| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,182,442 | G→C | V400L (GTT→CTT) | rpoB → | RNA polymerase subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,182,442 | 0 | G | C | 98.1% | 173.0 / ‑2.7 | 53 | V400L (GTT→CTT) | rpoB | RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base C (32/20); total (32/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.96e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.54e-01 | |||||||||||
AAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCT > NC_000913/4182380‑4182503 | aaGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGCtt > 1:3056331/1‑65 (MQ=255) agcagcTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGCTTg > 1:3525461/1‑65 (MQ=255) agcagcTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGCTTg > 1:4195065/1‑65 (MQ=255) agcagcTGAAAGCCTGTTCGAGAACCTGTTCTCATCCGAAGACCGTTATGACTTGTCTGCGCTTg > 1:1324581/1‑65 (MQ=255) cagcTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGggtggt > 1:1991603/1‑65 (MQ=255) cagcTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGCTTGGt < 1:2912251/65‑1 (MQ=255) cagcTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGCTTGGt > 1:1585858/1‑65 (MQ=255) cagcTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGCTTGGt < 1:4335929/65‑1 (MQ=255) cagcTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGCTTGGt < 1:1709577/65‑1 (MQ=255) cagcTGAAAGCCTGTTCGAGAACCTGTTCTTCGCCGAAGACCGTTATGACTTGTCTGCGCTTGGt < 1:1638668/65‑1 (MQ=255) cTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGCTTGGTCGt < 1:425952/65‑1 (MQ=255) aGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGCTTGGTCGTATGaa < 1:217310/65‑1 (MQ=255) gCCTGTTCGAGAACTTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGCTTGGTCGCATGacg > 1:2487364/1‑63 (MQ=255) gCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGCTTTGTCGTATGact > 1:3653347/1‑63 (MQ=255) gCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGCTTTGTCGTATGAAg > 1:2087195/1‑65 (MQ=255) gCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGCTTGGTCGTATgcct > 1:974107/1‑62 (MQ=255) gCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGCTTGGTCGTATGacg > 1:4140282/1‑63 (MQ=255) gCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGCTTGGTCGTATGCAg > 1:3447189/1‑65 (MQ=255) gCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGCTTGGTCGTATGCAg > 1:4338482/1‑65 (MQ=255) gCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGCTTGGTCGTATGCAg > 1:4345337/1‑65 (MQ=255) gCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGCTTGGTCGTATGAAg > 1:1084836/1‑65 (MQ=255) gCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGCTTGGTCGTATGAAg > 1:3945027/1‑65 (MQ=255) gCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGCTTGGTCGTATGAAg > 1:2145652/1‑65 (MQ=255) gCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGCTTGGTCGTATGAAg > 1:3865610/1‑65 (MQ=255) gCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGCTTGGTCGTATGAAg > 1:2298444/1‑65 (MQ=255) gCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGCTTGGTCGTATGAAg > 1:3540052/1‑65 (MQ=255) gCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGCTTGGTCGTATGAAg > 1:4222762/1‑65 (MQ=255) gCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGCTTGGTCGTATGAAg > 1:4231282/1‑65 (MQ=255) gCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGAATTGTCTGCGCTTGGTCGTATgccg > 1:966770/1‑62 (MQ=255) ccTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGCTTGGTCGTATGAAGt > 1:204462/1‑65 (MQ=255) agaACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGt < 1:3850247/65‑1 (MQ=255) agaACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGCTTGGTCGTATGAAGTTCAACCGt < 1:3483566/65‑1 (MQ=255) agaACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGCTTGGTCGTATGAAGTTCAACCGt < 1:2548438/65‑1 (MQ=255) agaACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGCTTGGTCGTATGAAGTTCAACCGt < 1:3408838/65‑1 (MQ=255) agaACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGCTTGGTCGTATGAAGTTCAACCGt < 1:1564238/65‑1 (MQ=255) agaACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGCTTGGTCGTATGAAGTTCAACCGt > 1:2478561/1‑65 (MQ=255) agaACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGCTTGGTCGTATGAAGTTCAACCGt < 1:513895/65‑1 (MQ=255) aaCCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGCTTGGTCGTATGACGTTCTACCGTtc > 1:878120/1‑65 (MQ=255) aaCCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGCTTGGTCGTATGAAGTTCAACCGTtc < 1:3485124/65‑1 (MQ=255) ccTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGCTTGGTCGTATGACGTTTAACGGTtctc > 1:4059372/1‑65 (MQ=255) tcttctCCGAAGACCGTTATGACTTGTCTGCGCTTGGTCGTATGAAGTTCAACCGTTCTctgctg > 1:3887172/1‑65 (MQ=255) ttctCCGAAGACCGTTATGACTTGTCTGCGCTTGGTCGTATGAAGTTCAACCGTTCTCTGCTgcg > 1:1703404/1‑65 (MQ=255) aCCGTTATGACTTGTCTGCGCTTGGTCGTATGAAGTTCAACCGTTCTCTGGTGCGCGAAGAAATc > 1:4117664/1‑65 (MQ=255) aCCGTTATGACTTGTCTGCGCTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATc > 1:4245485/1‑65 (MQ=255) ccGTTATGACTTGTCTGCGCTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCg > 1:3586255/1‑65 (MQ=255) aTGACTTGTCTGCGCTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGt < 1:3838009/65‑1 (MQ=255) aTGACTTGTCTGCGCTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGt < 1:2185388/65‑1 (MQ=255) aTGACTTGTCTGCGCTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGt < 1:2727404/65‑1 (MQ=255) aTGACTTGTCTGCGCTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGt < 1:2313899/65‑1 (MQ=255) tGACTTGTCTGCGCTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGtt < 1:3289619/65‑1 (MQ=255) gACTTGTCTGCGCTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTc > 1:3342646/1‑65 (MQ=255) cagtGTCTGCGCTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTcc < 1:783390/62‑1 (MQ=255) aCTTGTCTGCGCTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTcc < 1:887354/65‑1 (MQ=255) aCTTGTCTGCGCTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTcc < 1:2930126/65‑1 (MQ=255) aCTTGTCTGCGCTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTcc < 1:2804600/65‑1 (MQ=255) cTTGTCTGCGCTTGGTCGTATGACGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCg > 1:4254836/1‑65 (MQ=255) cTTGTCTGCGCTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCg > 1:1102734/1‑65 (MQ=255) cTTGTCTGCGCTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCg > 1:3886321/1‑65 (MQ=255) cTTGTCTGCGCTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCg > 1:3803169/1‑65 (MQ=255) cTTGTCTGCGCTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCg > 1:3487322/1‑65 (MQ=255) cTTGTCTGCGCTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCg > 1:1376893/1‑65 (MQ=255) tGTCTGCGCTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGt > 1:80501/1‑65 (MQ=255) tCTGCGCTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTAt > 1:683123/1‑65 (MQ=255) gcgcTTGGTCGTATGAAGTTTAACCGTTGTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCt > 1:3523906/1‑65 (MQ=255) gcgcTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATccc > 1:4200201/1‑64 (MQ=255) | AAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCT > NC_000913/4182380‑4182503 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |