Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,619,325	G→A	G69E (GGA→GAA)	marR →	DNA‑binding transcriptional repressor MarR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,619,325	0	G	A	100.0%	143.8 / NA	39	G69E (GGA→GAA)	marR	DNA‑binding transcriptional repressor MarR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/19);  total (20/19)

TCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGG‑ATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCG  >  NC_000913/1619261‑1619389                                                                 |                                                                  tctATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGAAGc                                                                >  1:1053492/1‑68 (MQ=255) tctATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGAAGc                                                                <  1:3231979/68‑1 (MQ=255) tctATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGAAGc                                                                <  1:2975900/68‑1 (MQ=255) tctATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGAAGc                                                                >  1:3772801/1‑68 (MQ=255) tctATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGAAGc                                                                <  1:746707/68‑1 (MQ=255) tctATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGAAGc                                                                <  1:946480/68‑1 (MQ=255)      ccGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGAAGCACTGa                                                           >  1:1433545/1‑68 (MQ=255)      ccGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGAAGCACTGa                                                           >  1:508150/1‑68 (MQ=255)      ccGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGAAGCACTGa                                                           >  1:2277370/1‑68 (MQ=255)        gCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGAAGCACTGAcc                                                         <  1:2255406/68‑1 (MQ=255)        gCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGAAGCACTGAcc                                                         <  1:2409366/68‑1 (MQ=255)         cTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGAAGCACTGAccc                                                        >  1:3399588/1‑68 (MQ=255)             gcggcgTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGAAGCACTGACCCGTAt                                                    <  1:2005536/68‑1 (MQ=255)                  gtgtATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGAAGCACTGACCCGTATGCTgg                                               <  1:4056604/68‑1 (MQ=255)                  aggtATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGAAGCACTGACCCGTATGCTgg                                               <  1:2967413/66‑1 (MQ=255)                  agatATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGAAGCACTGACCCGTATGCTgg                                               <  1:3716726/65‑1 (MQ=255)                    gtATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGAAGCACTGACCCGTATGCTGG‑At                                            >  1:3986206/1‑68 (MQ=255)                      aTTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGAAGCACTGACCCGTATGCTGG‑ATCg                                          <  1:1317044/68‑1 (MQ=255)                      aTTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGAAGCACTGACCCGTATGCTGG‑ATCg                                          <  1:877930/68‑1 (MQ=255)                      aTTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGAAGCACTGACCCGTATGCNTG‑ATCg                                          <  1:2448751/68‑1 (MQ=255)                         aCTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGAAGCACTGACCCGTATGCTGG‑ATCGCCt                                       >  1:2594521/1‑68 (MQ=255)                         aCTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGAAGCACTGACCCGTATGCTGG‑ATCGCCt                                       >  1:2606188/1‑68 (MQ=255)                         aCTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGAAGCACTGACCCGTATGCTGG‑ATCGCCt                                       >  1:2936945/1‑68 (MQ=255)                         aCTCCGGTTGAACTGAAAAAGGTATTGTCAGTCGACCTGGAAGCACTGACCCGTATGCTGG‑ATCGCCt                                       >  1:1466666/1‑68 (MQ=255)                           tCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGAAGCACTGACCCGTATGCTGG‑ATCGCCTgg                                     >  1:3509024/1‑68 (MQ=255)                           tCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGAAGCACTGACCCGTATGCTGG‑ATCGCCTgg                                     <  1:517488/68‑1 (MQ=255)                           tCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGAAGCACTGACCCGTATGCTGG‑ATCGCCTgg                                     >  1:1106671/1‑68 (MQ=255)                           tCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGAAGCACTGACCCGTATGCTGG‑ATCGCCTgg                                     <  1:2139433/68‑1 (MQ=255)                           tCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGAAGCACTGACCCGTATGCTGG‑ATCGCCTgg                                     >  1:1143986/1‑68 (MQ=255)                            ccGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGAAGCACTGACCCGTATGCTGG‑ATCGCCTGGt                                    <  1:3644701/68‑1 (MQ=255)                                ttGAACTGAAAAAGGTATTGTCGGTCGACCTGGAAGCACTGACCCGTATGCTGG‑ATCGCCTGGTCTGt                                >  1:3176310/1‑68 (MQ=255)                                ttGAACTGAAAAAGGTATTGTCGGTCGACCTGGAAGCACTGACCCGTATGCTGG‑ATCGCCTGGTCTGt                                <  1:1447920/68‑1 (MQ=255)                                 tGAACTGAAAAAGGTATTGTCGGTCGACCTGGAAGCACTGACCCGTATGCTGG‑ATCGCCTGGTCTGTa                               >  1:469974/1‑68 (MQ=255)                                    aCTGAAAAAGGTATTGTCGGTCGACCTGGAAGCACTGACCCGTATGCTGG‑ATCGCCTGGTCTGTAAAg                            >  1:2319260/1‑68 (MQ=255)                                           aaGGTATTGTCGGTCGACCTGGAAGCACTGACCCGTATGCTGG‑ATCGCCTGGTCTGTAAAGGCTGGGt                     <  1:1812411/68‑1 (MQ=255)                                              gTATTGTCGGTCGACCTGGAAGCACTGACCCGTATGCTGG‑ATCGCCTGGTCTGTAAAGGCTGGGGGGa                  >  1:2432868/1‑68 (MQ=255)                                              gTATTGTCGGTCGACCTGGAAGCACTGACCCGTATGCTGG‑ATCGCCTGGTCTGTAAAGGCTGGGGGGa                  >  1:2362747/1‑68 (MQ=255)                                                   gtcggtcgACCTGGAAGCACTGACCCGTATGCTGG‑ATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGt             >  1:1702873/1‑68 (MQ=255)                                                    tcggtcgACCTGGAAGCACTGACCCGTATGCTGG‑ATCGCCTGGTCTGTAAAGGCTGGGGGGAAAgggt            >  1:1720371/1‑66 (MQ=255)                                                       gtcgACCTGGAAGCACTGACCCGTATGCCGGCTTCCGCTTG‑ATAAAAAGGCTGGGTGGAAAGGTTGcc         >  1:111575/1‑68 (MQ=255)                                                            ccTGGAAGCACTGACCCGTATGCTGG‑ATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAAcc    <  1:988108/68‑1 (MQ=255)                                                              tGGAAGCACTGACCCGTATGCTGG‑ATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCg  <  1:2930894/68‑1 (MQ=255)                                                              tGGAAGCACTGACCCGTATGCTGG‑ATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCg  <  1:3540828/68‑1 (MQ=255)                                                                 |                                                                  TCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGG‑ATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCG  >  NC_000913/1619261‑1619389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑