Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,905,761	G→A	G25D (GGT→GAT)	mntP →	Mn(2(+)) exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,905,761	0	G	A	100.0%	121.4 / NA	33	G25D (GGT→GAT)	mntP	Mn(2(+)) exporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/13);  total (20/13)

TGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCC  >  NC_000913/1905699‑1905822                                                               |                                                              tGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATg                                                             >  1:1548725/1‑65 (MQ=255)  gCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGc                                                            <  1:4790597/65‑1 (MQ=255)    tACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCa                                                          >  1:659121/1‑65 (MQ=255)     aCTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCAc                                                         <  1:1660669/65‑1 (MQ=255)     aCTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCAc                                                         <  1:116449/65‑1 (MQ=255)     aCTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCAc                                                         >  1:1975074/1‑65 (MQ=255)       tGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGGCAcgc                                                       >  1:2954012/1‑63 (MQ=255)       tGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCAccc                                                       >  1:3897321/1‑65 (MQ=255)       tGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCAccc                                                       >  1:1231596/1‑65 (MQ=255)       tGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCAccc                                                       >  1:2205078/1‑65 (MQ=255)          tcttctTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTcc                                                    <  1:204732/65‑1 (MQ=255)            ttcttGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCAt                                                  >  1:3650872/1‑65 (MQ=255)                  cGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCg                                            >  1:1759924/1‑65 (MQ=255)                  cGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCg                                            >  1:7512/1‑65 (MQ=255)                        gTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAAttt                                      <  1:382123/65‑1 (MQ=255)                         tATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAAtttt                                     >  1:1174739/1‑65 (MQ=255)                            gTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTg                                  >  1:2768574/1‑65 (MQ=255)                             tCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGa                                 >  1:1936612/1‑65 (MQ=255)                                atggatgCATTTTGTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGc                              <  1:2733630/65‑1 (MQ=255)                                  ggatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCAt                            <  1:173213/65‑1 (MQ=255)                                  ggatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCAt                            <  1:3559129/65‑1 (MQ=255)                                  ggatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCAt                            <  1:43791/65‑1 (MQ=255)                                   gatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGACATTTTCTGAAGCAtt                           >  1:1538820/1‑65 (MQ=255)                                   gatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCAtt                           <  1:4320270/65‑1 (MQ=255)                                   gatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCAtt                           >  1:4662643/1‑65 (MQ=255)                                          ttGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAAcc                    >  1:1505405/1‑65 (MQ=255)                                          ttGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAAcc                    >  1:1302005/1‑65 (MQ=255)                                             ctgcATCAATCGGTAAAGATGCCGCCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGc                 <  1:3573260/65‑1 (MQ=255)                                             ctgcATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGc                 <  1:1935053/65‑1 (MQ=255)                                              tgcATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGcc                >  1:565450/1‑65 (MQ=255)                                              tgcATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGcc                >  1:280792/1‑65 (MQ=255)                                              tgcATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGcc                >  1:4530494/1‑65 (MQ=255)                                              tgcATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGcc                >  1:3292272/1‑65 (MQ=255)                                               gcATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCt               <  1:2008427/65‑1 (MQ=255)                                                      tCGGTAAACATGCCACCCTCCCTAAACCGAAATTTTCTGCAGCATTGCGACACGGCCTTAttttt        >  1:3616548/1‑65 (MQ=255)                                                           aaaGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGc   >  1:764469/1‑65 (MQ=255)                                                            aaGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGcc  >  1:3800757/1‑65 (MQ=255)                                                               |                                                              TGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCC  >  NC_000913/1905699‑1905822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑