Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,905,761	G→A	G25D (GGT→GAT)	mntP →	Mn(2(+)) exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,905,761	0	G	A	100.0%	99.4 / NA	28	G25D (GGT→GAT)	mntP	Mn(2(+)) exporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/9);  total (19/9)

TGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATT  >  NC_000913/1905699‑1905813                                                               |                                                     tGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATg                                                    >  1:1008342/1‑65 (MQ=255) tGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATg                                                    <  1:1653048/65‑1 (MQ=255)  gCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGc                                                   >  1:112051/1‑65 (MQ=255)     aCTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCAc                                                <  1:476684/65‑1 (MQ=255)     aCTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCAc                                                >  1:3059835/1‑65 (MQ=255)     aCTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCAc                                                >  1:1833888/1‑65 (MQ=255)      cTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCAcc                                               <  1:648006/65‑1 (MQ=255)       tGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCAccc                                              >  1:2495216/1‑65 (MQ=255)          tcttctTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTcc                                           >  1:1686207/1‑65 (MQ=255)          tcttctTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTcc                                           >  1:1893764/1‑65 (MQ=255)          tcttctTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTcc                                           >  1:695159/1‑65 (MQ=255)          tcttctTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTcc                                           >  1:401524/1‑65 (MQ=255)          tcttctTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTcc                                           <  1:2497170/65‑1 (MQ=255)                         tATGTCGATGGATGCATTTGCTGCATCCATCGGTAAAGATGCCCCCCTCCATAAACCGAAAtttt                            <  1:2854285/65‑1 (MQ=255)                         tATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAAtttt                            >  1:2732658/1‑65 (MQ=255)                           tGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCt                          <  1:2134715/65‑1 (MQ=255)                                 tggatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCa                    >  1:2317991/1‑65 (MQ=255)                                      gCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCg               >  1:319885/1‑65 (MQ=255)                                      gCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCg               >  1:2223011/1‑65 (MQ=255)                                      gCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCg               >  1:2028792/1‑65 (MQ=255)                                       cATTTGCTGCATCAATCGGTAAAGATGCCCCCCTCCATAAACCGAAATTTTCTGAAGCATTGCGa              <  1:626141/65‑1 (MQ=255)                                       cATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGa              >  1:356910/1‑65 (MQ=255)                                       cATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGa              >  1:788881/1‑65 (MQ=255)                                          ttGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAAcc           >  1:2831439/1‑65 (MQ=255)                                          ttGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAAcc           >  1:778845/1‑65 (MQ=255)                                            gctgcATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCgg         >  1:2473039/1‑65 (MQ=255)                                             ctgcATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGc        <  1:1068704/65‑1 (MQ=255)                                              tgcATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGcc       >  1:1389/1‑65 (MQ=255)                                              tgcATCAATCGGTAAAGATGCCACCCTCCATAAACAGAAATTTTCTGAAGCATGGCGAACCggtc       >  1:3088289/1‑63 (MQ=255)                                                  tCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTAt   <  1:2837957/65‑1 (MQ=255)                                                   cAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTAtt  <  1:1999490/65‑1 (MQ=255)                                                               |                                                     TGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATT  >  NC_000913/1905699‑1905813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑