| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 421,152 | 0 | A | . | 33.3% | 34.2 / 15.7 | 15 | coding (167/1374 nt) | proY | putative transporter ProY |
| Reads supporting (aligned to +/- strand): ref base A (4/6); new base . (0/5); total (4/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.31e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCG > NC_000913/421089‑421215 | aCGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGtat < 1:3216015/65‑1 (MQ=255) gCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTatat < 1:3540986/65‑1 (MQ=255) cATCAAAATGGCCGGTCCGAGCGTGTTGTTTGCCTATATTATCGGTGGTATCGCGGCGTATATCa < 1:2685827/65‑1 (MQ=255) aaaTGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTAtg < 1:2232504/65‑1 (MQ=255) gCCGGTCCGAGCGTTTTGTTGGCCTAAATTATCGGTGG‑‑TCGC‑‑CG‑‑TATCATTATGCGTGCGCTggg < 1:2642945/65‑1 (MQ=255) ccGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTggg < 1:2418316/65‑1 (MQ=255) gtgtTGTTGGCTTATATTATCGGTGG‑‑TCGC‑‑CG‑‑TATCATTATGCGTGCGCTGGGGGAAATGTCGGt < 1:1560951/65‑1 (MQ=255) gtgtTGTGGGCTTATATTATCGGTGG‑‑TCGC‑‑CG‑‑TATCATTATGCGTGCGCTGGGGGAAATGTCGGt < 1:1308602/65‑1 (MQ=255) tgttgtTGGCCTATATTATCGGTGG‑‑TCGC‑‑CG‑‑TATCATTATGCGTGCGCTGGGGGAAATGTCGGTa < 1:3632589/65‑1 (MQ=255) gttgttGGCCTATATTATCGGTGG‑‑TCGC‑‑CG‑‑TATCATTATGCGTGCGCTGGGGGAAATGTCGGTAc < 1:1622585/65‑1 (MQ=255) tgttgGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCg > 1:1884534/1‑65 (MQ=255) ttgGCCTATATCACCGGCGGTATCGCGGCGTTTGTCATTATGCGTGCGCTGGGGGAAATGTCGGt < 1:3492127/65‑1 (MQ=255) tgGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTa < 1:3291716/65‑1 (MQ=255) ggCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTAc < 1:1355533/65‑1 (MQ=255) atatTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAAc < 1:2454550/65‑1 (MQ=255) cGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGgccg > 1:676609/1‑65 (MQ=255) cGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGgccg > 1:762341/1‑65 (MQ=255) cgcgGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCtt > 1:2425279/1‑65 (MQ=255) gcggcgTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCttt < 1:408801/65‑1 (MQ=255) tatataGTTATGCGTGCGCGGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTcgcg < 1:2317021/65‑1 (MQ=255) | ACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCG > NC_000913/421089‑421215 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |