| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,644,918 | 0 | T | G | 29.6% | 110.6 / 12.7 | 56 | T89P (ACC→CCC) | prlC | oligopeptidase A |
| Reads supporting (aligned to +/- strand): ref base T (10/28); new base G (16/0); total (28/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.52e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TACGCTTTATACAGCCCTTCATGTTGCCCTACCCAGGTGCTGTATTCCGACAGCAGCGGCAGGGTTTGTTCGTAGGCTTCACGCAGTTCCGGGCTATTTTTCACCGAGTTCAGGTGGCTGACCGG > NC_000913/3644854‑3644978 | tACGCTTTATACAGCCCTTCATGTTGCCCTACCCAGGTGCTGTATTCCGACAGCAGCGGCAGGGt > 1:4562481/1‑65 (MQ=255) gCTTTATACAGCCCTTCATGTTGCCCTACCCAGGTGCTGTATTCCGACAGCAGCGGCAGGGTTTg > 1:1407581/1‑65 (MQ=255) gCTTTATACAGCCCTTCATGTTGCCCTACCCAGGTGCTGTATTCCGACAGCAGCGGCAGGGGTTg > 1:2230060/1‑65 (MQ=255) cTTTATACAGCCCTTCATGTTGCCCTACCCAGGTGCTGTATTCCGACAGCAGCGGCAGGGTTTGt < 1:3416788/65‑1 (MQ=255) cTTTATACAGCCCTTCATGTTGCCCTACCCAGGTGCTGTATTCCGACAGCAGCGGCAGGGTTTGt < 1:4480486/65‑1 (MQ=255) cTTTATACAGCCCTTCATGTTGCCCTACCCAGGTGCTGTATTCCGACAGCAGCGGCAGGGGTTGt > 1:1170943/1‑65 (MQ=255) tttATACAGCCCTTCATGTTGCCCTACCCAGGTGCTGTATTCCGACAGCAGCGGCAGGGTTTGtt < 1:2731571/65‑1 (MQ=255) taCAGCCCTTCATGTTGCCCTACCCAGGTGCTGTATTCCGACAGCAGCGGCAGGGGTTGTTCGTa > 1:390778/1‑65 (MQ=255) aCAGCCCTTCATGTTGCCCTACCCAGGTGCTGTATTCCGACAGCAGCGGCAGGGTTTGTTCGTAg > 1:440146/1‑65 (MQ=255) aCAGCCCTTCATGTTGCCCTACCCAGGTGCTGTATTCCGACAGCAGCGGCAGGGTTTGTTCGTAg > 1:4174744/1‑65 (MQ=255) aCAGCCCTTCATGTTGCCCTACCCAGGTGCTGTATTCCGACAGCAGCGGCAGGGTTTGTTCGTAg > 1:3005088/1‑65 (MQ=255) aCAGCCCTTCATGTTGCCCTACCCAGGTGCTGTATTCCGACAGCAGCGGCAGGGTTTGTTCGTAg > 1:3462482/1‑65 (MQ=255) aCAGCCCTTCATGTTGCCCTACCCAGGTGCTGTATTCCGACAGCAGCGGCAGGGTGTGTTCGTAg > 1:3382608/1‑65 (MQ=255) aCAGCCCTTCATGTTGCCCTACCCAGGTGCTGTATTCCGACAGCAGCGGCAGGGGTTGTTCGTAg > 1:3266143/1‑65 (MQ=255) aCAGCCCTTCATGTTGCCCTACCCAGGTGCTGTATTCCGACAGCAGCGGCAGGGGTTGTTCGTAg > 1:3432725/1‑65 (MQ=255) aCAGCCCTTCATGTTGCCCTACCCAGGTGCTGTATTCCGACAGCAGCGGCAGGGCTTTTTCGGAg > 1:4446981/1‑65 (MQ=255) aCAGCCCTTCATGTTGCCCTACCCAGGTGCTGTATTCCGACAGCAGCGGCAGGGCTTGTTCGTAg > 1:1452713/1‑65 (MQ=255) acaccTTCATGTTGCCCTACCCAGGTGCTGTATTCCGACAGCAGCGGCAGGGTTTGTTCGTAGGc < 1:1084762/62‑1 (MQ=255) aGCCGTTCATGTTGCCCTACCCAGGTGCTGTATTCCGACAGCAGCGGCAGGGTTTGTTCGTAGGc < 1:854032/65‑1 (MQ=255) aGCCCTTCATGTTGCCCTACCCAGGTGCTGTATTCCGACAGCAGCGGCAGGGTTTGTTCGTAGGc < 1:2969734/65‑1 (MQ=255) aGCCCTTCATGTTGCCCTACCCAGGTGCTGTATTCCGACAGCAGCGGCAGGGTTTGTTCGTAGGc < 1:3117494/65‑1 (MQ=255) aGCCCTTCATCTTGCCCTACCCATGTGCTGTATTCCGACAGCAGCGGCAGGGTTTGTTCGTAGGc < 1:2945621/65‑1 (MQ=255) gCCCTTCATGTTGCCCTACCCAGGTGCTGTATTCCGACAGCAGCGGCAGGGTTTGTTCGTAGGCt > 1:352433/1‑65 (MQ=255) gCCCTTCATGTTGCCCTACCCAGGTGCTGTATTCCGACAGCAGCGGCAGGGGTTGTTCGTTGGCt > 1:4147448/1‑65 (MQ=255) gCCCTTCATGTTGCCCTACCCAGGTGCTGTATTCCGACAGCAGCGGCAGGGGTTGTTCGTAGGCt > 1:3849891/1‑65 (MQ=255) gCCCTTCATGTTGCCCTACCCAGGTGCTGTATTCCGACAGCAGCGGCAGGGGTTGTTCGTAGGCt > 1:3128247/1‑65 (MQ=255) ccTTCATGTTGCCCTACCCAGGTGCTGTATTCCGACAGCAGCGGCAGGGTTTGTTCGTAGGCTTc > 1:1279650/1‑65 (MQ=255) ccTTCATGTTGCCCTACCCAGGTGCTGTATTCCGACAGCAGCGGCAGGGGTTGTTCGTAGGGttt > 1:3598436/1‑64 (MQ=255) ccTTCATGTTGCCCTACCCAGGTGCTGTATTCCGACAGCAGCGGCAGGGGTTGTTCGTAGGCTTc > 1:3110289/1‑65 (MQ=255) ttCATGTTGCCCTACCCAGGTGCTGTATTCCGACAGCAGCGGCAGGGTTTGTTCGTAGGCTTCAc < 1:4120409/65‑1 (MQ=255) aTGTTGCCCTACCCAGGTGCTGTATTCCGACAGCAGCGGCAGGGTTTGTTCGTAGGCTTCACGCa < 1:2382614/65‑1 (MQ=255) tGTTGCCCTACCCAGGTGCTGTATTCCGACAGCAGCGGCAGGGGTTGTTCGTAGGCTTCACGCAg > 1:1739213/1‑65 (MQ=255) gTTGCCCTACCCAGGTGCTGTATTCCGACAGCAGCGGCAGGGTTTGTTCGTAGGCTTCACGCAGt < 1:2426720/65‑1 (MQ=255) gTTGCCCTACCCAGGTGCTGTATTCCGACAGCAGCGGCAGGGTTTGTTCGGAGGCTTCACGCAGt > 1:1530534/1‑65 (MQ=255) gTTGCCCTACCCAGGTGCTGTATTCCGACAGCAGCGGCAGGGGTTGTTCGTAGGCTTCACGCAGt > 1:171736/1‑65 (MQ=255) ccTACCCAGGTGCTGTATTCCGACAGCAGCGGCAGGGTTTGTTCGTAGGCTTCACGCAGTTCCgg > 1:2919206/1‑65 (MQ=255) ccTACCCAGGTGCTGTATTCCGACAGCAGCGGCAGGGTTTGTTCGTAGGCTTCACGCAGTTCCgg > 1:2285715/1‑65 (MQ=255) ccTACCCAGGTGCTGTATTCCGACAGCAGCGGCAGGGTTTGTTCGTAGGCTTCACGCAGGTCCgg > 1:1584743/1‑65 (MQ=255) ccTACCCAGGTGCTGTATTCCGACAGCAGCGGCAGGGGTTGTTCGTAGGCTTCCCGCCGATCCgg > 1:4068460/1‑65 (MQ=255) ccTACCCAGGTGCTGTATTCCGACAGCAGCGGCAGGGGTTGTTCGTAGGCTTCACGCAGTTCCgg > 1:390743/1‑65 (MQ=255) tACCCAGGTGCTGTATTCCGACAGCAGCGGCAGGGGTTGTTCGTAGGCTTCACGCAGTTCCGGGc > 1:901353/1‑65 (MQ=255) ccAGGTGCTGTATTCCGACAGCAGCGGCAGGGGTTGGTCGTAGGCTTCACGCAGTTCCGGGCCAt > 1:4416382/1‑65 (MQ=255) aGGTGCTTTATTCCGACAGCAGCGGCAGGGTTTGTTCGTAGGCTTCACGCAGTTCCGGGCTAttt < 1:510280/65‑1 (MQ=255) ggTGCTGTATTCCGACAGCAGCGGCAGGGTTTGGTCGTAGGCTTCACGCAGTTTCGGGGTAtttt > 1:1037729/1‑65 (MQ=255) tGCTGTATTCCGACAGCAGCGGCAGGGTTTGTTGGTAGGCTTCACGCAGTTCCGGGCTATTTTTc > 1:4311837/1‑65 (MQ=255) tGCTGTATTCCGACAGCAGCGGCAGGGTTTGTTCGTAGGCTTCACGCAGTTCCGGGCTATTTTTc > 1:273122/1‑65 (MQ=255) tGCTGTATTCCGACAGCAGCGGCAGGGTTTGTTCGTAGGCTTCACGCAGTTCCGGGCTATTTTTc > 1:1461667/1‑65 (MQ=255) tGCTGTATTCCGACAGCAGCGGCAGGGTTTGTTCGTAGGCTTCACGCAGTTCCGGGCTATTTTTc > 1:2274479/1‑65 (MQ=255) tGCTGTATTCCGACAGCAGCGGCAGGGTTTGCTCGTAGGCTTCCCGCAGGTCCGGGCGATTTTTc > 1:926689/1‑65 (MQ=255) tGCTGTATTCCGACAGCAGCGGCAGGGCTTGGTTGCAGGCTTCACGCAGTTTTGGGCTAtttttt > 1:3174603/1‑64 (MQ=255) gCTGTATTCCGACAGCAGCGGCAGGGTTTGTTCGTAGGCTTCACGCCGTTCCGGGCTATTTTTCa > 1:2998388/1‑65 (MQ=255) gCTGTATTCCGACAGCAGCGGCAGGGGTTGTTTGTAGGCTTCACGCAGTTCCGGGGTCtttttta > 1:2676062/1‑63 (MQ=255) gCTGTATTCCGACAGCAGCGGCAGGGGTTGTTCGTAGGCTTCACGCAGTTCCGGGGTATTTTTCa > 1:1246817/1‑65 (MQ=255) gCTGTATTCCGACAGCAGCGGCAGGGCTTGTTCGTAGGCTTCACGCAGTTCCGGGCTATTTTTCa > 1:409311/1‑65 (MQ=255) cTGTATTCCGACAGCAGCGGCAGGGTTTGTTCGTAGGCTTCACGCAGTTCCGGGCTATTTTTCAc < 1:4142329/65‑1 (MQ=255) tGTATTCCGACAGCAGCGGCAGGGTTTGTTCGTAGGCTTCACGCAGTTCCGGGCTATTTTTCAcc > 1:219826/1‑65 (MQ=255) tGTATTCCGACAGCAGCGGCAGGGGTTGTTCGTAGGCTTCACGCAGTTCCGGGCTATTTTTcccc > 1:4132672/1‑65 (MQ=255) tATTCCGTCAGCAGCGGCAGGGTTTGTTCGTAGGCTTCACGCAGTTCCGGGCTATTTTTCACCGa < 1:4565360/65‑1 (MQ=255) tATTCCGACAGCAGCGGCAGTGTTTGTTCGTAGGCTTCACGCAGTTCCGGGCTATTTTTCACCGa < 1:2868891/65‑1 (MQ=255) tATTCCGACAGCAGCGGCAGGGTTTGTTCGTAGGCTTCACGCAGTTCCGGGCTATTTTTCACCGa < 1:3262924/65‑1 (MQ=255) tATTCCGACAGCAGCGGCAGGGTTTGTTCGTAGGCTTCACGCAGTTCCGGGCTATTTTTCACCGa < 1:1541014/65‑1 (MQ=255) tATTCCGACAGCAGCGGCAGGGTTTGTTCGTAGGCTTCACGCAGTTCCGGGCTATTTTTCACCGa < 1:473671/65‑1 (MQ=255) tATTCCGACAGCAGCGGCAGGGTTTGTTCGTAGGCTTCACGCAGTTCCGGGCTATTTTTCACCGa < 1:4271534/65‑1 (MQ=255) tATTCCGACAGCAGCGGCAGGGTTTGTTCGTAGGCTTCACGCAGTTCCGGGCTATTTTTCACCGa < 1:3055105/65‑1 (MQ=255) aTTCCGACAGCAGCGGCAGGGCTTGTTCGTAGGGTTCACCCAGTTCCGGGCCATTTTTCACCGAg > 1:443399/1‑65 (MQ=255) ttCCGACAGCGGCGGCAGGGTTTGTTCGTAGGCTTCACGCAGTTCCGGGCTATTTTTCACCGAGt < 1:4050939/65‑1 (MQ=255) ttCCGACAGCAGCGGCAGGGTTTGTTCGTAGGCTTCACGCAGTTCCGGGCTATTTTTCGCCGAGt < 1:2026698/65‑1 (MQ=255) ttCCGACAGCAGCGGCAGGGTTTGTTCGTAGGCTTCACGCAGTTCCGGGCTATTTTTCACCGAGt < 1:3249648/65‑1 (MQ=255) ttCCGACAGCAGCGGCAGGGTTTGTTCGTAGGCTTCACGCAGTTCCGGGCTATTTTTCACCGAGt < 1:3754141/65‑1 (MQ=255) ttCCGACAGCAGCGGCAGGGTTTGTTCGTAGGCTTCACGCAGTTCCGGGCTATTTTTCACCGAGt < 1:3753482/65‑1 (MQ=255) ttCCGACAGCAGCGGCAGGGTTTGTTCGTAGGCTTCACGCAGTTCCGGGCTATTTTTCACCGAGt < 1:2969990/65‑1 (MQ=255) tCCGACAGCAGCGGCAGGGTTTGTTTGTAGGTCTCAAGCCGTTTCGGGCTCTTTTTCACCCAgat > 1:1047508/1‑63 (MQ=255) tCCGACAGCAGCGGCAGGGGTTGTTCGTAGGCTTCACGCAGTTTCGGGCTATTTTTCACCGAGtt > 1:2564627/1‑65 (MQ=255) tCCGACAGCAGCGGCAGGGGTTGTTCGTAGGCTTCACGCAGTTCCGGGCTATTTTTCACCGAGtt > 1:707404/1‑65 (MQ=255) tCCGACAGCAGCGGCAGGGCTTGTTCGTAGGCTTCACGCAGTTCCGGGCTATTTTTCACCGAGtt > 1:596141/1‑65 (MQ=255) ccGACAGCAGCGGCAGGGGTTGTTCGTAGGCTTCACGCAGTTCCGGGCTATTTTTCACCGAGTTc > 1:843945/1‑65 (MQ=255) cagcagCGGCAGGGTTTGTTCGTAGGCTTCACGCAGTTCCGGGCTATTTTTCACCGAGTTCAGGt < 1:154371/65‑1 (MQ=255) agcagcGGCAGGGTTTATTCGTAGGCTTCACGCAGTTCCGGGCTATTTTTCACCGAGTTCAGGTg < 1:2656450/65‑1 (MQ=255) gcagcGGCAGGGTTTGTTCGTAGGCTTCACGCAGTTCCGGGCTATTTTTCACCGAGTTCAGGTgg < 1:3561836/65‑1 (MQ=255) cAGGGGTTGTTCGTAGGCTTCACGCAGTTCCGGGCTATTTTTCACCGAGTTCAGGTGGCTGACCg > 1:235856/1‑65 (MQ=255) aGGGTTTGTTCGTAGGCTTCACGCAGTTCCGGGCTATTTTTCGCCGAGTTCAGGTGGCTGACCgg < 1:3621370/65‑1 (MQ=255) | TACGCTTTATACAGCCCTTCATGTTGCCCTACCCAGGTGCTGTATTCCGACAGCAGCGGCAGGGTTTGTTCGTAGGCTTCACGCAGTTCCGGGCTATTTTTCACCGAGTTCAGGTGGCTGACCGG > NC_000913/3644854‑3644978 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |