Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	49,884	C→T	P21L (CCG→CTG)	folA →	dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	49,884	0	C	T	100.0%	45.8 / NA	14	P21L (CCG→CTG)	folA	dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/7);  total (7/7)

AATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAA  >  NC_000913/49822‑49931                                                               |                                                aaTGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGt                                               <  1:3159080/65‑1 (MQ=255) aaTGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGt                                               <  1:2833673/65‑1 (MQ=255) aaTGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGt                                               <  1:2021909/65‑1 (MQ=255)    gATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGa                                            <  1:2633545/65‑1 (MQ=255)     aTCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGaa                                           >  1:2998237/1‑65 (MQ=255)       cAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAAcc                                         <  1:972446/65‑1 (MQ=255)             gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAAcctgcctc                                   >  1:2183950/1‑64 (MQ=255)                     cgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGAtcgc                           >  1:555634/1‑63 (MQ=255)                      gTTAGCGGTCTATCGTTTTATCGGCATGGAAACCGCCATGTTGTGGACCCTCCCTGCCGATcgcg                          <  1:3209717/65‑1 (MQ=255)                            ggTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGACCCTGCCTGCCGATCGCGCCTGGt                    <  1:1425485/65‑1 (MQ=255)                               aGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCGCGCCTGGTTTa                 >  1:3500661/1‑65 (MQ=255)                                 aTCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCGCGCCTGGTTTaaa               >  1:250723/1‑65 (MQ=255)                                 aTCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCGCGCCTGGTTTaaa               >  1:1671916/1‑65 (MQ=255)                                 aTCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCGCGCCTGGTTTaaa               >  1:3154710/1‑65 (MQ=255)                                 aTCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCGCGCCTGGGTTaaa               >  1:1322577/1‑65 (MQ=255)                                 aTCGCGTTATCGGCATGGAAAACGCCATGCTGTAGAACCTGCCTGCCGATCGCGCCTGGTTTaaa               >  1:2600114/1‑65 (MQ=255)                                    gcgTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGc            >  1:2656662/1‑65 (MQ=255)                                        tATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAaca        <  1:306497/65‑1 (MQ=255)                                         aTCGTCATGGAAAACGCGGTGCTGTGGACCCTGCCTGCCGATCGCGCCTGGTTTAAACGGAacac       <  1:696106/65‑1 (MQ=255)                                         aTCGGCATGGAAACCGCCATGCTGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAacac       <  1:1313131/65‑1 (MQ=255)                                              cATGGAAAACGCCATGCTGGGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTtaa  <  1:1767867/65‑1 (MQ=255)                                              cATGAAAACCGCCGTGCTGTGGATCCAGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTtaa  <  1:794985/65‑1 (MQ=255)                                                               |                                                AATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAA  >  NC_000913/49822‑49931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑