BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,619,325	G→A	G69E (GGA→GAA)	marR →	DNA‑binding transcriptional repressor MarR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,619,325	0	G	A	100.0%	112.9 / NA	31	G69E (GGA→GAA)	marR	DNA‑binding transcriptional repressor MarR
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (14/17); total (14/17)

ATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTT  >  NC_000913/1619264‑1619379

atgcGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGAAGc                                                     <  1:468596/62‑1 (MQ=255)

aTCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGAAGc                                                     <  1:1389013/65‑1 (MQ=255)

aTCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGAAGc                                                     <  1:3207608/65‑1 (MQ=255)

  ccGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGAAGCAc                                                   >  1:496351/1‑65 (MQ=255)

  ccGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGCATTGTCGGTCGACCTGGAAGCAc                                                   >  1:3096155/1‑65 (MQ=255)

  ccGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGAATTGTCGGTCGACCTGGAAGCAc                                                   >  1:97719/1‑65 (MQ=255)

            gcgTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGAAGCACTGACCCGTAt                                         <  1:1324049/65‑1 (MQ=255)

            gcgTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGAAGCACTGACCCGTAt                                         <  1:4031169/65‑1 (MQ=255)

            gcgTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGAAGCACTGACCCGTAt                                         <  1:277739/65‑1 (MQ=255)

            gcgTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGAAGCACTGACCCGTAt                                         <  1:4271069/65‑1 (MQ=255)

                gtATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGAAGCACTGACCCGTATGCTg                                     <  1:1640203/65‑1 (MQ=255)

                 tATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGAAGCACTGACCCGTATGCTgg                                    <  1:1425381/65‑1 (MQ=255)

                   ttACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGAAGCACTGACTCGTATGCCggct                                  >  1:644440/1‑63 (MQ=255)

                   ttACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGAAGCACTGACCCGTATGCCGGAt                                  >  1:3338968/1‑65 (MQ=255)

                   ttACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGAAGCACTGACCCGTATGCCGGAt                                  >  1:804830/1‑65 (MQ=255)

                   ttACTCCGGTTGAACTGAAAAAGGTATTGTCGGCCGACCTGGAAGCACTGCCCCGTATGCTGGAt                                  >  1:254017/1‑65 (MQ=255)

                     aCTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGAAGCACTGACCCGTATGCTGGATCg                                >  1:4051943/1‑65 (MQ=255)

                     aCTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGAAGCACTGACCCGTATGCTGGATCg                                >  1:2843963/1‑65 (MQ=255)

                     aCTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGAAGCACTGACCCGTATGCTGGATCg                                <  1:2785163/65‑1 (MQ=255)

                     aCTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGAAGCACTGACCCGTATGCTGGATCg                                >  1:768758/1‑65 (MQ=255)

                     aCTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGAAGCACTGACCCGTATGCTGGATCg                                <  1:2693117/65‑1 (MQ=255)

                       tCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGAAGCACTGACCCGTATGCTGGATCGcc                              >  1:505399/1‑65 (MQ=255)

                          ggTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGAAGCACTGACCCGTATGCTGGATCGCCTgg                           <  1:2830077/65‑1 (MQ=255)

                            ttGAACTGAAAAAGGTATTGTCGGTCGACCTGGAAGCACTGACCCGTATGCTGGATCGCCTGGTc                         >  1:3339628/1‑65 (MQ=255)

                               aaCTGAAAAAGGTATTGTCGGTCGACCTGGAAGCACTGACCCGTATGCTGGATCGCCTGGTCTGt                      <  1:436632/65‑1 (MQ=255)

                               aaCTGAAAAAGGTATTGTCGGTCGACCTGGAAGCACTGACCCGTATGCTGGATCGCCTGGTCTGt                      <  1:1122031/65‑1 (MQ=255)

                                aCTGAAAAAGGTATTGTCGGTCGACCTGGAAGCACTGACCCGTATGCTGGATCGCCTGGCCTGTa                     >  1:1270307/1‑65 (MQ=255)

                                   gAAAAAGGTATTGTCGGTCGACCTGGAAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAg                  <  1:3381500/65‑1 (MQ=255)

                                    aaaaaGGTATTGTCGGTCGACCTGGAAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAgg                 <  1:2747829/65‑1 (MQ=255)

                                       aaGGTATTGTCGGTCGACCTGGAAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTg              >  1:876322/1‑65 (MQ=255)

                                          gTATTGTCGGTCGACCTGGAAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGt           <  1:3690484/65‑1 (MQ=255)

                                               gtcggtcgACCTGGAAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGaaa      >  1:27822/1‑65 (MQ=255)

                                                  ggtcgACCTGGAAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGt   <  1:3895751/65‑1 (MQ=255)

                                                   gtcgACCTGGAAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGtt  >  1:3377850/1‑65 (MQ=255)

ATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTT  >  NC_000913/1619264‑1619379

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`