| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,374,040 | 0 | C | T | 35.1% | 30.1 / 35.5 | 37 | F273F (TTC→TTT) ‡ | ycjO | putative ABC transporter membrane subunit YcjO |
| Reads supporting (aligned to +/- strand): ref base C (5/19); new base T (0/13); total (5/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.40e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.81e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCTACAAAACCGCCTTTGCCTTTAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATGGCAACAAATA > NC_000913/1373976‑1374102 | tctACAAAACCGCCTTTGCCTTTAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGTGGTCTtc > 1:1791214/1‑65 (MQ=255)tctACAAAACCGCCTTTGCCTTTAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGGGCTCTtc > 1:813493/1‑65 (MQ=255)gctACAAAACCGCCTTTGCCTTTAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGTGCTCTtc < 1:2140293/64‑1 (MQ=255) aCAAAACCGCCTTTGCCTTTAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGTGCTCTtcatc > 1:3504823/1‑65 (MQ=255) aCAAAACCGCCTTTGCCTTTAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGGGCTCTtcatc > 1:1977462/1‑65 (MQ=255) ttgcctttAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGTGCTCTTCATCATTATTTTCGCt < 1:840686/65‑1 (MQ=255) gcctttAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGt < 1:4350017/65‑1 (MQ=255) gcctttAATGATTGAGGGGGAGGCGTGTAGATCTCGGTGGT‑CGCCGTATCATTATTTTCGCTGTc < 1:2522784/65‑1 (MQ=255) gctttAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTc < 1:1522866/64‑1 (MQ=255) cctttAATGCTTTAGGAAAAGCGGCGGCGATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTc < 1:1112886/65‑1 (MQ=255) cctttAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTc < 1:186830/65‑1 (MQ=255) cctttAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTc < 1:4062751/65‑1 (MQ=255) cctttAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTc < 1:3178950/65‑1 (MQ=255) tttAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTCAt < 1:2445134/65‑1 (MQ=255) tAATGATTAGGGAAAAGGGGCGGCGATCTCGGTGGT‑CGCCGTATCATTATTTTCGCTGTCATTct < 1:3600184/65‑1 (MQ=255) aaTGATTTAGGAAAAGCGGCGGCGATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTCATTct < 1:1276180/65‑1 (MQ=255) aTGATTTAGGAAGAGGGGCGGCGATCTCGGTGGT‑CGCCGTATCATTATTTTCGCTGTCATTctgc < 1:2506311/65‑1 (MQ=255) aTTTAGAAAAAGCGGCGGCGATCTCGGTGGT‑CGCCGTATCATTATTTTCGCTGTCATTCTGCTGa < 1:4260352/65‑1 (MQ=255) ttAGGAGAAGGCGCGTCGATCTCGGTGGT‑CGCCGTATCATTATTTTCGCTGTCATTCTGCTGAcc < 1:943094/65‑1 (MQ=255) gAAAAGCGGCGGCGATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAgg < 1:907045/65‑1 (MQ=255) gAAAAGCGGCGGCGATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAgg < 1:2521374/65‑1 (MQ=255) gAAAAGCGGCGGCGATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAgg < 1:3212496/65‑1 (MQ=255) gcggcggcgATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACg < 1:2581001/65‑1 (MQ=255) tagATCTCGGTGGT‑CGCCGTATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAAc < 1:1373450/63‑1 (MQ=255) gcgATCTCGGTCGTGCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAGCGGGTGaa < 1:3741038/65‑1 (MQ=255) cgATCTCGGTGGT‑CGCCGTATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAAcc < 1:1258388/65‑1 (MQ=255) agATCTCGGTGGT‑CGCCGTATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAAcc < 1:1995763/64‑1 (MQ=255) agATCTCGGTGGT‑CGCCGTATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAAcc < 1:3809222/64‑1 (MQ=255) agATCTCGGTGGT‑CGCCGTATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAAcc < 1:2948070/64‑1 (MQ=255) agATCTCGGTGGT‑CGCCGTATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAAcc < 1:528010/64‑1 (MQ=255) agATCTCGGTGGT‑CGCCGTATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAAAGGGTGaaaa < 1:3062859/64‑3 (MQ=255) agATCTCGGTGGT‑CGCCGTATCATTATTTTCGCTGTCATTCTGCTGAACAGGAAAAGGGTGaaaa < 1:3012492/64‑3 (MQ=255) gATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAAcc < 1:2982532/65‑1 (MQ=255) gATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAAcc < 1:2418223/65‑1 (MQ=255) gATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAAcc < 1:2681582/65‑1 (MQ=255) cGGTGGTGCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAAt > 1:1968269/1‑65 (MQ=255) ggtgCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATGGca > 1:4300517/1‑65 (MQ=255) tgCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATGGcaac < 1:51684/65‑1 (MQ=255) tgCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATGGcaac > 1:4185407/1‑65 (MQ=255) gCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATGGcaaca > 1:2508423/1‑65 (MQ=255) ctctTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATGGcaacaa < 1:457000/65‑1 (MQ=255) tctTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATGGCAACaaa > 1:3618812/1‑65 (MQ=255) ttCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATGGCAACAAATa < 1:3307927/65‑1 (MQ=255) | TCTACAAAACCGCCTTTGCCTTTAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATGGCAACAAATA > NC_000913/1373976‑1374102 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |