| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,374,040 | 0 | C | T | 32.1% | 24.9 / 22.7 | 28 | F273F (TTC→TTT) ‡ | ycjO | putative ABC transporter membrane subunit YcjO |
| Reads supporting (aligned to +/- strand): ref base C (9/10); new base T (0/9); total (9/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.61e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.29e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACCGCCTTTGCCTTTAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATGGCAACAAA > NC_000913/1373984‑1374100 | aCCGCCTTTGCCTTTAATGATTTAGGAAAAGCGGCGGCGATCTCCGGGGTGCTCTTCATCatttt > 1:1865104/1‑63 (MQ=255)aCCGCCTTTGCCTTTAATGATTTAGGAAAAGCGGCGGCCAGCTTGGTGGGGGTCTTCATCattat > 1:649825/1‑65 (MQ=255) gcctttgcctttAATGATTTAGGAAAAGCGGCGGCGATCTCGGCGGTGCTCTTCATCATTAtttt > 1:1400406/1‑65 (MQ=255) tgcttttAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGT‑CGCCGTATCATTATTTTCGCTGt < 1:1755169/65‑1 (MQ=255) cctttAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTc < 1:2955138/65‑1 (MQ=255) tttAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTCAt < 1:2468439/65‑1 (MQ=255) aTTTAGGAAAAGCGGCGGAGATCTCGGTGGT‑CGCCGTATCATTATTTTCGCTGTCATTCTGCTGa < 1:496659/65‑1 (MQ=255) tttAGGAAAAGCGGCGGCGATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTCATTCTGCTGa < 1:2848552/65‑1 (MQ=255) ggAAAAGGCGTGTCGATCTCGGTGGT‑CGCCGTATCATTATTTTCGCTGTCATTCTGCTGACCAgg < 1:1473200/65‑1 (MQ=255) ggAAAAGCGGCGGCGATCTCGGTGGT‑CGCCGTATCATTATTTTCGCTGTCATTCTGCTGACCAgg < 1:2804024/65‑1 (MQ=255) gAAAAGCGGCGGCGATCTCGGTGGTTCTCCGCATCATTATTTTCGCTGTCATTCTGCTGACCAgg < 1:347420/65‑1 (MQ=255) gAAAAGCGGCGGCGATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAgg < 1:769098/65‑1 (MQ=255) gAAAAGCGGCGGCGATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAgg < 1:1652054/65‑1 (MQ=255) gAAAAGCGGCGGCGATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAgg < 1:2955208/65‑1 (MQ=255) ggcggcgATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACggg < 1:2028739/65‑1 (MQ=255) tagATCTCGGTGGT‑CGCCGTATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAAc < 1:380128/63‑1 (MQ=255) gcgATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGaa < 1:546826/65‑1 (MQ=255) cgATCTCGGTGGT‑CGCCGTATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAAcc < 1:7319/65‑1 (MQ=255) cgATCTCGGTGGT‑CGCCGTATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAAcc < 1:2586655/65‑1 (MQ=255) agATCTCGGTGGT‑CGCCGTATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAAcc < 1:2977990/64‑1 (MQ=255) agATCTCGGTGGT‑CGCCGTATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAAcc < 1:2853750/64‑1 (MQ=255) gATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAAcc < 1:1201442/65‑1 (MQ=255) aTCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCt < 1:3687247/65‑1 (MQ=255) ggtgCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATGGca > 1:3368311/1‑65 (MQ=255) ggtgCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATGGca > 1:200063/1‑65 (MQ=255) ggtgCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATGGca > 1:2230211/1‑65 (MQ=255) tctTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATGGCAACaaa > 1:1902265/1‑65 (MQ=255) tctTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATGGCAACaaa > 1:2469201/1‑65 (MQ=255) tctTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATGGCAACaaa > 1:2021057/1‑65 (MQ=255) tctTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATGGCAACaaa > 1:618299/1‑65 (MQ=255) tctTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATGGCAACaaa > 1:1477023/1‑65 (MQ=255) tctTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATGGCAACaaa > 1:2197242/1‑65 (MQ=255) | ACCGCCTTTGCCTTTAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATGGCAACAAA > NC_000913/1373984‑1374100 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |