Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	485,894	G→A	R45H (CGC→CAC)	acrR →	DNA‑binding transcriptional repressor AcrR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	485,894	0	G	A	100.0%	247.5 / NA	68	R45H (CGC→CAC)	acrR	DNA‑binding transcriptional repressor AcrR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (33/35);  total (33/35)

CTCACAGCAGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCTGGGAACT  >  NC_000913/485832‑485954                                                               |                                                             ctcACAGCAGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTAcgcacg                                                            <  1:3591367/65‑1 (MQ=255) ctcACAGCAGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTAcgcacg                                                            <  1:3797972/65‑1 (MQ=255)  tcACAGCAGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACgg                                                           >  1:2440122/1‑65 (MQ=255)   cacaGCAGGGGGTATCATCCTCCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACGGt                                                          <  1:1173758/65‑1 (MQ=255)   cacaGCAGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACGGt                                                          <  1:2235709/65‑1 (MQ=255)   cacaGCAGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACGGt                                                          <  1:1131087/65‑1 (MQ=255)   cacaGCAGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACGGt                                                          <  1:2304266/65‑1 (MQ=255)   cacaGCAGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACGGt                                                          <  1:2336540/65‑1 (MQ=255)   cacaGCAGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACGGt                                                          <  1:241310/65‑1 (MQ=255)   cacaGCAGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACGGt                                                          <  1:2756791/65‑1 (MQ=255)   cacaGCAGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACGGt                                                          <  1:3320573/65‑1 (MQ=255)   cacaGCAGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACGGt                                                          <  1:3594264/65‑1 (MQ=255)   cacaGCAGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACGGt                                                          <  1:3871408/65‑1 (MQ=255)   cacaGCAGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACGGt                                                          <  1:4268276/65‑1 (MQ=255)   cacaGCAGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACGGt                                                          <  1:432409/65‑1 (MQ=255)   cacaGCAGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACGGt                                                          <  1:4416143/65‑1 (MQ=255)   cacaGCAGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACGGt                                                          <  1:496610/65‑1 (MQ=255)   cacaGCAGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACGGt                                                          <  1:680606/65‑1 (MQ=255)   cacaGCAGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACGGt                                                          <  1:755890/65‑1 (MQ=255)   cacaGCAGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACGGt                                                          <  1:846161/65‑1 (MQ=255)   cacaGCAGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACGGt                                                          <  1:1054277/65‑1 (MQ=255)   cacaGCAGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACGGt                                                          <  1:1661752/65‑1 (MQ=255)   cacaGCAGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACGGt                                                          <  1:1512064/65‑1 (MQ=255)   cacaGCAGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACGGt                                                          <  1:1863101/65‑1 (MQ=255)   cacaGCAGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACGGt                                                          <  1:1401305/65‑1 (MQ=255)   cacaGCAGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACGGt                                                          <  1:1782062/65‑1 (MQ=255)   agcagcggGGGGTATCTTCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACGGt                                                          <  1:1472613/63‑1 (MQ=255)       gcagGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACGGTGCaa                                                      >  1:587272/1‑65 (MQ=255)        gcaggggTATCATCCACCTCGGTAGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACGGTGCAAt                                                     <  1:3422262/62‑1 (MQ=255)        cagGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACGGTGCAAt                                                     <  1:1827205/65‑1 (MQ=255)        cagGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACGGTGCAAt                                                     <  1:169199/65‑1 (MQ=255)        cagGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACGGTGCAAt                                                     <  1:1548739/65‑1 (MQ=255)        cagGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACGGTGCAAt                                                     <  1:3934363/65‑1 (MQ=255)         agGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACGGTGCAATc                                                    >  1:289349/1‑65 (MQ=255)          gggggTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACGGTGCAATCt                                                   <  1:3663580/65‑1 (MQ=255)          gggggTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACGGTGCAATCt                                                   <  1:539324/65‑1 (MQ=255)          gggggTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACGGTGCAATCt                                                   <  1:3391007/65‑1 (MQ=255)            gggTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACGGTGCAATCTAc                                                 >  1:3641260/1‑65 (MQ=255)            gggTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACGGTGCAATCTAc                                                 >  1:416752/1‑65 (MQ=255)            gggTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACGGTGCAATCTAc                                                 >  1:3555250/1‑65 (MQ=255)            gggTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACGGTGCAATCTAc                                                 >  1:3000924/1‑65 (MQ=255)            gggTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACGGTGCAATCTAc                                                 >  1:547623/1‑65 (MQ=255)              gTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACGGTGTAATCTACTg                                               >  1:3751571/1‑65 (MQ=255)                atcatcCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACGGTGCAATCTACTGGc                                             >  1:1439546/1‑65 (MQ=255)                atcatcCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACGGTGCAATCTACTGGc                                             >  1:1492376/1‑65 (MQ=255)                 tcatcCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACGGTGCAATCTACTGGCa                                            >  1:1315288/1‑65 (MQ=255)                 tcatcCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACGGTGCAATCTACTGGCa                                            >  1:717868/1‑65 (MQ=255)                 tcatcCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACGGTGCAATCTACTGGCa                                            >  1:3857922/1‑65 (MQ=255)                 tcatcCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACGGGGCAATCTACTGGcc                                            >  1:1290805/1‑64 (MQ=255)                   atcCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACGGTGCAATCTACTGGCAtt                                          >  1:1601796/1‑65 (MQ=255)                   atcCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACGGTGCAATCTACTGGCAtt                                          >  1:4130242/1‑65 (MQ=255)                   atcCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACGGTGCAATCTACTGGCAtt                                          >  1:4000572/1‑65 (MQ=255)                   atcCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACGGTGCAATCTACTGGCAtc                                          >  1:1737367/1‑64 (MQ=255)                   atcCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACGGCGCAATCTACTGGCAtt                                          >  1:3762146/1‑65 (MQ=255)                    tcCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACGGTGCAATCTACTGGCAttt                                         <  1:220223/65‑1 (MQ=255)                     ccACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACGGTGCAATCTACTGGCAtttt                                        >  1:1936260/1‑65 (MQ=255)                     ccACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACGGAGCAATCTACTGGCAtttt                                        >  1:2571734/1‑65 (MQ=255)                       aCCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACGGCGCAAGCTACTGGCATTTTaa                                      >  1:4300624/1‑65 (MQ=255)                        ccTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACGGTGCAATCTACTGGCATTTTaaa                                     >  1:2895896/1‑65 (MQ=255)                         cTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGGACGGAGCAATCTACTGGCATTTTAAAg                                    >  1:4276205/1‑65 (MQ=255)                         cTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACGGTGCAATCTACTGGCATTTTAAAg                                    >  1:1952141/1‑65 (MQ=255)                         cTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACGGTGCAATCTACTGGCATTTTAAAg                                    >  1:2154083/1‑65 (MQ=255)                          tCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACGGTGCAATCTACTGGCATTTTAAAGa                                   >  1:463871/1‑65 (MQ=255)                          tCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACGGTGCAATCTACTGGCATTTTAAAGa                                   >  1:1343100/1‑65 (MQ=255)                              tGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCACGGTGCAATCTACTGGCATTTTAAAGACAAg                               >  1:408971/1‑65 (MQ=255)                                   gagaTTGCAAAAGCAGCTGGCGTTACGCACGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGa                          <  1:1797333/65‑1 (MQ=255)                                      aTTGCAAAAGCAGCTGGCGTTACGCACGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGAttt                       >  1:770505/1‑65 (MQ=255)                                      aTTGCAAAAGCAGCTGGCGTTACGCACGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGAttt                       >  1:4368245/1‑65 (MQ=255)                                              agcagcTGGCGTTACGCACGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTg               >  1:452893/1‑65 (MQ=255)                                                cagcTGGCGTTACGCACGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTgag             >  1:2821840/1‑65 (MQ=255)                                                 agcTGGCGTTACGCACGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTgaga            <  1:2023562/65‑1 (MQ=255)                                                 agcTGGCGTTACGCACGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTgaga            <  1:1880578/65‑1 (MQ=255)                                                     ggCGTTACGCACGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCTg        >  1:424327/1‑65 (MQ=255)                                                       cGTTACGCACGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCTggg      >  1:3884989/1‑65 (MQ=255)                                                           aCGCACGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCTGGGAACt  <  1:251905/65‑1 (MQ=255)                                                               |                                                             CTCACAGCAGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCTGGGAACT  >  NC_000913/485832‑485954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑