Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,395,063	Δ1 bp	coding (1196/3801 nt)	yhdP ←	AsmA2 domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,395,063	0	T	.	100.0%	138.4 / NA	29	coding (1196/3801 nt)	yhdP	AsmA2 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (23/6);  total (23/6)

AAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATCGCTCCACGATGCCTGAAAACGGGTCTTGTCTGCCGCCTGAAGCGGGATATCC  >  NC_000913/3395004‑3395116                                                            |                                                      aaaGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGA‑CGCTcc                                                 >  1:2753444/1‑65 (MQ=255)   aGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGA‑CGCTCCaa                                               >  1:4139453/1‑64 (MQ=255)   aGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGA‑CGCTCCAc                                               >  1:4280400/1‑65 (MQ=255)   aGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGA‑CGCTCCAc                                               >  1:1295888/1‑65 (MQ=255)   aGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGA‑CGCTCCAc                                               >  1:3093291/1‑65 (MQ=255)   aGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGA‑CGCTCCAc                                               >  1:133004/1‑65 (MQ=255)   aGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGA‑CGCTCCAc                                               >  1:2557765/1‑65 (MQ=255)   aGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGA‑CGCTCCAc                                               >  1:2344798/1‑65 (MQ=255)   aGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGA‑CGCTCCAc                                               >  1:1972322/1‑65 (MQ=255)   aGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCAAGA‑CGCTCCAc                                               >  1:4093777/1‑65 (MQ=255)           ggAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGA‑CGCTCCACGATGCCTg                                       >  1:2819857/1‑65 (MQ=255)              gaAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGA‑CGCTCCACGATGCCTGaaa                                    <  1:192265/65‑1 (MQ=255)                aGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGA‑CGCTCCACGATGCCTGAAAAc                                  >  1:840760/1‑65 (MQ=255)                aGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGA‑CGCTCCACGATGCCTGAAAAc                                  >  1:132344/1‑65 (MQ=255)                aGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGA‑CGCTCCACGATGCCTGAAAAc                                  >  1:1388267/1‑65 (MQ=255)                  tgtTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGA‑CGCTCCACGATGCCTGAAAACgg                                >  1:1370090/1‑65 (MQ=255)                  tgtTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGA‑CGCTCCACGATGCCTGAAAACgg                                >  1:229492/1‑65 (MQ=255)                   gtTCCGCACTCGGTAATAATTTCCATTGCTTCCAGGCCAGA‑CGCTCCACGATGCCTGAAAACggg                               <  1:1056129/65‑1 (MQ=255)                   gtTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGA‑CGCTCCACGATGCCTGAAAACggg                               >  1:2906256/1‑65 (MQ=255)                   gtTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGA‑CGCTCCACGATGCCTGAAAACggg                               <  1:4136548/65‑1 (MQ=255)                   gtTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGA‑CGCTCCACGATGCCTGAAAACggg                               <  1:1211512/65‑1 (MQ=255)                     tCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGA‑CGCTCCACGATGCCTGAAAACGGGTc                             >  1:1841349/1‑65 (MQ=255)                     tCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGA‑CGCTCCACGATGCCTGAAAACGGGTc                             >  1:1716192/1‑65 (MQ=255)                     tCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGA‑CGCTCCACGATGCCTGAAAACGGGTc                             >  1:2298256/1‑65 (MQ=255)                     tCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGA‑CGCTCCACGATGCCTGAAAACGGGTc                             >  1:485673/1‑65 (MQ=255)                      ccGCCCCCGGTAATAATTTCCATTGCTTCCAGGCCGGA‑CGCTCCACGATGCCTGAAAACGGGTCt                            <  1:2749637/65‑1 (MQ=255)                        gCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGA‑CGCTCCACGATGCCTGAAAACGGGTCTTg                          >  1:3997976/1‑65 (MQ=255)                        gCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGA‑CGCTCCACGATGCCTGAAAACGGGTCTTg                          >  1:1278014/1‑65 (MQ=255)                                      tttCCATTGCTTCCAGGCCAGA‑CGCTCCACGATGCCTGAAAACGGGTCTTGTCTGCCGCCTGAAg            >  1:1248699/1‑65 (MQ=255)                                         cccttGCTTCCAGGCCCGA‑CGCTCCACGATGCCTGAAAACGGGTCTTGTCTGCCGCCTGAAGCgg         <  1:2227114/62‑1 (MQ=255)                                         ccATTGCTTCCAGGCCAGA‑CGCTCCACGATGCCTGAAAGCGGGTCTTGTCTGCCGCCTGAAGCgg         <  1:2126141/65‑1 (MQ=255)                                         ccATTGCTTCCAGGCCAGA‑CGCTCCACGATGCCTGAAAACGGGTCTTGTCTGCCGCCTGAAGCgg         <  1:675540/65‑1 (MQ=255)                                         ccATTGCTTCCAGGCCAGA‑CGCTCCACGATGCCTGAAAACGGGTCTTGTCTGCCGCCTGAAGCgg         >  1:667099/1‑65 (MQ=255)                                         ccATTGCTTCCAGGCCAGA‑CGCTCCACGATGCCTGAAAACGGGTCTTGTCTGCCGCCTGAAGCgg         <  1:3508509/65‑1 (MQ=255)                                               cTTCCAGGCCAGA‑CGCTCCACGATGCCTGAAAACGGGTCTTGTCTGCCGCCTGAAGCGGGATATc   <  1:2424005/65‑1 (MQ=255)                                               cTTCCAGCCCAGA‑CGCTCCACGATGCCTGAAATCGGGTCTTGTCTGCCGCCTGAAGCGGGATATc   <  1:1849927/65‑1 (MQ=255)                                                ttCCAGGCCAGA‑CGCTCCACGATGCCTGAAAACGGGTCTTGTCTGCCGCCTGAAGCGGGATATcc  >  1:815271/1‑65 (MQ=255)                                                            |                                                      AAAGCGTCCCGGAGAAGTGTTCCGCACCCGGTAATAATTTCCATTGCTTCCAGGCCAGATCGCTCCACGATGCCTGAAAACGGGTCTTGTCTGCCGCCTGAAGCGGGATATCC  >  NC_000913/3395004‑3395116

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑