Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,390,754	G→T	A252A (GCC→GCA)	rsgA ←	ribosome small subunit‑dependent GTPase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,390,754	0	G	T	100.0%	110.5 / NA	31	A252A (GCC→GCA)	rsgA	ribosome small subunit‑dependent GTPase A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (25/6);  total (25/6)

ACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTC  >  NC_000913/4390693‑4390813                                                              |                                                            aCTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAgctgcg                                                          >  1:3225392/1‑65 (MQ=255)  cTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGctgcgg                                                         >  1:3194364/1‑65 (MQ=255)  cTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGctgcgg                                                         >  1:229611/1‑65 (MQ=255)   tCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGt                                                        <  1:2682167/65‑1 (MQ=255)         aCTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCgtggt                                                  >  1:229627/1‑65 (MQ=255)           tcgtGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGgtgt                                                <  1:2353805/62‑1 (MQ=255)           tCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATGCAGCCGAGCTGCGGTCGTGgtgt                                                <  1:153450/65‑1 (MQ=255)           gccTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGgtgt                                                <  1:405961/64‑1 (MQ=255)            ccTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGtgtg                                               >  1:4331960/1‑65 (MQ=255)               ggggAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTg                                            >  1:4365967/1‑65 (MQ=255)               ggggAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTg                                            >  1:4187523/1‑65 (MQ=255)                   aGTCAATCACATCACCGCCGTGCGGGCAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                        >  1:427851/1‑65 (MQ=255)                   aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGGGGTCGTGGCGTGCTGCCCg                                        >  1:2941018/1‑65 (MQ=255)                   aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGActg                                        >  1:2963310/1‑63 (MQ=255)                   aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                        >  1:3480167/1‑65 (MQ=255)                   aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                        >  1:4259836/1‑65 (MQ=255)                   aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                        >  1:48615/1‑65 (MQ=255)                   aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                        >  1:1214537/1‑65 (MQ=255)                   aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                        >  1:2962087/1‑65 (MQ=255)                   aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                        >  1:735715/1‑65 (MQ=255)                   aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                        >  1:1572203/1‑65 (MQ=255)                   aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                        >  1:2366089/1‑65 (MQ=255)                   aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                        >  1:1851899/1‑65 (MQ=255)                   aGTCAATCACATCACCGCCGTGCGGGAAGCGATACAGCCGAGCTGCGGTCGTGGTGTGCTGAGCg                                        >  1:3026771/1‑65 (MQ=255)                           acatcacCGCCGTGCGGGGAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCGGa                                >  1:177822/1‑65 (MQ=255)                           acatcacCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGCCGTGGTGTGCTGACCGAGCCCCGa                                >  1:4310167/1‑65 (MQ=255)                            catcccCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAg                               <  1:2464545/65‑1 (MQ=255)                                   gccgTGCGGGAAGTGATACGGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCag                        >  1:2120706/1‑65 (MQ=255)                                   gccgTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCag                        >  1:612126/1‑65 (MQ=255)                                   gccgTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCag                        >  1:3863430/1‑65 (MQ=255)                                        gCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCCGAGcta                   >  1:1963372/1‑65 (MQ=255)                                        gCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGata                   >  1:4271886/1‑65 (MQ=255)                                              aGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGCGATATCGCTg             >  1:1272207/1‑65 (MQ=255)                                              aGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGCTATCGTTg             >  1:1250141/1‑65 (MQ=255)                                              aGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTg             >  1:408457/1‑65 (MQ=255)                                              aGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTg             >  1:3737103/1‑65 (MQ=255)                                               gtaaTACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTgg            <  1:3247610/62‑1 (MQ=255)                                                    aCAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGCGTTGTCAGAGATATCGTTGGTCacg       >  1:2526196/1‑63 (MQ=255)                                                    aCAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAgg       <  1:4515264/65‑1 (MQ=255)                                                      aGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAt     >  1:2517148/1‑65 (MQ=255)                                                         cGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAtctc  <  1:2375757/65‑1 (MQ=255)                                                         cGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAtctc  >  1:2880205/1‑65 (MQ=255)                                                              |                                                            ACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTC  >  NC_000913/4390693‑4390813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑