Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,905,761	G→A	G25D (GGT→GAT)	mntP →	Mn(2(+)) exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,905,761	0	G	A	100.0%	132.0 / NA	36	G25D (GGT→GAT)	mntP	Mn(2(+)) exporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/16);  total (20/16)

GCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTT  >  NC_000913/1905700‑1905816                                                              |                                                        gCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGc                                                      <  1:925430/65‑1 (MQ=255)    aCTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCAc                                                   >  1:3725745/1‑65 (MQ=255)         tcttctTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTcc                                              >  1:2137905/1‑65 (MQ=255)         tcttctTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTcc                                              >  1:3399062/1‑65 (MQ=255)         tcttctTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTcc                                              <  1:204828/65‑1 (MQ=255)         tcttctTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTcc                                              <  1:1929121/65‑1 (MQ=255)         tcttctTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTcc                                              >  1:1269697/1‑65 (MQ=255)         tcttctTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGGAAAGATGCCACCCTcc                                              <  1:4082863/65‑1 (MQ=255)           ttcttGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCAt                                            >  1:1765340/1‑65 (MQ=255)            tcttGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATa                                           >  1:3640043/1‑65 (MQ=255)                gCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAAcc                                       <  1:3795559/65‑1 (MQ=255)                       gTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAAttt                                <  1:883767/65‑1 (MQ=255)                        tATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAAtttt                               >  1:1769882/1‑65 (MQ=255)                        tATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAAtttt                               <  1:3486578/65‑1 (MQ=255)                        tATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTACATAAACCGAAAtttt                               >  1:410546/1‑65 (MQ=255)                            tCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGa                           >  1:1089757/1‑65 (MQ=255)                             cGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGaa                          <  1:33977/65‑1 (MQ=255)                               atggatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGc                        <  1:2365960/65‑1 (MQ=255)                               atggatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGc                        <  1:3075680/65‑1 (MQ=255)                                  gatgCATTTGCTGCATTAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCAtt                     >  1:1108589/1‑65 (MQ=255)                                  gatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCAtt                     >  1:966548/1‑65 (MQ=255)                                  gatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCAtt                     >  1:195990/1‑65 (MQ=255)                                  gatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCAtt                     >  1:1806714/1‑65 (MQ=255)                                  gatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCAtt                     >  1:1619066/1‑65 (MQ=255)                                      cATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGa                 <  1:1742085/65‑1 (MQ=255)                                        tttGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAAc               >  1:111151/1‑65 (MQ=255)                                           gctgcATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCgg            >  1:4153468/1‑65 (MQ=255)                                           gctgcATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCgg            >  1:991382/1‑65 (MQ=255)                                           gctgcATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCgg            >  1:2482657/1‑65 (MQ=255)                                            ctgcATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGc           <  1:3346522/65‑1 (MQ=255)                                            ctgcATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGc           <  1:2185940/65‑1 (MQ=255)                                             tgcATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGcc          >  1:2466996/1‑65 (MQ=255)                                                aTCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTa       <  1:3588778/65‑1 (MQ=255)                                                 tCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTAt      <  1:395459/65‑1 (MQ=255)                                                 tCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTAt      >  1:624019/1‑65 (MQ=255)                                                   aaTCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCNTTAttt    <  1:2573188/65‑1 (MQ=255)                                                   aaTCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTAttt    <  1:1256153/65‑1 (MQ=255)                                                     tCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTAttttt  >  1:4358700/1‑65 (MQ=255)                                                     tCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTAttttt  >  1:1159611/1‑65 (MQ=255)                                                              |                                                        GCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTT  >  NC_000913/1905700‑1905816

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑