Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,390,754	G→T	A252A (GCC→GCA)	rsgA ←	ribosome small subunit‑dependent GTPase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,390,754	0	G	T	100.0%	103.3 / NA	30	A252A (GCC→GCA)	rsgA	ribosome small subunit‑dependent GTPase A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (23/7);  total (23/7)

CTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCT  >  NC_000913/4390694‑4390812                                                             |                                                           cTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGctgcgg                                                        >  1:4191423/1‑65 (MQ=255)  tCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGt                                                       <  1:3723471/65‑1 (MQ=255)     cGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCgg                                                    <  1:653014/65‑2 (MQ=255)          tCCTGGGGGGTCAATCACATCACAGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGgtgt                                               <  1:3427238/65‑1 (MQ=255)          tCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGgtgt                                               <  1:3134212/65‑1 (MQ=255)                ggAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGAc                                         >  1:578887/1‑65 (MQ=255)                ggAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGAc                                         >  1:313549/1‑65 (MQ=255)                  aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                       >  1:4386462/1‑65 (MQ=255)                  aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                       >  1:3837855/1‑65 (MQ=255)                  aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                       >  1:3378515/1‑65 (MQ=255)                  aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                       >  1:2408040/1‑65 (MQ=255)                  aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                       >  1:2898182/1‑65 (MQ=255)                  aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                       >  1:2955037/1‑65 (MQ=255)                  aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCCGCCCg                                       >  1:3372481/1‑65 (MQ=255)                  aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGCGCTGACCg                                       >  1:1321094/1‑65 (MQ=255)                  aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCCGCGGCCGTGGTGCGCTGATCg                                       >  1:2081550/1‑65 (MQ=255)                    tCAATCACATCCCCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAg                                     <  1:845091/65‑1 (MQ=255)                        tcacatcacCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGcccc                                 >  1:2691761/1‑65 (MQ=255)                          acatcacCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGa                               >  1:2932762/1‑65 (MQ=255)                          acatcacCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGa                               >  1:1340910/1‑65 (MQ=255)                            atcccCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGt                             <  1:2028740/65‑1 (MQ=255)                             tcacCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGtt                            >  1:3194600/1‑65 (MQ=255)                                ccgccgTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTc                         >  1:3947556/1‑65 (MQ=255)                                  gccgTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCag                       >  1:361432/1‑65 (MQ=255)                                  gccgTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCag                       >  1:3645512/1‑65 (MQ=255)                                  gccgTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCag                       >  1:3539034/1‑65 (MQ=255)                                   ccgTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCaga                      <  1:2977002/65‑1 (MQ=255)                                         gggAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATc                >  1:1241960/1‑65 (MQ=255)                                         gggAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATc                <  1:797407/65‑1 (MQ=255)                                            gaGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGtt             <  1:3090459/64‑1 (MQ=255)                                             aGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTg            >  1:1559769/1‑65 (MQ=255)                                             aGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGCTg            >  1:3257972/1‑65 (MQ=255)                                               tGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGt          <  1:284378/65‑1 (MQ=255)                                                   aCAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAgg      >  1:1354010/1‑65 (MQ=255)                                                     aGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAt    >  1:2067600/1‑65 (MQ=255)                                                       ccGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAtct  >  1:1988910/1‑65 (MQ=255)                                                             |                                                           CTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCT  >  NC_000913/4390694‑4390812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑