| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,087,406 | 0 | A | C | 52.6% | 4.8 / 28.3 | 19 | W285G (TGG→GGG) | yeeY | putative DNA‑binding transcriptional regulator YeeY |
| Reads supporting (aligned to +/- strand): ref base A (9/0); new base C (0/10); total (9/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.08e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.61e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTTCCGTCATCAGATTTTGCATAAACACCGGCGATCGCACTATTTGCTAAAATTT‑CATCCCGCCACCAGCCACTGGCAAGTCCTGCGGTCTGTTCGTTCCAGCCAATCCAGACGGGTTCAAAGAGA > NC_000913/2087342‑2087467 | tttCCGTCATCAGATTTTGCATAAACACCGGCGATCGCACTATTTGCTAAAATTT‑CATCCCGcca > 1:1882336/1‑65 (MQ=255) ttCCGTCATCAGATTTTGCATAAACACCGGCGATCGCACTATTTGCTAAAATTT‑CATCCCGccac > 1:3325464/1‑65 (MQ=255) ttcatcaGATTTTGCATAAACCCGGGCGATCGCACTTTTTGCAAAAATTT‑CACCCCGCCCCCAGc < 1:838195/64‑1 (MQ=255) tcaGATTTTGCAAAACCACCGGCGATCGCACTTTTGGCAAAATTTT‑CACCCCGCCCCCAGCCACt < 1:1997584/65‑1 (MQ=255) aGATTTTGCATAACCCCCGGCGACCGCATTTTTTGCTAAAATTT‑CACCCCGCCCCCAGCCACTgg < 1:4310974/65‑1 (MQ=255) ttccATAACCNCCGGCGATCGCGTTATTTGCTAAATTTT‑CACCCCCCCCCCAGCCACTGGCAAGt < 1:1318933/62‑1 (MQ=255) ttccATAACCCCCGGAGATCGCTTTATTTGCAAAATTTT‑CACCCCGCCCCCAGCCACTGGCAAGt < 1:330872/62‑1 (MQ=255) ttccATAAACCCCGGCGATCGCTTTATTTGCAAAAATTT‑CACCCCGCCCCCAGCCACTGGCAAGt < 1:1237976/62‑1 (MQ=255) ttGCATAAACCCCGGCGATCGCATTTTTTGCAAAAATTT‑CACCCCGCCCCCAGCCACTGGCAAGt < 1:4403376/65‑1 (MQ=255) ttGCAAAACCCCCGGCGACCGCTTTATTTGCAAAAATTT‑CACCCCGCCCCCAGCCACTGGCAAGt < 1:2441847/65‑1 (MQ=255) ttGCAAAAACCCCGGCGATCGCATTATTGGCTAAATTTC‑CACCCCGCCCCCAGCCACTGGCAAGt < 1:3201582/65‑1 (MQ=255) tggCATAAACCCCGGCGATCGCATTTTTTGCTAAATTTT‑CACCCCGCCCCCAGCCACTGGCAAGt < 1:1587693/63‑1 (MQ=255) tggCATAAACACCGGCGACCGCCTTTTTTGCTAAATTTT‑CACCCCGCCCCCAGCCACTGGCAAGt < 1:2995026/63‑1 (MQ=255) aTAAACACCGGCGATCGCACTATTTGCTAAAATTT‑CATCCCGCCACCAGCCACTGGCAAGTCCTg > 1:1827541/1‑65 (MQ=255) aTAAACACCGGCGATCGCACTATTTGCTAAAATTT‑CATCCCGCCACCAGCCACTGGCAAGTCCTg > 1:799823/1‑65 (MQ=255) aaCCCCGGCGATCGCATTATTTCCAAAAATTT‑CACCCCGCCCCCAGCCACTGGCAAGTCCTGCgg < 1:2246767/65‑1 (MQ=255) cGGCGATCGCATTTTTGGCTAAATTTT‑CCCCCCGCCCCCAGCCACTGGCAAGTCCTGCGGTCTgt < 1:3138813/65‑1 (MQ=255) cGGCCACCGCATTTTTTGCAAAAATTT‑CGTCCCGCCCCCAGCCACTGGCAAGTCCTGCGGTCTgt < 1:1905312/65‑1 (MQ=255) cGATCGCACTATTTGCTAAAATTT‑CATCCCGCCACCAGCCACTGGCAAGTCCTGCGGTCTgttcg > 1:2561024/1‑65 (MQ=255) gctCGCATTTTTTGCTAAAATTT‑CACCCCGCCCCCAGCCACTGGCAAGTCCTGCGGTCTgttcgt < 1:3631490/63‑1 (MQ=255) aCTATTTGCTAAAATTT‑CATCCCGCCACCAGCCACTGGCAAGTCCTGCGGTCTGTTCGTTCCAGc > 1:1792871/1‑65 (MQ=255) aCTATTTGCTAAAATTT‑CATCCCGCCACCAGCCACTGGCAAGTCCTGCGGTCTGTTCGTTCCAGc > 1:2934697/1‑65 (MQ=255) tATTTGCTAAAATTT‑CATCCCGCCACCAGCCACTGGCAAGTCCTGCGGTCTGTTCGTTCCAGCCa > 1:760358/1‑65 (MQ=255) aTTTGCTAAAATTT‑CATCCCGCCACCAGCCACTGGCAAGTCCTGCGGTCTGTTCGTTCCAGCCaa > 1:1565038/1‑65 (MQ=255) tttGCAAAAATTT‑CCCCCCCCCCCCAGCCACTGGCAAGTCCTGCGGTCTGTTCGTTCCAGCCAAt < 1:28995/65‑1 (MQ=255) tttGCAAAAATTC‑CCCCCCCCCCCCAGCCACTGGCAAGTCCTGCGGTCTGTTCGTTCCAGCCAAt < 1:1277233/65‑1 (MQ=255) tttcTAAAATTTCCCCCCCCCCCCCAGCCACTGGCAAGTCCTGCGGTCTGTTCGTTCCAGCCAAt < 1:1439185/62‑1 (MQ=255) cTAAAATTT‑CATCCCGCCACCAGCCACTGGCAAGTCCTGCGGTCTGTTCGTTCCAGCCAATCCAg > 1:1093101/1‑65 (MQ=255) aaaatTTT‑CCCCCCCCCCCCAGCCACTGGCAAGTCCTGCGGTCTGTTCGTTCCAGCCAATCCAGa < 1:1629967/64‑1 (MQ=255) accccACCCCCAGCCACTGGCGAGTCCTGCGGTCTGTTCGTTCCAGCCAATCCAGACGGGTTCaa < 1:1142999/63‑1 (MQ=255) cccccccccCAGCCACTGGCAAGTCCTGCGGTCTGTTCGTTCCAGCCAATCCAGACGGGTTCaaa < 1:671086/64‑1 (MQ=255) gCCACCAGCCACTGGCAAGTCCTGCGGTCTGTTCGTTCCAGCCAATCCAGACGGGTTCAAagaga > 1:2122590/1‑65 (MQ=255) | TTTCCGTCATCAGATTTTGCATAAACACCGGCGATCGCACTATTTGCTAAAATTT‑CATCCCGCCACCAGCCACTGGCAAGTCCTGCGGTCTGTTCGTTCCAGCCAATCCAGACGGGTTCAAAGAGA > NC_000913/2087342‑2087467 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |