| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 485,614 | G→T | N2K (AAC→AAA) | acrA ← | multidrug efflux pump membrane fusion lipoprotein AcrA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 485,614 | 0 | G | T | 98.4% | 214.2 / ‑5.4 | 63 | N2K (AAC→AAA) | acrA | multidrug efflux pump membrane fusion lipoprotein AcrA |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base T (50/12); total (51/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
TTAGGGCTAAGCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTGTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAGTTAATAAAC > NC_000913/485553‑485674 | ttAGGGCTAAGCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTTTTc > 1:1868299/1‑65 (MQ=255) ggCTAAGCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTACACCCTCTGTTTTTTTTCatat > 1:2905100/1‑65 (MQ=255) ggCTAAGCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTTTTCatat < 1:1593341/65‑1 (MQ=255) gCTAAGCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTTTTCATATg > 1:1357535/1‑65 (MQ=255) cTAAGCTGCCTGCGAGCATCAGAACGACCGCCAGAGGCGTAAACACTCTGTTTTTTTTCATATGt > 1:41521/1‑65 (MQ=255) cTAAGCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTTTTCATATGt > 1:3143030/1‑65 (MQ=255) cgCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTTTTCATATGTaaa < 1:2651895/64‑1 (MQ=255) aGCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTTTTCATATGTaaa < 1:3002488/65‑1 (MQ=255) gCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTTTTCATATGTaacc > 1:489238/1‑63 (MQ=255) gCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTTTTCATATGTAAAc > 1:245204/1‑65 (MQ=255) gCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTTTTCATATGTAAAc > 1:639921/1‑65 (MQ=255) gCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTTTTCATATGTAAAc > 1:255374/1‑65 (MQ=255) gCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTTTTCATATGTAAAc > 1:1214788/1‑65 (MQ=255) gCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTTTTCATATGTAAAc > 1:3584816/1‑65 (MQ=255) gCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTTTTCATATGTAAAc > 1:1090572/1‑65 (MQ=255) gCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGGTTTTTTTCATATGTAAAc > 1:2054579/1‑65 (MQ=255) cTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTTTTCATATGTAAAcc < 1:2600777/65‑1 (MQ=255) tGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTTTTCATATGTAAACCt > 1:998465/1‑65 (MQ=255) agCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTc > 1:1050088/1‑65 (MQ=255) agCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTc > 1:2319541/1‑65 (MQ=255) agCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTGTTCATATGTAAACCTCGAGTGTc > 1:951874/1‑65 (MQ=255) aTCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTTTTCATATGTAAGCCTCGAGTGTCCgg < 1:432417/65‑2 (MQ=255) tCAGAACGACCGCCAGAGGAGTAAACCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGAt < 1:2659840/65‑1 (MQ=255) aaCGACCGCCAGAGGCGTAAACCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCa > 1:691992/1‑65 (MQ=255) aCGACCGCCAGAGGCGTAAACCCTCTGTTTTTTTTGATATGTAAACCTCGAGTGTCCGATTTCaa > 1:2874188/1‑65 (MQ=255) aCGACCGCCAGAGGCGTAAACCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCaa > 1:2012348/1‑65 (MQ=255) aCGACCGCCAGAGGCGTAAACCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCaa > 1:3299766/1‑65 (MQ=255) aCGACCGCCAGAGGCGTAAACCCTCTGTTTTTTTTCATATGNAAACCTCGAGTGTCCGATTTCaa > 1:2932825/1‑65 (MQ=255) gagGCGTAAACCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATtggtcaat > 1:3300944/1‑65 (MQ=255) gagGCGTAAACCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATtggtcaat > 1:3606797/1‑65 (MQ=255) gagGCGTAAACCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATtggtcaat > 1:1754639/1‑65 (MQ=255) gagGCGTAAACCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATtagtcaat > 1:1960941/1‑65 (MQ=255) ggCGTAAACGCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATtggncaatgg < 1:2213076/65‑1 (MQ=255) ggCGTAAACCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATtggtcaatgg > 1:1903559/1‑65 (MQ=255) ggCGTAAACCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATtggtcaatgg > 1:1231499/1‑65 (MQ=255) cGTAAACCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATtggtcaatggtc > 1:3343306/1‑65 (MQ=255) cGTAAACCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATtggtcaatggtc > 1:677776/1‑65 (MQ=255) cGTAAACCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATtggtcaatggtc > 1:2884491/1‑65 (MQ=255) cGTAAACCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATtggtcaatggtc > 1:1046956/1‑65 (MQ=255) aaaCCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCaaa < 1:1017810/65‑1 (MQ=255) aaaCCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCaaa < 1:1408263/65‑1 (MQ=255) aCCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAg > 1:395267/1‑65 (MQ=255) aCCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAg > 1:1163610/1‑65 (MQ=255) aCCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAg > 1:841219/1‑65 (MQ=255) aCCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAg > 1:733697/1‑65 (MQ=255) aCCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAg > 1:691765/1‑65 (MQ=255) aCCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAg > 1:1375183/1‑65 (MQ=255) aCCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAg > 1:1401549/1‑65 (MQ=255) aCCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAg > 1:494191/1‑65 (MQ=255) aCCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAg > 1:490163/1‑65 (MQ=255) aCCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAg > 1:1881092/1‑65 (MQ=255) aCCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAg > 1:392192/1‑65 (MQ=255) aCCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAg > 1:3622264/1‑65 (MQ=255) aCCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAg > 1:30111/1‑65 (MQ=255) aCCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAg > 1:3436508/1‑65 (MQ=255) aCCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAg > 1:3170424/1‑65 (MQ=255) aCCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAg > 1:2602244/1‑65 (MQ=255) aCCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAg > 1:2460573/1‑65 (MQ=255) aCCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGCCAAAAg > 1:1979827/1‑65 (MQ=255) aCCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGACCGATTTCAAATTGGTCAATGGTCAAAAg > 1:2868084/1‑65 (MQ=255) aCCCTCTGTTTGTTTTCANATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAg > 1:2772820/1‑65 (MQ=255) cccTCTGTTTTTTTTCATCTGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAGt > 1:2787585/1‑65 (MQ=255) cccTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAGt > 1:1823229/1‑65 (MQ=255) cccTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAGt > 1:2436981/1‑65 (MQ=255) ccTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAGtt < 1:1236260/65‑1 (MQ=255) ttctGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAGTta < 1:40649/64‑1 (MQ=255) ctctGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAGTta < 1:319033/65‑1 (MQ=255) ctctGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAGTta < 1:2971192/65‑1 (MQ=255) ctctGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAGTta < 1:808149/65‑1 (MQ=255) ttttttTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAGTTAATAAAc < 1:534387/65‑1 (MQ=255) ttttttTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAGTTAATAAAc < 1:779772/65‑1 (MQ=255) ttttttTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAGTTAATAAAc < 1:2651129/65‑1 (MQ=255) ttttttTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAGTTAATAAAc < 1:3730906/65‑1 (MQ=255) ttttttTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAGTTAATAAAc > 1:2031254/1‑65 (MQ=255) cttttttCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAGTTAATAAAc < 1:2645119/64‑1 (MQ=255) | TTAGGGCTAAGCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTGTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAGTTAATAAAC > NC_000913/485553‑485674 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |