Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,390,754	G→T	A252A (GCC→GCA)	rsgA ←	ribosome small subunit‑dependent GTPase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,390,754	0	G	T	100.0%	108.7 / NA	30	A252A (GCC→GCA)	rsgA	ribosome small subunit‑dependent GTPase A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (14/16);  total (14/16)

TCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTC  >  NC_000913/4390695‑4390813                                                            |                                                            tCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGt                                                        <  1:4064753/65‑1 (MQ=255)        cTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCgtggtg                                                 <  1:2197269/65‑1 (MQ=255)         tCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGgtgt                                                <  1:1126439/65‑1 (MQ=255)         tCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGgtgt                                                <  1:1352007/65‑1 (MQ=255)         tCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGgtgt                                                <  1:4139755/65‑1 (MQ=255)         tCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGgtgt                                                <  1:332714/65‑1 (MQ=255)         tCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGgtgt                                                <  1:2804768/65‑1 (MQ=255)         tCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGgtgt                                                <  1:2650606/65‑1 (MQ=255)         tCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGgtgt                                                <  1:2047471/65‑1 (MQ=255)         tCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAACCGAGTTGTGGCCGTGgtgt                                                >  1:4121836/1‑65 (MQ=255)         gccTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGgtgt                                                <  1:2496742/64‑1 (MQ=255)          ccTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGtgtg                                               >  1:1218013/1‑65 (MQ=255)           cTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGc                                              <  1:817177/65‑1 (MQ=255)               ggAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGGGGGCGTGGTGGGCGGAc                                          >  1:2138626/1‑65 (MQ=255)               ggAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGAc                                          >  1:2087743/1‑65 (MQ=255)               ggAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGGGTGCTGAc                                          >  1:4067938/1‑65 (MQ=255)                 aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGTTGTGCTGACCg                                        >  1:2285149/1‑65 (MQ=255)                 aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGAccc                                        >  1:1791421/1‑64 (MQ=255)                 aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                        >  1:2266853/1‑65 (MQ=255)                 aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                        >  1:454338/1‑65 (MQ=255)                 aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                        >  1:2797526/1‑65 (MQ=255)                 aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                        >  1:1830508/1‑65 (MQ=255)                 aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGCGGGCCGACCg                                        >  1:226224/1‑65 (MQ=255)                 aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTCCGGCCGTGGTGGGCTGACCg                                        >  1:2891163/1‑65 (MQ=255)                   tCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAg                                      <  1:392545/65‑1 (MQ=255)                      atcacatcacCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGccc                                   >  1:3326028/1‑65 (MQ=255)                       tcacatcacCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGcccc                                  >  1:1946582/1‑65 (MQ=255)                         acatcacCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGa                                >  1:1708084/1‑65 (MQ=255)                                 gccgTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCag                        >  1:357565/1‑65 (MQ=255)                                 gccgTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCag                        >  1:290597/1‑65 (MQ=255)                                        gggAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATc                 >  1:3183301/1‑65 (MQ=255)                                                aTACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCa         >  1:356511/1‑65 (MQ=255)                                                aTACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCa         >  1:1503629/1‑65 (MQ=255)                                                  aCAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAgg       <  1:649201/65‑1 (MQ=255)                                                    aGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGATGTCAGAGTCATCGTTGGTCAGGAt     >  1:3648705/1‑65 (MQ=255)                                                      ccGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAtct   <  1:226929/65‑1 (MQ=255)                                                       cGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAtctc  <  1:216824/65‑1 (MQ=255)                                                       cGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAtctc  <  1:1621057/65‑1 (MQ=255)                                                       cGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAtctc  <  1:91380/65‑1 (MQ=255)                                                            |                                                            TCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTC  >  NC_000913/4390695‑4390813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑