Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,939,260	G→A	Q312* (CAG→TAG)	lpxM ←	myristoyl‑acyl carrier protein‑dependent acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,939,260	0	G	A	100.0%	237.9 / NA	63	Q312* (CAG→TAG)	lpxM	myristoyl‑acyl carrier protein‑dependent acyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/46);  total (17/46)

GGCCTCTCCTCGCGAGAGGCTTTTTTATTTGATGGGATAAAGATCTTTGCGCTTATACGGCTGGATTTCGCCCGGTTTGCGAGTTTTCAGCAATTTTAGTATCCAGGTGTATTGTTCTGGTCG  >  NC_000913/1939198‑1939320                                                               |                                                             ggCCTCTCCTCGCGAGAGGCTTTTTTATTTGATGGGATAAAGATCTTTGCGCTTATACGGCTAGa                                                            <  1:2180344/65‑1 (MQ=255) ggCCTCGCCTCGCGAGAGGCTTTTTTATTTGATGGGATAAAGATCTTTGCGCTTATACGGCTAGa                                                            <  1:1320388/65‑1 (MQ=255)  gCCTCTCCTCGCGAGAGGCTTTTTTATTTGATGGGATAAAGATCTTTGCGCTTATACGGCTAGAt                                                           >  1:787120/1‑65 (MQ=255)  gCCTCTCCTCGCGAGAGGCTTTTTTATTTGATGGGATAAAGATCTTTGCGCTTATACGGCTAGAt                                                           >  1:3004819/1‑65 (MQ=255)  gCCTCTCCTCGCGAGAGGCTTTTTTATTTGATGGGATAAAGATCTTTGCGCTTATACGGCTAGAt                                                           >  1:1540755/1‑65 (MQ=255)  gCCTCTCCTCGCGAGAGGCTTTTTTATTTGATGGGATAAAGATCTTTGCGCTTATACGGCTAGAt                                                           >  1:3178803/1‑65 (MQ=255)  gCCTCTCCCCGCGAGAGGCTTTTTTATTTGATGGGATAAAGATCTTTGCGCTTATACGGCTAGAt                                                           >  1:3181067/1‑65 (MQ=255)         ctcGCGAGAGGCTTTTTTATTTGCTGGGATAAAGATCTTTGCGCTTATACGGCTAGATTTCGccc                                                    >  1:1782484/1‑65 (MQ=255)            gcgAGAGGCTTTTTTATTTGATGGGATAAAGATCTTTGCGCTTATACGGCTAGATTTCGCCCGGt                                                 <  1:244780/65‑1 (MQ=255)            gcgAGAGGCTTTTTTATTTGATGGGATAAAGATCTTTGCGCTTATACGGCTAGATTTCGCCCGGt                                                 <  1:2484396/65‑1 (MQ=255)            gcgAGAGGCTTTTTTATTTGATGGGATAAAGATCTTTGCGCTTATACGGCTAGATTTCGCCCGGt                                                 <  1:2567372/65‑1 (MQ=255)            gcgAGAGGCTTTTTTATTTGATGGGATAAAGATCTTTGCGCTTATACGGCTAGATTTCGCCCGGt                                                 <  1:3718486/65‑1 (MQ=255)            gcgAGAGGCTTTTTTATTTGATGGGATAAAGATCTTTGCGCTTATACGGCTAGATTTCGCCCGGt                                                 <  1:3863781/65‑1 (MQ=255)            gcgAGAGGCTTTTTTATTTGATGGGATAAAGATCTTTGCGCTTATACGGCTAGATTTCGCCCGGt                                                 <  1:4212328/65‑1 (MQ=255)            gcgAGAGGCTTTTTTATTTGATGGGATAAAGATCTTTGCGCTTATACGGCTAGATTTCGCCCGGt                                                 <  1:4347272/65‑1 (MQ=255)            gcgAGAGGCTTTTTTATTTGATGGGATAAAGATCTTTGCGCTTATACGGCTAGATTTCGCCCGGt                                                 <  1:4390735/65‑1 (MQ=255)             cgAGAGGCTTTTTTATTTGATGGGATAAAGATCTTTGCGCTTATACGGCTAGATTTCGCCCGGtt                                                >  1:4098498/1‑65 (MQ=255)             cgAGAGGCTTTTTTATTTGATGGGATAAAGATCTTTGCGCTTATACGGCTAGAATTCGCCCGGtt                                                >  1:463639/1‑65 (MQ=255)                  ggCTTTTTTATTTGATGGGATAAAGATCTTTGCGCTTATACGGCTAGATTTCGCCCGGTTTGCGa                                           >  1:2195045/1‑65 (MQ=255)                    cTTTTTTATTTGATGGGATAAAGATCTTTGCGCTTATACGGCTAGATTTCGCCCGGTTTGCGAGt                                         <  1:4148371/65‑1 (MQ=255)                    cTTTTTTATTTGATGGGATAAAGATCTTTGCGCTTATACGGCTAGATTTCGCCCGGTTTGCGAGt                                         >  1:998503/1‑65 (MQ=255)                        tttATTTGATGGGATAAAGATCTTTGCGCTTATACGGCTAGAATTCGCCCCGTTTGCGCGTTTTc                                     >  1:2006002/1‑65 (MQ=255)                              tGATGGGATAAAGATCTTTGCGCTTATACGGCTAGATTTCGCCCGGTTTGCGAGTTTTCAGCAAt                               <  1:2598190/65‑1 (MQ=255)                              tGATGGGATAAAGATCTTTGCGCTTATACGGCTAGATTTCGCCCGGTTTGCGAGTTTTCAGCAAt                               <  1:2642186/65‑1 (MQ=255)                               gATGGGATAAAGATCTTTGCGCTTATACGGCTAGATTTCGCCCGGTTTGCGAGTTTTCAGCAAtt                              <  1:1524831/65‑1 (MQ=255)                                    gATAAAGATCTTTGCGCTTATACGGCTAGATTTCGCCCGGTTTGCGAGTTTTCAGCAATTTTAGt                         <  1:2511283/65‑1 (MQ=255)                                    gATAAAGATCTTTGCGCTTATACGGCTAGATTTCGCCCGGTTTGCGAGTTTTCAGCAATTTTAGt                         <  1:3060342/65‑1 (MQ=255)                                    gATAAAGATCTTTGCGCTTATACGGCTAGATTTCGCCCGGTTTGCGAGTTTTCAGCAATTTTAGt                         <  1:2977355/65‑1 (MQ=255)                                      tAAAGATCTTTGCGCTTATACGGCTAGATTTCGCCCGGTTTGCGAGTTTTCAGCAATTTTAGTAt                       <  1:2303939/65‑1 (MQ=255)                                      tAAAGATCTTTGCGCTTATACGGCTAGATTTCGCCCGGTTTGCGAGTTTTCAGCAATTTTAGTAt                       <  1:4576007/65‑1 (MQ=255)                                         aGATCTTTGCGCTTATACGGCTAGATTTCGCCCGGTTTGCGAGTTTTCAGCAATTTTAGTATCCa                    <  1:1503532/65‑1 (MQ=255)                                          gATCTTTGCGCTTATACGGCTAGATTTCGCCCGGTTTGCGAGTTTTCAGCAATTTTAGTATCCAg                   >  1:51027/1‑65 (MQ=255)                                          gATCTTTGCGCTTATACGGCTAGATTTCGCCCGGTTTCCGAGTTTTCAGCAATTTTAGTATTCAg                   >  1:4381291/1‑65 (MQ=255)                                           aTCTTTGCGCTTATACGGCTAGATTTCGCCCGGTTTGCGGGGTTTCAGCAATTTTAGTATCCAgg                  >  1:1830792/1‑65 (MQ=255)                                           aTCTTTGCGCTTATACGGCTAGATTTCGCCCGGTTTGCGAGTTTTCAGCAATTTTAGTATCCAgg                  <  1:1527299/65‑1 (MQ=255)                                           aTCTTTGCGCTTATACGGCTAGATTTCGCCCGGTTTGCGAGTTTTCAGCAATTTTAGTATCCAgg                  >  1:1685399/1‑65 (MQ=255)                                           aTCTTTGCGCTTATACGGCTAGATTTCGCCCGGTTTGCGAGTTTTCAGCAATTTTAGTATCCAgg                  <  1:3488499/65‑1 (MQ=255)                                           aTCTTTGCGCTTATACGGCTAGATTTCGCCCGGTTTGCGAGTTTTCAGCAATTTTAGTATCCAgg                  <  1:3396321/65‑1 (MQ=255)                                           aTCTTTGCGCTTATACGGCTAGATTTCGCCCGGTTTGCGAGTTTTCAGCAATTTTAGTATCCAgg                  <  1:3244050/65‑1 (MQ=255)                                           aTCTTTGCGCTTATACGGCTAGATTTCGCCCGGTTTGCGAGTTTTCAGCAATTTTAGTATCCAgg                  >  1:923600/1‑65 (MQ=255)                                           aTCTTTGCGCTTATACGGCTAGATTTCGCCCGGTTTGCGAGTTTTCAGCAATTTTAGTATCCAgg                  <  1:2212540/65‑1 (MQ=255)                                            tCTTTGCGCTTATACGGCTAGATTTCGCCCGGTTTGCGAGTTTTCAGCAATTTTAGTATCCAGgt                 >  1:1285434/1‑65 (MQ=255)                                            tCTTTGCGCTTATACGGCTAGATTTCGCCCGGTTTGCGAGTTTTCAGCAATTTTAGTATCCAGgt                 <  1:3600763/65‑1 (MQ=255)                                            tCTTTGCGCTTATACGGCTAGATTTCGCCCGGTTTGCGAGTTTTCAGCAATTTTAGTATCCAGgt                 <  1:3624020/65‑1 (MQ=255)                                            tCTTTGCGCTTATACGGCTAGATTTCGCCCGGTTTGCGAGTTTTCAGCAATTTTAGTATCCAGgt                 <  1:1650974/65‑1 (MQ=255)                                              tttGCGTTTATACGGCTAGATTTCGCCCGGTTTGCGAGTTTTCAGCAATTTTAGTATCCAGgtgt               <  1:2996801/65‑1 (MQ=255)                                              tttGCGCTTATACGGCTAGATTTCGCCCGGTTTGCGAGTTTTCAGCAATTTTAGTATCCAGgtgt               <  1:213892/65‑1 (MQ=255)                                              tttGCGCTTATACGGCTAGATTTCGCCCGGTTTGCGAGTTTTCAGCAATTTTAGTATCCAGgtgt               <  1:4555515/65‑1 (MQ=255)                                              tttGCGCTTATACGGCTAGATTTCGCCCGGTTTGCGAGTTTTCAGCAATTTTAGTATCCAGgtgt               <  1:2658774/65‑1 (MQ=255)                                              tttGCGCTTATACGGCTAGATTTCGCCCGGTTTGCGAGTTTTCAGCAATTTTAGTATCCAGgtgt               <  1:1997637/65‑1 (MQ=255)                                              tttGCGCTTATACGGCTAGATTTCGCCCGGTTTGCGAGTTTTCAGCAATTTTAGTATCCAGgtgt               <  1:807734/65‑1 (MQ=255)                                              tttGCGCTTATACGGCTAGATTTCGCCCGGTTTGCGAGTTTTCAGCAATTTTAGTATCCAGgtgt               <  1:1674575/65‑1 (MQ=255)                                              tttGCGCTTATACGGCTAGATTTCGCCCGGTTTGCGAGTTTTCAGCAATTTTAGTATCCAGgtgt               <  1:3382802/65‑1 (MQ=255)                                              tttGCGCTTATACGGCTAGATTTCGCCCGGTTTGCGAGTTTTCAGCAATTTTAGTATCCAGgtgt               <  1:1711858/65‑1 (MQ=255)                                              tttGCGCTTATACGGCTAGATTTCGCCCGGTTTGCGAGTTTTCAGCAATTTTAGTATCCAGgtgt               <  1:995542/65‑1 (MQ=255)                                              tttGCGCTTATACGGCTAGATTTCGCCCGGTTTGCGAGTTTTCAGCAATTTTAGTATCCAGgtgt               <  1:2696109/65‑1 (MQ=255)                                              tttGCGCTTATACGGCTAGATTTCGCCCGGTTTGCGAGTTTTCAGCAATTTTAGTATCCAGgtgt               <  1:301018/65‑1 (MQ=255)                                              tttGCGCTTATACGGCTAGATTTCGCACGGTTTGCGAGTTTTCAGCAATTTTAGTATCCAGgtgt               <  1:1063906/65‑1 (MQ=255)                                                tGCGCTTATACGGCTAGATTTCGCCCGGTTTGCGAGTTTTCAGCAATTTTAGTATCCAGGTGTAt             <  1:804069/65‑1 (MQ=255)                                                 gcgcTTATACGGCTAGATTTCGCCCGGTTTGCGAGTTTTCAGCAATTTTAGTATCCAGGTGTAtt            >  1:4730133/1‑65 (MQ=255)                                                 gcgcTTATACGGCTAGATTTCGCCCGGTTTGCGAGTTTTCAGCAATTTTAGTATCCAGGTGTAtt            <  1:3075896/65‑1 (MQ=255)                                                   gcTTATACGGCTAGATTTCGCCCGGTTTGCGAGTTTTCAGCAATTTTAGTATCCAGGTGTATTGt          <  1:1606182/65‑1 (MQ=255)                                                   gcTTATACGGCTAGATTTCGCCCGGTTTGCGAGTTTTCAGCAATTTTAGTATCCAGGTGTATTGt          <  1:2387395/65‑1 (MQ=255)                                                        taCGGCTAGATTTCGCCCGGTTTGCGAGTTTTCAGCAATTTTAGTATCCAGGTGTATTGTTCTgg     <  1:1478507/65‑1 (MQ=255)                                                        taCGGCTAGATTTCGCCCGGTTTGCGAGTTTTCAGCAATTTTAGTATCCAGGTGTATTGTTCTgg     <  1:859272/65‑1 (MQ=255)                                                         aCGGCTAGATTTCGCCCGGTTTGCGAGTTTTCAGCAATTTTAGTATCCAGGTGTATTGTTCTGGt    >  1:4694130/1‑65 (MQ=255)                                                           ggCTAGATTTCGCCCGGTTTGCGAGTTTTCAGCAATTTTAGTATCCAGGGGTATTGTTCTGGTcg  >  1:3236021/1‑65 (MQ=255)                                                               |                                                             GGCCTCTCCTCGCGAGAGGCTTTTTTATTTGATGGGATAAAGATCTTTGCGCTTATACGGCTGGATTTCGCCCGGTTTGCGAGTTTTCAGCAATTTTAGTATCCAGGTGTATTGTTCTGGTCG  >  NC_000913/1939198‑1939320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑