Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,390,754	G→T	A252A (GCC→GCA)	rsgA ←	ribosome small subunit‑dependent GTPase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,390,754	0	G	T	100.0%	91.5 / NA	26	A252A (GCC→GCA)	rsgA	ribosome small subunit‑dependent GTPase A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (16/10);  total (16/10)

ACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTT  >  NC_000913/4390693‑4390815                                                              |                                                              aCTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAgctgcg                                                            <  1:156948/65‑1 (MQ=255)   tCACGCACTCCTGGGGAGTGAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGt                                                          <  1:1594062/65‑1 (MQ=255)      cgcgCTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCgt                                                       <  1:2801087/65‑1 (MQ=255)           tCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGgtgt                                                  <  1:1304434/65‑1 (MQ=255)           tCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGgtgt                                                  <  1:3239407/65‑1 (MQ=255)            ccTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGtgtg                                                 <  1:3765691/65‑1 (MQ=255)            ccTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGtgtg                                                 <  1:1841373/65‑1 (MQ=255)            ccTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGtgtg                                                 >  1:3360617/1‑65 (MQ=255)               ggggAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGcgg                                              >  1:3560743/1‑63 (MQ=255)                 ggAGTCNATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGAc                                            >  1:2206448/1‑65 (MQ=255)                   agccAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                          >  1:2936325/4‑65 (MQ=255)                   aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                          >  1:2268246/1‑65 (MQ=255)                   aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                          >  1:3402276/1‑65 (MQ=255)                   aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                          >  1:1946599/1‑65 (MQ=255)                   aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                          >  1:714551/1‑65 (MQ=255)                   aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGGGCTGACCg                                          >  1:113213/1‑65 (MQ=255)                   aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGGCGTGGTGTGCTGACCg                                          >  1:3265430/1‑65 (MQ=255)                     tCAATCCCATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAg                                        <  1:502784/65‑1 (MQ=255)                           acatcacCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGa                                  >  1:1151298/1‑65 (MQ=255)                           acatcacCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGa                                  >  1:1985861/1‑65 (MQ=255)                                   gccgTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGGGTTGTCag                          >  1:1833027/1‑65 (MQ=255)                                   gccgTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCag                          >  1:2538303/1‑65 (MQ=255)                                   gccgTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCag                          >  1:3485283/1‑65 (MQ=255)                                    ccgTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGGCCGAGCCCCGAGTTGTCaga                         <  1:611975/65‑1 (MQ=255)                                      gTGCGGAAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAgaga                       <  1:369/65‑1 (MQ=255)                                                    gcAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAgg         <  1:3141719/64‑1 (MQ=255)                                                    aCAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAgg         >  1:2655928/1‑65 (MQ=255)                                                      aGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTCGTCAGAGATATCGTTGGTCAGGAt       >  1:3772439/1‑65 (MQ=255)                                                       gCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAtc      >  1:555102/1‑65 (MQ=255)                                                       gCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAtc      >  1:1605968/1‑65 (MQ=255)                                                         cGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGGTATCGTTGGTCAGGAtctc    <  1:979816/65‑1 (MQ=255)                                                           aGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCtt  <  1:3697411/65‑1 (MQ=255)                                                              |                                                              ACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTT  >  NC_000913/4390693‑4390815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑