Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,390,754	G→T	A252A (GCC→GCA)	rsgA ←	ribosome small subunit‑dependent GTPase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,390,754	0	G	T	100.0%	135.6 / NA	36	A252A (GCC→GCA)	rsgA	ribosome small subunit‑dependent GTPase A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (25/11);  total (25/11)

TCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTC  >  NC_000913/4390695‑4390813                                                            |                                                            tCACGCGCTCGTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGt                                                        <  1:4246509/65‑1 (MQ=255)    cccACCCCTGGGGAGTCAATCACACCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCgt                                                     <  1:4600226/63‑1 (MQ=255)        cTCCTGGGGAGGCAATCACATCGCCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCgtggtg                                                 <  1:3000394/65‑1 (MQ=255)         tCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGgtgt                                                <  1:950946/65‑1 (MQ=255)         tCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGgtgt                                                <  1:519830/65‑1 (MQ=255)         tCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGgtgt                                                <  1:2684575/65‑1 (MQ=255)             ggggAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTg                                            >  1:2178742/1‑65 (MQ=255)              gggAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGa                                           <  1:575954/65‑1 (MQ=255)               ggAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGAc                                          >  1:1502152/1‑65 (MQ=255)               ggAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGAc                                          >  1:4448896/1‑65 (MQ=255)               ggAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGAc                                          >  1:3641966/1‑65 (MQ=255)               ggAGTCAATCACATCACCGCCGTGCGGGAAGTCATACAGCCGAGCTGCGGTCGTGGTGTGCTGAc                                          >  1:4221563/1‑65 (MQ=255)                 aGTCAATCACATCACCGCCGTGCGGGCAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                        >  1:4085436/1‑65 (MQ=255)                 aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                        >  1:4075734/1‑65 (MQ=255)                 aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                        >  1:3433013/1‑65 (MQ=255)                 aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                        >  1:3429054/1‑65 (MQ=255)                 aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                        >  1:2939942/1‑65 (MQ=255)                 aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGTGTGCTGACCg                                        >  1:1294632/1‑65 (MQ=255)                  gTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGa                                       >  1:3285074/1‑65 (MQ=255)                   tCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAg                                      <  1:4045406/65‑1 (MQ=255)                   tCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAg                                      >  1:3866120/1‑65 (MQ=255)                       tcacatcacCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGcccc                                  >  1:334837/1‑65 (MQ=255)                       tcacatcacCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGcccc                                  >  1:2480719/1‑65 (MQ=255)                         acatcacCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGa                                >  1:1262565/1‑65 (MQ=255)                                 gccgTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCag                        >  1:2885245/1‑65 (MQ=255)                                 gccgTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCag                        >  1:1057735/1‑65 (MQ=255)                                 gccgTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCag                        >  1:3676001/1‑65 (MQ=255)                                 gccgTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCag                        >  1:2799570/1‑65 (MQ=255)                                 gccgTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCag                        >  1:4704067/1‑65 (MQ=255)                                 gccgTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCag                        >  1:4052413/1‑65 (MQ=255)                                   cgTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCagag                      <  1:1040005/65‑1 (MQ=255)                                    gTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAgaga                     <  1:4580761/65‑1 (MQ=255)                                        gggAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATc                 >  1:2893421/1‑65 (MQ=255)                                            aGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTg             >  1:3958305/1‑65 (MQ=255)                                            aGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTg             >  1:284090/1‑65 (MQ=255)                                             gTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTgg            <  1:1310672/65‑1 (MQ=255)                                                  acggCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAgg       <  1:1987308/62‑1 (MQ=255)                                                  acggCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAgg       <  1:577016/62‑1 (MQ=255)                                                    aGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAt     >  1:1685827/1‑65 (MQ=255)                                                    aGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAt     >  1:4774037/1‑65 (MQ=255)                                                     gCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAtc    >  1:2000533/1‑65 (MQ=255)                                                     gCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAtc    >  1:1444123/1‑65 (MQ=255)                                                     gCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGCCAGGAtc    >  1:4106603/1‑65 (MQ=255)                                                      ccGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAtct   <  1:2661346/65‑1 (MQ=255)                                                       cGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAtctc  >  1:2323504/1‑65 (MQ=255)                                                            |                                                            TCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTC  >  NC_000913/4390695‑4390813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑