| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,794,787 | 0 | C | T | 27.8% | 39.3 / 19.3 | 36 | P179S (CCG→TCG) | gabP | 4‑aminobutyrate:H(+) symporter |
| Reads supporting (aligned to +/- strand): ref base C (13/13); new base T (10/0); total (23/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.85e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.66e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTGCAAAGTCATCGCTATCCTGGCCTTTATTTTCCTTGGTGCAGTCGCAATTAGCGGTTTTTACCCGTATGCCGAAGTGAGCGGGATCTCAAGATTGTGGGATAGCGGCGGCTTTATGCCCAACGGT > NC_000913/2794723‑2794849 | gtgCAAAGTCATCGCTATCCTGGCCTTTATTTTCCTTGGTGCAGTCGCAATTAGCGGTTTTTAcc < 1:3688993/65‑1 (MQ=255)gtgCAAAGTCATCGCTATCCTGGCCTTTATTTTCCTTGGTGCAGTCGCAATTAGCGGTTTTTAcc < 1:1827662/65‑1 (MQ=255) gCAAAGTCATCGCTATCCTGGCCTTTATTTTCCTTGGTGCAGTCGCAATTAGCGGTTTTTACTCg > 1:122736/1‑65 (MQ=255) gCAAAGTCATCGCTATCCTGGCCTTTATTTTCCTTGGTGCAGTCGCAATTAGCGGTTTTTACTCg > 1:427678/1‑65 (MQ=255) cAAAGTCATCGCTATCCTGGCCTTTATTTTCCTTGGTGCAGTCGCAATTAGCGGTTTTTACCCGt < 1:2435340/65‑1 (MQ=255) gTCATCTCTATCCTGGCCTTTATTTTCCTTGGTGCAGTCGCAATTAGCGGTTTTTACTCGTATga > 1:2225595/1‑64 (MQ=255) gTCATCGCTATCCTGGCCTTTATTTTCCTTGGTGCAGTCGCAATTAGCGGTTTTTACTCGTATGc > 1:1176554/1‑65 (MQ=255) gTCATCGCTATCCTGGCCTTTATTTTCCTTGGTGCAGTCGCAATTAGCGGTTTTTACTCGTATGc > 1:1457100/1‑65 (MQ=255) gTCATCGCTATCCTGGCCTTTATTTTCCTTGGTGCAGTCGCAATTAGCGGTTTTTACTCGTATGc > 1:918594/1‑65 (MQ=255) gTCATCGCTATCCTGGCCTTTATTTTCCTTGGTGCAGTCGCAATTAGCGGTTTTTACTCGTATGc > 1:127192/1‑65 (MQ=255) gTCATCGCTATCCTGGCCTTTATTTTCCTTGGTGCAGTCGCAATTAGCGGTTTTTACTCGTATGc > 1:11841/1‑65 (MQ=255) gTCATCGCTATCCTGGCCTTTATTTTCCTTGGTGCAGTCGCAATTAGCGGTTTTTACCCGTATGc > 1:822976/1‑65 (MQ=255) gTCATCGCTATCCTGGCCTTTATTTTCCTTGGTGCAGTCGCAATTAGCGGTTTTTACCCGTATGc > 1:749831/1‑65 (MQ=255) gTCATCGCTATCCTGGCCTTTATTTTCCTTGGTGCAGTCGCAATTAGCGGTTTTTACCCGTATGc > 1:1591706/1‑65 (MQ=255) gTCATCGCTATCCTGGCCTTTATTTTCCTTGGTGCAGTCGCAATTAGCGGTTTTTACCCGTATGc > 1:3745549/1‑65 (MQ=255) gTCATCGCTATCCTGGCCTTTATTTTCCTTGGTGCAGTCGCAATTAGCGGTTTTTACCCGTATGc > 1:1726426/1‑65 (MQ=255) gTCATCGCTATCCTGGCCTTTATTTTCCTTGGTGCAGTCGCAATTAGCGGTTTTTACCCGTATGc > 1:2290700/1‑65 (MQ=255) tCGCTATCCTGACCTTTATTTTCCTTGGTGCAGTCGCAATTAGCGCTTTTTACCCGTATGCCGaa < 1:3741150/65‑1 (MQ=255) ggCCTTTATTTTCCTTGGTGCAGTCGCAATTAGCGGTTTTTACCCGTATGCCGAAGTGAGCGGGa < 1:963230/65‑1 (MQ=255) ccTTTATTTTCCTTGGTGCAGTCGCAATTAGCGGTTTTTACTCGTATGCCGTATTCAGCTGGAtc > 1:3045107/1‑65 (MQ=255) ccTTTATTTTCCTTGGTGCAGTCGCAATTAGCGGTTTTTACTCGTATGCCGACGTGAGCGGGAtc > 1:168148/1‑65 (MQ=255) aTTTTCCTTGGTGCAGTCGCAATTAGCGGTTTTTACCCGTATGCCGAAGTGAGCGGGATCTCAAg < 1:2879883/65‑1 (MQ=255) ttttCCTTGGTGCAGTCGCAATTAGCGGTTTTTACCCGTATGCCGAAGTGAGCGGGATCTCAAGa < 1:1586656/65‑1 (MQ=255) tGGTGCAGTCGCAATTAGCGGTTTTTACCCGTATGCCGAAGTGAGCGGGATCTCAAGATTGTggg < 1:3443186/65‑1 (MQ=255) gaaGTCGCAATTAGCGGTTTTTACCCGTATGCCGAAGTGAGCGGGATCTCAAGATTGTGGGATAg > 1:1006110/3‑65 (MQ=255) gCAGTCGCAATTAGCGGTTTTTACCCGTATGCCGAATTGTGCTTGAACTCAAGATTGTGGGATAg > 1:1715417/1‑65 (MQ=255) cAGTCGCAATTAGCGGTTTTTACCCGTATGCCGAAGTGAGCGGGATCTCAAGATTGTGGGATAgc < 1:935820/65‑1 (MQ=255) cGCAATTAGCGGTTTTTACCCGTATGCCGAAGTGAGCGGGATCTCAAGATTGTGGGATAgcggcg > 1:448294/1‑65 (MQ=255) cAATTAGCGGGTTTTACCCGTATGCCGAAGTGAGCGGGATCTCAAGATTGTGGGATAGCggcggc < 1:1324732/65‑1 (MQ=255) aaTTAGCGGTTTTTACCCGTATGCCGAAGTGAGCGGGATCTCAAGATTGTGGGATAGCGGCGGCt < 1:2566850/65‑1 (MQ=255) aTTAGCGGTTTTTACCCGTATGCCGAAGTGAGCGGGATCTCAAGATTGTGGGATAGCGGCGGCtt < 1:1548970/65‑1 (MQ=255) ttAGCGGTTTTTACTCGTATGCCGAAGTGTGCTTGATATCAAGATTGTGGGATAGCggcggcgct > 1:1808067/1‑62 (MQ=255) ttAGCGGTTTTTACCCGTATGCCGAAGTGTGCGTGATCTCAAGATTGTGGGATAGCGGCGGCttt > 1:2016088/1‑65 (MQ=255) ttAGCGGTTTTTACCCGTATGCCGAAGTGAGCGGGATCTCAAGATTGTGGGATAGCGGCGGGttt > 1:2485271/1‑65 (MQ=255) ttAGCGGTTTTTACCCGTATGCCGAAGTGAGCGGGATCTCAAGATTGTGGGATAGCGGCGGCttt > 1:645856/1‑65 (MQ=255) cGGTTTTTACCCGTATGCCGAAGTGAGCGGGATCTCAAGATTGTGGGATAGCGGCGGCTTTATGc < 1:3370644/65‑1 (MQ=255) cGGTTTTTACCCGTATGCCGAAGTGAGCGGGATCTCAAGATTGTGGGATAGCGGCGGCTCTATGc > 1:3555001/1‑65 (MQ=255) ggTTTTTGCCCGTATGCCGAAGTGAGCGGGATCTCAAGATTGTGGGATAGCGGCGGCTTTATGcc < 1:946150/65‑1 (MQ=255) aCCCGTATGTCGAAGTGAGCGGGATCTCAAGATTGTGGGATAGCGGCGGCTTTATGCCCAACGGt < 1:3210782/65‑1 (MQ=255) aCCCGTATGCCGAAGTGAGCGGGATCTCAAGATTGTGGGATAGCGGCGGCTTTATGCCCAGCGGt < 1:2421050/65‑1 (MQ=255) aCCCGTATGCCGAAGTGAGCGGGATCTCAAGATTGTGGGATAGCGGCGGCTTTATGCCCAACGGt < 1:3540463/65‑1 (MQ=255) aCCCGTATGCCGAAGTGAGCGGGATCTCAAGATTGTGGGATAGCGGCGGCTTTATGCCCAACGGt < 1:3396836/65‑1 (MQ=255) | GTGCAAAGTCATCGCTATCCTGGCCTTTATTTTCCTTGGTGCAGTCGCAATTAGCGGTTTTTACCCGTATGCCGAAGTGAGCGGGATCTCAAGATTGTGGGATAGCGGCGGCTTTATGCCCAACGGT > NC_000913/2794723‑2794849 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |