65 (MQ=255)                    aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                    >  1:2846154/1‑65 (MQ=255)                      tCAATCACATCCCCGCCGTGCGGGAAGTGATCCAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAg                                  <  1:110593/65‑1 (MQ=255)                                    gccgTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGCGGTGGCag                    >  1:2826739/1‑65 (MQ=255)                                    gccgTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCag                    >  1:1521367/1‑65 (MQ=255)                                    gccgTGCGGGAAGTGATACAGCCGAGCTGCGGTCGCGGTGTGCTGACCGAGCCCCGAGATGTCag                    >  1:908739/1‑65 (MQ=255)                                     ccgTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCaga                   <  1:2326659/65‑1 (MQ=255)                                       gTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAgaga                 <  1:102013/65‑1 (MQ=255)                                         gCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGata               >  1:264717/1‑65 (MQ=255)                                                 tGATACAGCCGAGCTGCGGCCGTGGTGTGCTGAACGAGCCCCGAGTTGGCAGAGATATCGTTGGt       >  1:1531543/1‑65 (MQ=255)                                                     aCAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAgg   >  1:2227779/1‑65 (MQ=255)                                                      cAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGa  >  1:840663/1‑65 (MQ=255)                                                               |                                                        AACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGA  >  NC_000913/4390692‑4390809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑