| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,560,455 | +G | pseudogene (151/758 nt) | glpR ← | DNA‑binding transcriptional repressor GlpR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,560,455 | 1 | . | G | 94.7% | 57.9 / ‑2.2 | 19 | pseudogene (151/758 nt) | glpR | DNA‑binding transcriptional repressor GlpR |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base G (8/10); total (9/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01 | |||||||||||
GCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑C‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAG > NC_000913/3560392‑3560502 | gCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑cg > 1:2569810/1‑64 (MQ=255) gTGCCAGGGCGTGGAACCGGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGt < 1:4208501/65‑1 (MQ=255) ggCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTc > 1:2040420/1‑65 (MQ=255) ggCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAGGG‑CGCAGGATCAGGTTTTGCTc > 1:298856/1‑65 (MQ=255) gCGTGTTAACCGAACTGGAGGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTcc < 1:2278287/65‑1 (MQ=255) ttAACCGAGCTGGAAGGCAGCGCCGCGCCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAg < 1:969257/65‑1 (MQ=38) ttAACCGAGCTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAg < 1:2858564/65‑1 (MQ=39) ttAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAg < 1:401036/65‑1 (MQ=255) ttAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAg < 1:345551/65‑1 (MQ=255) ttAACCAGACTGGAGGGCAGCGCCGAACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAg < 1:4577168/65‑1 (MQ=37) tAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGc < 1:901256/65‑1 (MQ=255) aaCCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCt > 1:2278578/1‑65 (MQ=255) ccGAACTGGAAGGCAGCGCCGCACCGCCATGATGGTG‑CAGGATCAGGTTTTGCTCCGCCAGCTCa > 1:289609/1‑65 (MQ=255) ccGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCa > 1:1459802/1‑65 (MQ=255) ccGAACTGCAAGGCAGCGCCGCACCGCCACGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCa < 1:3276225/65‑1 (MQ=38) aCTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGa > 1:1993302/1‑65 (MQ=255) aaGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTcg > 1:2732575/1‑65 (MQ=255) gggCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTcgc < 1:4097506/64‑1 (MQ=39) aGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTcgc > 1:4580603/1‑65 (MQ=255) aGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCACAATGAGGTcgc > 1:431439/1‑65 (MQ=38) gCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCgcgg > 1:2191553/1‑65 (MQ=255) gCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCgcgg > 1:4596317/1‑65 (MQ=255) gCAGCGCCGCACCGCCATGATGG‑CGCAGGAGCAGGTTTTGCTCCGCCAGCTCCTTGAGGTcgcag > 1:1387498/1‑63 (MQ=255) cgcACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAg < 1:3803016/65‑1 (MQ=255) | GCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑C‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAG > NC_000913/3560392‑3560502 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |