Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,619,399	C→T	R94C (CGC→TGC)	marR →	DNA‑binding transcriptional repressor MarR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,619,399	0	C	T	100.0%	91.5 / NA	26	R94C (CGC→TGC)	marR	DNA‑binding transcriptional repressor MarR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/6);  total (20/6)

ATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCA  >  NC_000913/1619339‑1619457                                                             |                                                           aTGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTgcgg                                                        >  1:687995/1‑65 (MQ=255)  tGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTgcggc                                                       <  1:4355686/65‑1 (MQ=255)   gCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGAGAAGTgcggcg                                                      >  1:3616659/1‑65 (MQ=255)   gCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTgcggcg                                                      >  1:3576980/1‑65 (MQ=255)   gCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTgcggcg                                                      >  1:2915987/1‑65 (MQ=255)   gCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTgcggcg                                                      >  1:2692169/1‑65 (MQ=255)   gCTGGATCGCCTGGTCTGTAAAGGCTGGGGGGAAAGGGTGCCGAACCCGAATGACAAGTgcggcg                                                      >  1:1694423/1‑65 (MQ=255)        aTCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTg                                                 >  1:1476773/1‑65 (MQ=255)        aTCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTg                                                 <  1:3373173/65‑1 (MQ=255)        aTCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTg                                                 >  1:1787371/1‑65 (MQ=255)         tCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTgg                                                >  1:635380/1‑65 (MQ=255)                 tCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACtt                                        >  1:1124255/1‑65 (MQ=255)                 tCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACtt                                        >  1:3419135/1‑65 (MQ=255)                   tGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGGACTGGTAAAAATTac                                      >  1:4371871/1‑65 (MQ=255)                   ggTAAAGTCTGGATGGAAAGGTTGCCGAACCCGAATGGCAAGTGCGGCGTACTGGTAAAACTTac                                      <  1:4265942/64‑1 (MQ=255)                         ggCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACcgc                                >  1:3263631/1‑64 (MQ=255)                           cTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACcggcg                              >  1:2617798/1‑65 (MQ=255)                           cTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACcggcg                              >  1:1669005/1‑65 (MQ=255)                           cTGGGTGGAAAGGTAGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACcggcg                              >  1:3673281/1‑65 (MQ=255)                             gggTGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCggcggc                            <  1:2336672/65‑1 (MQ=255)                                tGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGgcggcgcg                         >  1:3021943/1‑65 (MQ=255)                                tGGAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGgcggcgcg                         >  1:4748042/1‑65 (MQ=255)                                 ggAAAGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGgcggcgcgg                        >  1:1401848/1‑65 (MQ=255)                                    aaGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCaa                     >  1:2423348/1‑65 (MQ=255)                                     aGGTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAat                    >  1:722275/1‑65 (MQ=255)                                      ggTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAata                   >  1:346520/1‑65 (MQ=255)                                      ggTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAata                   >  1:2720428/1‑65 (MQ=255)                                      ggTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAata                   >  1:1922807/1‑65 (MQ=255)                                      ggTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAata                   >  1:1787290/1‑65 (MQ=255)                                      ggTTGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAata                   >  1:764452/1‑65 (MQ=255)                                        ttGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATAtg                 <  1:1579799/65‑1 (MQ=255)                                         tGCCGAACCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATAtgt                <  1:3562674/65‑1 (MQ=255)                                               aCCCGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGGGGTAATATGAGAACaa          >  1:1767939/1‑65 (MQ=255)                                                  cGAATGACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGc       <  1:75387/65‑1 (MQ=255)                                                       gACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGccacca  >  1:1885621/1‑65 (MQ=255)                                                       gACAAGTGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCa  >  1:3329286/1‑65 (MQ=255)                                                             |                                                           ATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCA  >  NC_000913/1619339‑1619457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑