Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,711,7960AC32.4% 53.5 / 21.7 34T92P (ACC→CCC) nthendonuclease III
Reads supporting (aligned to +/- strand):  ref base A (23/0);  new base C (0/11);  total (23/11)
Fisher's exact test for biased strand distribution p-value = 3.50e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGC  >  NC_000913/1711730‑1711860
                                                                  |                                                                
gAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAAc                                                                 >  1:3767307/1‑68 (MQ=255)
gAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAAc                                                                 >  1:517838/1‑68 (MQ=255)
  aGGGGTGAAAACCTATATCAAAACGATTGGGCTTTTTAACAGCAAAGCAGAAAATATCATCAAAACCt                                                               >  1:3183738/1‑68 (MQ=255)
  aGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCt                                                               >  1:1157780/1‑68 (MQ=255)
  aGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCt                                                               >  1:291204/1‑68 (MQ=255)
  aGGGGAGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCt                                                               >  1:2404918/1‑68 (MQ=255)
   ggggTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTg                                                              >  1:3864964/1‑68 (MQ=255)
            aCCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGANAATATCATCAAAACCTGCCGTATCtt                                                     >  1:3258355/1‑68 (MQ=255)
            aCCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCtt                                                     >  1:877057/1‑68 (MQ=255)
            aCCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCtt                                                     >  1:1996177/1‑68 (MQ=255)
            aCCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCtt                                                     >  1:192127/1‑68 (MQ=255)
             ccTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATccct                                                    >  1:2879034/1‑65 (MQ=255)
             ccTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTg                                                    >  1:3729431/1‑68 (MQ=255)
             ccTATATCAAAACGATTGGGCTTTATAACAGCAAAGAAAAAAATATCATCAAACCCTGCCGTATCTTg                                                    <  1:3713949/68‑1 (MQ=255)
             ccTATAAAAAAACGATTGGGCTTTATAACAGAAAAGCAAAAAATATCATAAAACCCTGCCGTATCTTg                                                    <  1:1378295/68‑1 (MQ=255)
                  ataaaaaCGATTGGGTTTAATAACAGAAAAGCAAAAAATATCATCAAACCCTGCCGTATCTTGCTGGa                                               <  1:3909060/65‑1 (MQ=255)
                  atCAAAACGATTGGGTTTTAAAACAGAAAAGAAAAAAATATCATAAAACCCTGCCGTATCTTGCTGGa                                               <  1:1484486/68‑1 (MQ=255)
                  atCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGa                                               >  1:3024833/1‑68 (MQ=255)
                   tCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGag                                              >  1:2984978/1‑68 (MQ=255)
                    cAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGagc                                             >  1:2739187/1‑68 (MQ=255)
                       ggcGATTGGGTTTTATAACAAAAAAGAAAAAAATATCATCAAACCCTGCCGTATCTTGCTGGagcagc                                          <  1:1383602/66‑1 (MQ=255)
                       aaggATGGGGATTAAAAACGGCAAAGAAAAAAATAAAAAAAAACCCTGCCGTATCTTGCTGGagcagc                                          <  1:921164/65‑1 (MQ=255)
                       aaCGTTTGGTTTTTAAAACAGAAAAGAAAAAAATATCATCAAACCCTGCCGTATCTTGCTGGagcagc                                          <  1:1839655/68‑1 (MQ=255)
                       aaCGATTGTTTTTTATAACGACAAAGAAAAAAACTTCAACAAACCCTGCCGTATCTTGCTGGagcagc                                          <  1:3700276/68‑1 (MQ=255)
                         cGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCAt                                        >  1:1710818/1‑68 (MQ=255)
                             tGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATg                                    >  1:1055585/1‑68 (MQ=255)
                                gCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCg                                 >  1:2304429/1‑68 (MQ=255)
                                    aaaaaCAGAAAAGCAAAAAATATCATCAAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGt                             <  1:2157845/65‑1 (MQ=255)
                                     ataACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGtt                            >  1:2851000/1‑68 (MQ=255)
                                       aaCAGCAAAGAAAAAAATATCATCAAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTcc                          <  1:309878/68‑1 (MQ=255)
                                       aaCAGAAAAGCAAAAAATATCATCAAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTcc                          <  1:354918/68‑1 (MQ=255)
                                        aCAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCg                         >  1:1837055/1‑68 (MQ=255)
                                             aaaGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGCTCCGGAAGa                    >  1:2218366/1‑68 (MQ=255)
                                              aaGCAGAAAATATCATAAACCCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGAt                   <  1:3852493/68‑1 (MQ=255)
                                                 caaaaaaTATCATAAAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGt                <  1:2329409/65‑1 (MQ=255)
                                                 aaaaaaaTATAATAAAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGt                <  1:1622431/65‑1 (MQ=255)
                                                       atatCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTgcg          >  1:2795779/1‑68 (MQ=255)
                                                       atatCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTgcg          >  1:3811889/1‑68 (MQ=255)
                                                         atcatcAAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCt        <  1:1084258/68‑1 (MQ=255)
                                                          tcaacaAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCtt       <  1:2262542/68‑1 (MQ=255)
                                                               aaacCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGc  <  1:2020236/68‑1 (MQ=255)
                                                               aaacCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGc  <  1:1415945/68‑1 (MQ=255)
                                                                  |                                                                
GAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGC  >  NC_000913/1711730‑1711860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑