Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,390,754	G→T	A252A (GCC→GCA)	rsgA ←	ribosome small subunit‑dependent GTPase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,390,754	0	G	T	100.0%	86.3 / NA	25	A252A (GCC→GCA)	rsgA	ribosome small subunit‑dependent GTPase A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (14/11);  total (14/11)

TCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCT  >  NC_000913/4390695‑4390814                                                            |                                                             tCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCgt                                                      >  1:3961342/1‑68 (MQ=255) tCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCgt                                                      <  1:2415079/68‑1 (MQ=255) tCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCgt                                                      <  1:3379334/68‑1 (MQ=255)      cACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGgtgt                                                 <  1:1242000/68‑1 (MQ=255)      cACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGgtgt                                                 <  1:2430614/68‑1 (MQ=255)      cACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGgtgt                                                 <  1:3044185/68‑1 (MQ=255)           cTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGGGGGCTGa                                            >  1:33927/1‑68 (MQ=255)            tGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGAc                                           <  1:326587/68‑1 (MQ=255)             ggggAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGAcc                                          >  1:3231291/1‑68 (MQ=255)                gAGTCATTCACATCCCCGCCGTGCGGGATGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAg                                       <  1:3407384/68‑1 (MQ=255)                gAGTCAATCCCACCCCCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAg                                       <  1:2669457/68‑1 (MQ=255)                 aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGANCTGCGGTCGTGGTGTGCTGACGGAGc                                      >  1:3257326/1‑68 (MQ=255)                 aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACGGAGc                                      >  1:776234/1‑68 (MQ=255)                 aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGc                                      >  1:2630869/1‑68 (MQ=255)                 aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGc                                      >  1:3489020/1‑68 (MQ=255)                 aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCCTGGTTTGCTGACCGAGc                                      >  1:2518770/1‑68 (MQ=255)                               ccgccgTGCGGGAAGTGATACAGCCGGGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCaga                        <  1:768449/68‑1 (MQ=255)                               ccgccgTGCGGGAAGTGATACAGCCGAGCTGGCGTCGTGGGGTGCTGACCGAGCCCCGAGTTGTCaga                        <  1:3192681/68‑1 (MQ=255)                                 gccgTGCGGGAAGTGATACAGCCGAGCTGCGGTCGCGGTGTGCTGACCGAGCCCCGAGTTGTCagagc                      >  1:3178691/1‑67 (MQ=255)                                   cgTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGCGCCCCGAGTTGTCACAGata                    >  1:2081246/1‑68 (MQ=255)                                   cgTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGata                    >  1:1797587/1‑68 (MQ=255)                                   cgTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGata                    >  1:1528817/1‑68 (MQ=255)                                     tgggggAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGNTATATc                  <  1:2452453/65‑1 (MQ=255)                                     tGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATc                  <  1:3650771/68‑1 (MQ=255)                                        gggAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGtt               >  1:1152557/1‑68 (MQ=255)                                             ttGAGACAGCCGGGCTGCGGTCGTGATGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCa          <  1:747474/67‑1 (MQ=255)                                               gATGCAGCCGAGCTGCGGTCGTGGTGTGCTGCCCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAgg        <  1:2600477/68‑1 (MQ=255)                                                  aCAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAtc     >  1:2532033/1‑68 (MQ=255)                                                  aCAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAtc     >  1:4010223/1‑68 (MQ=255)                                                    aGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAtctc   >  1:1174220/1‑68 (MQ=255)                                                    aGCCGAGCTGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAtctc   >  1:428058/1‑68 (MQ=255)                                                     gCCGAGCTGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATctct  >  1:4019152/1‑68 (MQ=255)                                                            |                                                             TCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCT  >  NC_000913/4390695‑4390814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑