| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 424,518 | 0 | A | C | 38.9% | 18.9 / 12.7 | 18 | V134G (GTG→GGG) | acpH | acyl carrier protein phosphodiesterase |
| Reads supporting (aligned to +/- strand): ref base A (11/0); new base C (0/7); total (11/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.14e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGTGGGCGGCGGCTTGCCATGCCGTTTAACACGTTCTGGATGAAAT‑CCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAA > NC_000913/424455‑424582 | cGTGGGCGGCGGCTTGCCATGCCGTTTAACACGTTCTGGATGAAAT‑CCATATCGCGATAGCGCAc > 1:1264372/1‑65 (MQ=255) gcggcggcTTGCCATGCCGTTTAACACGTTCTGGATGAAAT‑CCATATCGCGATAGCGCACCAGcc > 1:777082/1‑65 (MQ=255) gcggcggcTTGCCATGCCGTTTAACACGTTCTGGATGAAAT‑CCATATCGCGATAGCGCACCAGcc > 1:2829905/1‑65 (MQ=255) gCCATGCCGTTTAACACGTTCTGGATGAAAT‑CCATATCGCGATAGCGCCCCAGCCACTGCTCTGa < 1:41492/65‑1 (MQ=255) gCCATGCCGTTTAACACGTTCTGAATGAAAT‑CCATATCGCGATAGCCCCCCAGCCACTGCTCTGa < 1:384745/65‑1 (MQ=255) gCCAGGCCGTTTAACACTTTCTGGATGAAAT‑CCATACCGGGATAGCGCCCCAGCCACTGCTCTGa < 1:2215112/65‑1 (MQ=255) cccATGCCGTTTAACACTTTCTGGATGAAAT‑CCATATCGCGATAGCGCCCCAGCCACTGCTCTGa < 1:1337941/64‑1 (MQ=255) cagaTGCCTTTTAACACGTTCTGGATAAAAT‑CAATATCGCGATAGCCCCCCAGCCACTGCTCTGa < 1:1764448/62‑1 (MQ=255) ccATGCCGTTTAACACGTTCTGGATGAAAT‑CCATATCGCGATAGCGCACCAGCCACTGCTCTGAc > 1:1837915/1‑65 (MQ=255) ttAACACGTTCTGGATGAAAT‑CCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTaa > 1:163219/1‑65 (MQ=255) aaCACGTTCTGGATGAAAT‑CCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAAtt > 1:67676/1‑65 (MQ=255) caggTTCTGGA‑GAAATCCCATATCGCGAGAGCGCCCCAGCCACTGCTCTGACCACAAGTAATTGt < 1:1970720/62‑1 (MQ=255) cacTTTCTGGATAAAAT‑CCATATCGCGATAGCCCCCCAGCCACTGCTCTGACCACAAGTAATTGt < 1:1946001/65‑1 (MQ=255) cacGTTCTGGATGAAAT‑CCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGt > 1:233137/1‑65 (MQ=255) cacGTCCTGGATGAATT‑CCATACCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGt < 1:1103282/65‑1 (MQ=255) acggTTCTGGAAAAAAC‑CAATACCGCGATAGCCCCCCAGCCACTGCTCTGACCACAAGTAATTGt < 1:453055/62‑1 (MQ=255) acGTTCTGGATGAAAT‑CCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGtt > 1:260382/1‑65 (MQ=255) gATGAAAT‑CCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGa > 1:1718303/1‑65 (MQ=255) t‑CCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACg > 1:846496/1‑65 (MQ=255) aTAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAgc > 1:2691506/1‑64 (MQ=255) aGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTcc > 1:2134904/1‑65 (MQ=255) gcACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGc < 1:1776364/65‑1 (MQ=255) aCCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCaa > 1:3624410/1‑65 (MQ=255) | CGTGGGCGGCGGCTTGCCATGCCGTTTAACACGTTCTGGATGAAAT‑CCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAA > NC_000913/424455‑424582 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |