Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	481,400	C→T	A1002T (GCA→ACA)	acrB ←	multidrug efflux pump RND permease AcrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	481,400	0	C	T	100.0%	82.0 / NA	24	A1002T (GCA→ACA)	acrB	multidrug efflux pump RND permease AcrB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/8);  total (16/8)

AACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAA  >  NC_000913/481338‑481460                                                               |                                                             aaCGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTgtgt                                                            <  1:1526434/65‑1 (MQ=255)           aTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGCTCGGCGCGcc                                                  >  1:515251/1‑65 (MQ=25)              gCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGa                                               >  1:3521828/1‑65 (MQ=255)               ccAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGaa                                              >  1:1810165/1‑65 (MQ=255)                cAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAAc                                             <  1:2144374/65‑1 (MQ=255)                    aCCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGc                                         >  1:1890394/1‑65 (MQ=255)                     ccGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCa                                        >  1:623781/1‑65 (MQ=255)                     ccGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCa                                        <  1:580903/65‑1 (MQ=255)                     ccGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCa                                        <  1:1669910/65‑1 (MQ=255)                       gTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCAcc                                      <  1:1427135/65‑1 (MQ=255)                          gCGGTCACCACCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGt                                   <  1:2585480/65‑1 (MQ=37)                             gTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACt                                <  1:535368/65‑1 (MQ=255)                              tCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTg                               >  1:3266016/1‑65 (MQ=255)                                          ccATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGc                   <  1:733260/65‑1 (MQ=255)                                          ccATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGc                   >  1:1750708/1‑65 (MQ=255)                                          ccATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGc                   >  1:849817/1‑65 (MQ=255)                                          ccATTACACCGGTACCTACTGTGTGCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGc                   <  1:2754518/65‑1 (MQ=255)                                                 acCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGc            >  1:2952103/1‑65 (MQ=255)                                                 acCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGc            <  1:2194468/65‑1 (MQ=255)                                                  ccGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCGCCAGTACTGATAACCAGCGGCATAACGcc           >  1:918331/1‑65 (MQ=25)                                                  ccGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGcc           >  1:3172239/1‑65 (MQ=255)                                                  ccGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGcc           >  1:3023718/1‑65 (MQ=255)                                                       aCCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGa      >  1:170989/1‑65 (MQ=255)                                                       aCCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGCACTGATAACCAGCGGCATAACGCCGAGGa      >  1:2856752/1‑65 (MQ=25)                                                         cTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACGAGCGGCATAACGCCGAGGATa    >  1:2037723/1‑65 (MQ=255)                                                           aCTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATaaa  >  1:342765/1‑65 (MQ=255)                                                           aCTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATaaa  >  1:308873/1‑65 (MQ=255)                                                               |                                                             AACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAA  >  NC_000913/481338‑481460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑